LOCUS       sequence-232292-        3878 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial mWasabi promoter trap.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Kang YS, Kirby JE
  TITLE     Promotion and Rescue of Intracellular Brucella neotomae Replication 
            during Coinfection with Legionella pneumophila.
  JOURNAL   Infect Immun. 2017 Apr 21;85(5). pii: e00991-16. doi: 
            10.1128/IAI.00991-16. Print 2017 May.
  PUBMED    28264909
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Infect 
            Immun."; date: "2017-04-21"; volume: "85(5). pii: e00991-16. doi"; 
            pages: " 10.1128/IAI.00991-16. Print 2017 May"
COMMENT     Sequence Label: pMOD3-mWasabi-Sh_ble
FEATURES             Location/Qualifiers
     source          1..3878
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    309..327
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     misc_feature    1804..1822
                     /label=Tn5 ME
                     /note="hyperactive mosaic end for Tn5 transposase 
                     recognition (Reznikoff et al., 2004)"
     primer_bind     complement(1888..1905)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2059..2647)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2139..2158)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2818..3609,3610..3678))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3441..3460
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3679..3783)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3851..3869
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgacagct gtctcttata cacatctcaa ccatcatcga tgaattttct cgggtgttct
      361 cgcatattgg ctcgaattct aaggaggaaa aaaaatggtg agtaaaggcg aagaaaccac
      421 catgggcgtg attaaaccag atatgaaaat caagctgaaa atggaaggta atgttaacgg
      481 ccatgcattt gtaattgaag gcgagggaga gggcaagccg tatgatggta cgaatacgat
      541 caatctggag gttaaggaag gcgcgccttt acccttttcc tatgatatcc tgaccaccgc
      601 cttttcgtat ggtaatcgcg cattcacgaa atatccggac gatatcccga actatttcaa
      661 acagagcttt ccggaaggtt atagctggga acgtaccatg accttcgagg ataagggcat
      721 cgtgaaagtg aaaagcgata tttccatgga agaagattct ttcatctacg agatccatct
      781 gaaaggcgaa aacttcccgc caaacggtcc agtgatgcag aaagagacga cgggctggga
      841 tgcgagcacg gagcgtatgt atgttcgcga cggcgtgctg aagggtgatg tcaagatgaa
      901 actcctgctg gaaggcggtg gccaccaccg tgtggatttc aagacgattt accgcgccaa
      961 gaaggcggtc aaactgccag actaccactt tgtggaccat cgtattgaaa tccttaatca
     1021 tgataaagac tataacaaag tgaccgtata cgagattgca gtggcacgca attccacgga
     1081 cggcatggac gaactttaca aataagtcga cggaggacag taatggccaa gttgaccagt
     1141 gccgttccgg tgctgaccgc acgtgacgtc gccggagcag tggagttctg gaccgaccgt
     1201 ctggggttct cccgtgactt cgtggaggac gacttcgccg gtgtggtccg tgacgacgtg
     1261 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
     1321 tgggtgcgtg gcctggacga gctgtacgcc gagtggagcg aagtcgtgtc cacgaacttc
     1381 cgtgacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgtgagttc
     1441 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgaaag
     1501 ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta gaggctattt
     1561 aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac gtgaaacatg
     1621 agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt tagttcgtta
     1681 aacatgagag cttagtacgt taaacatgag agcttagtac gtgaaacatg agagcttagt
     1741 acgtactatc aacaggttga actgccaacg actacgcact agccaacaag agcttcaggg
     1801 ttgagatgtg tataagagac agctgtctta atgaatcggc caacgcgcgg ggagaggcgg
     1861 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     1921 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     1981 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2041 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2101 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2161 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2221 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2281 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2341 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2401 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2461 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     2521 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2581 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2641 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2701 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2761 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2821 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     2881 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     2941 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3001 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3061 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3121 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3181 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3241 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3301 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3361 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3421 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3481 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3541 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3601 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3661 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3721 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3781 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt
     3841 aacctataaa aataggcgta tcacgaggcc ctttcgtc
//
