LOCUS       sequence-232306-        6638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses Jaws-mRuby2 fusion in GABAergic inhibitory neurons in 
            rodent cortex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6638)
  AUTHORS   Keaveney MK, Tseng HA, Ta TL, Gritton HJ, Man HY, Han X
  TITLE     A MicroRNA-Based Gene-Targeting Tool for Virally Labeling 
            Interneurons in the Rodent Cortex.
  JOURNAL   Cell Rep. 2018 Jul 10;24(2):294-303. doi: 
            10.1016/j.celrep.2018.06.049.
  PUBMED    29996091
REFERENCE   2  (bases 1 to 6638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2018.06"; journalName: "Cell Rep"; date: 
            "2018-07-10- 10"; volume: "24"; issue: "2"; pages: "294-303"
COMMENT     Sequence Label: AAV-hSyn-Jaws-mRuby2-8X2C
FEATURES             Location/Qualifiers
     source          1..6638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     promoter        224..671
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             1530..2237
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     misc_feature    2768..3356
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2821..2841)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3239..3250)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3359..3375)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3360..3376)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3405..3526
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3416..3435)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3470..3489
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3688..3817
                     /label=AAV2 ITR
     primer_bind     complement(3836..3853)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3836..3852)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3845..3867)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3994..4449
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4081..4100)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4291..4312
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4475..4579
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4580..4648,4649..5440)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4798..4817)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5611..6199
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6100..6119
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6487..6508
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6523..6528,6529..6546,6547..6553)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6561..6577
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6566..6588
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6585..6601
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6585..6601
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatct gaattctcta gactgcagag ggccctgcgt atgagtgcaa gtgggtttta
      241 ggaccaggat gaggcggggt gggggtgcct acctgacgac cgaccccgac ccactggaca
      301 agcacccaac ccccattccc caaattgcgc atcccctatc agagaggggg aggggaaaca
      361 ggatgcggcg aggcgcgtgc gcactgccag cttcagcacc gcggacagtg ccttcgcccc
      421 cgcctggcgg cgcgcgccac cgccgcctca gcactgaagg cgcgctgacg tcactcgccg
      481 gtcccccgca aactcccctt cccggccacc ttggtcgcgt ccgcgccgcc gccggcccag
      541 ccggaccgca ccacgcgagg cgcgagatag gggggcacgg gcgcgaccat ctgcgctgcg
      601 gcgccggcga ctcagcgctg cctcagtctg cggtgggcag cggaggagtc gtgtcgtgcc
      661 tgagagcgca gtcgagagga tccgccgcca ccatgaccgc cgtgagcacc acagccacta
      721 ccgtgctgca ggccacacag agcgacgtgc tgcaggagat ccagtccaac ttcctgctga
      781 atagctccat ctgggtgaac attgctctgg ccggagtggt catcctgctg tttgtggcca
      841 tggggaggga tctggaatcc cctagagcta agctgatctg ggtggccaca atgctggtgc
      901 cactggtgtc tatttctagt tacgctggac tggccagtgg gctgactgtg ggcttcctgc
      961 agatgccacc tggacacgct ctggccggac aggaggtgct gagcccatgg ggccggtatc
     1021 tgacatggac tttctccact cccatgatcc tgctggctct gggactgctg gccgacaccg
     1081 atattgccag cctgttcacc gccatcacaa tggacattgg catgtgcgtg acaggactgg
     1141 ccgctgccct gatcactagc tcccatctgc tgcgctgggt gttctacgga atttcttgtg
     1201 ctttctttgt ggccgtgctg tatgtgctgc tggtgcagtg gccagctgat gctgaggctg
     1261 ctgggaccag tgaaatcttt ggcactctga ggattctgac cgtggtgctg tggctggggt
     1321 accctatcct gttcgctctg ggctctgagg gagtggccct gctgagtgtg ggagtgacca
     1381 gctggggata ctccggactg gacatcctgg ctaaatacgt gttcgccttt ctgctgctga
     1441 gatgggtggc tgccaatgaa ggcacagtgt ctgggagtgg aatgggaatc gggtccggag
     1501 gagctgctcc agccgacgat cgaccggtag tgtctaaggg cgaagagctg atcaaggaaa
     1561 atatgcgtat gaaggtggtc atggaaggtt cggtcaacgg ccaccaattc aaatgcacag
     1621 gtgaaggaga aggcaatccg tacatgggaa ctcaaaccat gaggatcaaa gtcatcgagg
     1681 gaggacccct gccatttgcc tttgacattc ttgccacgtc gttcatgtat ggcagccgta
     1741 cttttatcaa gtacccgaaa ggcattcctg atttctttaa acagtccttt cctgagggtt
     1801 ttacttggga aagagttacg agatacgaag atggtggagt cgtcaccgtc atgcaggaca
     1861 ccagccttga ggatggctgt ctcgtttacc acgtccaagt cagaggggta aactttccct
     1921 ccaatggtcc cgtgatgcag aagaagacca agggttggga gcctaataca gagatgatgt
     1981 atccagcaga tggtggtctg aggggataca ctcatatggc actgaaagtt gatggtggtg
     2041 gccatctgtc ttgctctttc gtaacaactt acaggtcaaa aaagaccgtc gggaacatca
     2101 agatgcccgg tatccatgcc gttgatcacc gcctggaaag gttagaggaa agtgacaatg
     2161 aaatgttcgt agtacaacgc gaacacgcag ttgccaagtt cgccgggctt ggtggtggga
     2221 tggacgagct gtacaagctg tacaagttct gctacgagaa tgaagtgtaa actagtgcgg
     2281 ccttaaagag accggttcac tgtgacagta aaagagaccg gttcactgtg agaatgaaag
     2341 agaccggttc actgtgatcg gaaaagagac cggttcactg tgagcggcct taaagagacc
     2401 ggttcactgt gacagtaaaa gagaccggtt cactgtgaga atgaaagaga ccggttcact
     2461 gtgatcggaa aagagaccgg ttcactgtga gcggccttga aacccagcag acaatgtagc
     2521 tcagtagaaa cccagcagac aatgtagctg aatggaaacc cagcagacaa tgtagcttcg
     2581 gagaaaccca gcagacaatg tagctgcggc cttgaaaccc agcagacaat gtagctcagt
     2641 agaaacccag cagacaatgt agctgaatgg aaacccagca gacaatgtag cttcggagaa
     2701 acccagcaga caatgtagct gcggccgcga ctctagagtc gacctgcagg catgcaagct
     2761 tatcgataat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     2821 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     2881 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     2941 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     3001 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     3061 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     3121 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     3181 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     3241 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     3301 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg
     3361 ataccgtcga cctcgacccg ggcggccgct tcgagcagac atgataagat acattgatga
     3421 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     3481 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     3541 cattcatttt atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa
     3601 cctctacaaa tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt
     3661 agcatggcgg gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct
     3721 ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct
     3781 ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct taattaacct aattcactgg
     3841 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3901 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3961 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     4021 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     4081 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     4141 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     4201 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     4261 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     4321 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     4381 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     4441 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4501 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4561 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4621 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4681 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4741 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4801 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4861 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4921 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4981 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     5041 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5101 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5161 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5221 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5281 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5341 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5401 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5461 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5521 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5581 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5641 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5701 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5761 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5821 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5881 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5941 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     6001 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     6061 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     6121 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     6181 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     6241 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     6301 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     6361 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     6421 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     6481 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     6541 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     6601 ccatgattac gccagattta attaaggcct taattagg
//
