LOCUS       sequence-232316-        8980 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Base editor made from APOBEC3D.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8980)
  AUTHORS   Martin AS, Salamango DJ, Serebrenik AA, Shaban NM, Brown WL, Harris 
            RS
  TITLE     A panel of eGFP reporters for single base editing by APOBEC-Cas9 
            editosome complexes.
  JOURNAL   Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1038/s41598-018-36739-9.
  PUBMED    30679582
REFERENCE   2  (bases 1 to 8980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2019-01-24"; volume: "9(1):497. doi"; pages: " 
            10.1038/s41598-018-36739-9"
COMMENT     Sequence Label: A3D-Cas9n-UGI
FEATURES             Location/Qualifiers
     source          1..8980
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        132..335
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     286..306
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             join(1592..4990,4991..4993,4994..5590,5591,5592..5594,
                     5595..5596,5597..5599,5600..5692)
                     /codon_start=1
                     /product="Streptococcus pyogenes Cas9 endonuclease with the
                     D1135V/R1335Q/T1337R mutations"
                     /label=Cas9 VQR
                     /note="has an altered recognition specificity for the 
                     protospacer adjacent motif (PAM) (Kleinstiver et al., 
                     2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             join(1592..1615,1616..1618,1619..5692)
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the 
                     Streptococcus pyogenes Type II CRISPR/Cas system"
                     /label=Cas9(D10A)
                     /note="generates RNA-guided single strand nicks in DNA due 
                     to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5705..5953
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus 
                     subtilis bacteriophage (Mol et al., 1995)"
                     /label=UGI
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
                     YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
     CDS             5966..5986
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5995..6012
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6035..6052)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6041..6265
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6336..6352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6336..6352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6349..6371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6360..6376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6384..6390,6391..6408,6409..6414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6429..6450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6567..6584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6738..7326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6818..6837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7497..8288,8289..8357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8120..8139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8358..8462)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8541..8560)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(8732..8980,1..131)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
       61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
      121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
      181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
      241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
      301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
      361 agagatccgc ggccgcgccg ccaccatgaa tccacagatc agaaatccga tggagcggat
      421 gtatcgagac acattctacg acaactttga aaacgaaccc atcctctatg gtcggagcta
      481 cacttggctg tgctatgaag tgaaaataaa gaggggccgc tcaaatctcc tttgggacac
      541 aggggtcttt cgaggcccgg tactacccaa acgtcagtcg aatcacaggc aggaggtgta
      601 tttccggttt gagaaccacg cagaaatgtg cttcttatct tggttctgtg gcaaccgact
      661 gcctgctaac aggcgcttcc agatcacctg gtttgtatca tggaacccct gcctgccctg
      721 tgtggtgaag gtgaccaaat tcttggctga gcaccccaat gtcaccctga ccatctctgc
      781 cgcccgcctc tactactacc gggatagaga ttggcggtgg gtgctcctca ggctgcataa
      841 ggcaggggcc cgtgtgaaga tcatggacta tgaagacttt gcatactgct gggaaaactt
      901 tgtgtgcaat gaaggtcagc cattcatgcc ttggtacaaa ttcgatgaca attatgcatc
      961 cctgcaccgc acgctaaagg agattctcag aaacccgatg gaggcaatgt acccacacat
     1021 attctacttc cactttaaaa acctactgaa agcctgtggt cggaacgaaa gctggctgtg
     1081 cttcaccatg gaagttacaa agcaccactc agctgtcttc cggaagaggg gcgtcttccg
     1141 aaaccaggtg gatcctgaga cccattgtca tgcagaaagg tgcttcctct cttggttctg
     1201 tgacgacata ctgtctccta acacaaacta cgaggtcacc tggtacacat cttggagccc
     1261 ttgcccagag tgtgcagggg aggtggccga gttcctggcc aggcacagca acgtgaatct
     1321 caccatcttc accgcccgcc tctgctattt ctgggataca gattaccagg aggggctctg
     1381 cagcctgagt caggaagggg cctccgtgaa gatcatgggc tacaaagatt ttgtatcttg
     1441 ttggaaaaac tttgtgtaca gtgatgatga gccattcaag ccttggaagg gactacaaac
     1501 caactttcga cttctgaaaa gaaggctacg ggagattctc cagagcggca gcgagactcc
     1561 cgggacctca gagtccgcca cacccgaaag tgataaaaag tattctattg gtttagccat
     1621 cggcactaat tccgttggat gggctgtcat aaccgatgaa tacaaagtac cttcaaagaa
     1681 atttaaggtg ttggggaaca cagaccgtca ttcgattaaa aagaatctta tcggtgccct
     1741 cctattcgat agtggcgaaa cggcagaggc gactcgcctg aaacgaaccg ctcggagaag
     1801 gtatacacgt cgcaagaacc gaatatgtta cttacaagaa atttttagca atgagatggc
     1861 caaagttgac gattctttct ttcaccgttt ggaagagtcc ttccttgtcg aagaggacaa
     1921 gaaacatgaa cggcacccca tctttggaaa catagtagat gaggtggcat atcatgaaaa
     1981 gtacccaacg atttatcacc tcagaaaaaa gctagttgac tcaactgata aagcggacct
     2041 gaggttaatc tacttggctc ttgcccatat gataaagttc cgtgggcact ttctcattga
     2101 gggtgatcta aatccggaca actcggatgt cgacaaactg ttcatccagt tagtacaaac
     2161 ctataatcag ttgtttgaag agaaccctat aaatgcaagt ggcgtggatg cgaaggctat
     2221 tcttagcgcc cgcctctcta aatcccgacg gctagaaaac ctgatcgcac aattacccgg
     2281 agagaagaaa aatgggttgt tcggtaacct tatagcgctc tcactaggcc tgacaccaaa
     2341 ttttaagtcg aacttcgact tagctgaaga tgccaaattg cagcttagta aggacacgta
     2401 cgatgacgat ctcgacaatc tactggcaca aattggagat cagtatgcgg acttattttt
     2461 ggctgccaaa aaccttagcg atgcaatcct cctatctgac atactgagag ttaatactga
     2521 gattaccaag gcgccgttat ccgcttcaat gatcaaaagg tacgatgaac atcaccaaga
     2581 cttgacactt ctcaaggccc tagtccgtca gcaactgcct gagaaatata aggaaatatt
     2641 ctttgatcag tcgaaaaacg ggtacgcagg ttatattgac ggcggagcga gtcaagagga
     2701 attctacaag tttatcaaac ccatattaga gaagatggat gggacggaag agttgcttgt
     2761 aaaactcaat cgcgaagatc tactgcgaaa gcagcggact ttcgacaacg gtagcattcc
     2821 acatcaaatc cacttaggcg aattgcatgc tatacttaga aggcaggagg atttttatcc
     2881 gttcctcaaa gacaatcgtg aaaagattga gaaaatccta acctttcgca taccttacta
     2941 tgtgggaccc ctggcccgag ggaactctcg gttcgcatgg atgacaagaa agtccgaaga
     3001 aacgattact ccatggaatt ttgaggaagt tgtcgataaa ggtgcgtcag ctcaatcgtt
     3061 catcgagagg atgaccaact ttgacaagaa tttaccgaac gaaaaagtat tgcctaagca
     3121 cagtttactt tacgagtatt tcacagtgta caatgaactc acgaaagtta agtatgtcac
     3181 tgagggcatg cgtaaacccg cctttctaag cggagaacag aagaaagcaa tagtagatct
     3241 gttattcaag accaaccgca aagtgacagt taagcaattg aaagaggact actttaagaa
     3301 aattgaatgc ttcgattctg tcgagatctc cggggtagaa gatcgattta atgcgtcact
     3361 tggtacgtat catgacctcc taaagataat taaagataag gacttcctgg ataacgaaga
     3421 gaatgaagat atcttagaag atatagtgtt gactcttacc ctctttgaag atcgggaaat
     3481 gattgaggaa agactaaaaa catacgctca cctgttcgac gataaggtta tgaaacagtt
     3541 aaagaggcgt cgctatacgg gctggggacg attgtcgcgg aaacttatca acgggataag
     3601 agacaagcaa agtggtaaaa ctattctcga ttttctaaag agcgacggct tcgccaatag
     3661 gaactttatg cagctgatcc atgatgactc tttaaccttc aaagaggata tacaaaaggc
     3721 acaggtttcc ggacaagggg actcattgca cgaacatatt gcgaatcttg ctggttcgcc
     3781 agccatcaaa aagggcatac tccagacagt caaagtagtg gatgagctag ttaaggtcat
     3841 gggacgtcac aaaccggaaa acattgtaat cgagatggca cgcgaaaatc aaacgactca
     3901 gaaggggcaa aaaaacagtc gagagcggat gaagagaata gaagagggta ttaaagaact
     3961 gggcagccag atcttaaagg agcatcctgt ggaaaatacc caattgcaga acgagaaact
     4021 ttacctctat tacctacaaa atggaaggga catgtatgtt gatcaggaac tggacataaa
     4081 ccgtttatct gattacgacg tcgatcacat tgtaccccaa tcctttttga aggacgattc
     4141 aatcgacaat aaagtgctta cacgctcgga taagaaccga gggaaaagtg acaatgttcc
     4201 aagcgaggaa gtcgtaaaga aaatgaagaa ctattggcgg cagctcctaa atgcgaaact
     4261 gataacgcaa agaaagttcg ataacttaac taaagctgag aggggtggct tgtctgaact
     4321 tgacaaggcc ggatttatta aacgtcagct cgtggaaacc cgccaaatca caaagcatgt
     4381 tgcacagata ctagattccc gaatgaatac gaaatacgac gagaacgata agctgattcg
     4441 ggaagtcaaa gtaatcactt taaagtcaaa attggtgtcg gacttcagaa aggattttca
     4501 attctataaa gttagggaga taaataacta ccaccatgcg cacgacgctt atcttaatgc
     4561 cgtcgtaggg accgcactca ttaagaaata cccgaagcta gaaagtgagt ttgtgtatgg
     4621 tgattacaaa gtttatgacg tccgtaagat gatcgcgaaa agcgaacagg agataggcaa
     4681 ggctacagcc aaatacttct tttattctaa cattatgaat ttctttaaga cggaaatcac
     4741 tctggcaaac ggagagatac gcaaacgacc tttaattgaa accaatgggg agacaggtga
     4801 aatcgtatgg gataagggcc gggacttcgc gacggtgaga aaagttttgt ccatgcccca
     4861 agtcaacata gtaaagaaaa ctgaggtgca gaccggaggg ttttcaaagg aatcgattct
     4921 tccaaaaagg aatagtgata agctcatcgc tcgtaaaaag gactgggacc cgaaaaagta
     4981 cggtggcttc gatagcccta cagttgccta ttctgtccta gtagtggcaa aagttgagaa
     5041 gggaaaatcc aagaaactga agtcagtcaa agaattattg gggataacga ttatggagcg
     5101 ctcgtctttt gaaaagaacc ccatcgactt ccttgaggcg aaaggttaca aggaagtaaa
     5161 aaaggatctc ataattaaac taccaaagta tagtctgttt gagttagaaa atggccgaaa
     5221 acggatgttg gctagcgccg gagagcttca aaaggggaac gaactcgcac taccgtctaa
     5281 atacgtgaat ttcctgtatt tagcgtccca ttacgagaag ttgaaaggtt cacctgaaga
     5341 taacgaacag aagcaacttt ttgttgagca gcacaaacat tatctcgacg aaatcataga
     5401 gcaaatttcg gaattcagta agagagtcat cctagctgat gccaatctgg acaaagtatt
     5461 aagcgcatac aacaagcaca gggataaacc catacgtgag caggcggaaa atattatcca
     5521 tttgtttact cttaccaacc tcggcgctcc agccgcattc aagtattttg acacaacgat
     5581 agatcgcaaa cgatacactt ctaccaagga ggtgctagac gcgacactga ttcaccaatc
     5641 catcacggga ttatatgaaa ctcggataga tttgtcacag cttgggggtg actctggtgg
     5701 ttctactaat ctgtcagata ttattgaaaa ggagaccggt aagcaactgg ttatccagga
     5761 atccatcctc atgctcccag aggaggtgga agaagtcatt gggaacaagc cggaaagcga
     5821 tatactcgtg cacaccgcct acgacgagag caccgacgag aatgtcatgc ttctgactag
     5881 cgacgcccct gaatacaagc cttgggctct ggtcatacag gatagcaacg gtgagaacaa
     5941 gattaagatg ctctctggtg gttctcccaa gaagaagagg aaagtctaac cggtcatcat
     6001 caccatcacc attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag
     6061 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
     6121 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
     6181 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
     6241 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgata
     6301 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     6361 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctag
     6421 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     6481 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     6541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     6601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     6661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     6721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     6781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     6841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     6901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     6961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     7021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     7081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     7141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     7201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     7261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     7321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     7381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     7441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     7501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     7561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     7621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     7681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     7741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     7801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     7861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     7921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     7981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     8041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
     8101 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
     8161 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
     8221 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
     8281 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
     8341 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
     8401 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
     8461 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg atctcccgat
     8521 cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag ccagtatctg
     8581 ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta agctacaaca
     8641 aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg
     8701 cttcgcgatg tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag
     8761 taatcaatta cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt
     8821 acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg
     8881 acgtatgttc ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat
     8941 ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
//
