LOCUS       sequence-232688-        5783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculovirus expression for structure determination.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5783)
  TITLE     SGC Toronto Q3-Q4 2017, Q1-Q2 2018
REFERENCE   2  (bases 1 to 5783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: 6CVZ (RFWD3)
FEATURES             Location/Qualifiers
     source          1..5783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..92
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     17..34
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     CDS             151..168
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             181..201
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     polyA_signal    1389..1523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1426..1445)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1480..1499
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  1552..1717
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      1901..2355
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1988..2007)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2197..2218
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2381..2485
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2486..2554,2555..3346)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2704..2723)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3517..4105
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4006..4025
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4259..4276
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  4410..4634
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4701..5234)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(5404..5409,5410..5426,5427..5432))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 atcatggaga taattaaaat gataaccatc tcgcaaataa ataagtattt tactgttttc
       61 gtaacagttt tgtaataaaa aaacctataa atattccgga ttattcatac cgtcccacca
      121 tcgggcgcgg atctcggtcc gaaaaccatg catcatcatc atcatcacag cagcggcaga
      181 gaaaacttgt atttccaggg ctcaccctcc agccagggcc agcacaagca caagtaccac
      241 ttccaaaaga ccttcacagt atctcaggca ggaaactgcc ggatcatggc atactgtgat
      301 gctctgagct gcctggtgat atcacagcct tctcctcagg cctcttttct tccaggcttt
      361 ggtgttaaga tgttgagtac tgccaacatg aagagcagtc agtacattcc gatgcatggc
      421 aaacagatcc gtggactggc gtttagcagt tacctcagag gcttgctact ctctgcttcc
      481 ctagacaaca ctattaaact gaccagcctg gagacaaata ccgtggtcca gacttataat
      541 gctggacgtc ctgtctggag ctgttgctgg tgtcttgatg aggctaacta catctatgct
      601 ggactggcca atggttcaat tctggtatat gacgtgcgaa acacgagcag tcatgtgcag
      661 gagttagtag ctcagaaagc cagatgccca ctggtctccc tgtcatacat gcccagagct
      721 gcctcagctg catttccata tggtggggtg ctggctggaa ccttggagga tgcttcattc
      781 tgggaacaga aaatggactt ttctcattgg cctcatgtgc tgcccttgga gccagggggc
      841 tgcatagact ttcagacaga gaacagctcc cggcactgtc ttgtgaccta caggcctgat
      901 aaaaatcaca ccaccatacg aagtgtgctg atggaaatgt cctaccgact ggatgacact
      961 ggaaatccaa tctgctcctg ccagcctgta catacatttt ttggaggacc tacttgcaaa
     1021 ctattgacca aaaatgccat tttccaaagc ccagagaatg atggcaacat cctggtgtgt
     1081 actggggatg aagcagcaaa ttctgccctg ctgtgggatg ctgccagtgg ctcgttgctc
     1141 caggacctac agaccgatca gcctgtgttg gacatctgcc catttgaggt gaaccgtaac
     1201 agctacttgg ctaccttaac agagaagatg gtccacatct ataagtggga gtgatgacga
     1261 agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta gaggttttac
     1321 ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg
     1381 ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     1441 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     1501 atgtatctta tcatgtctgg atctgatcac tgatatcgcc taggagatcc gaaccagata
     1561 agtgaaatct agttccaaac tattttgtca tttttaattt tcgtattagc ttacgacgct
     1621 acacccagtt cccatctatt ttgtcactct tccctaaata atccttaaaa actccatttc
     1681 cacccctccc agttcccaac tattttgtcc gcccacagcg gggcattttt cttcctgtta
     1741 tgtttttaat caaacatcct gccaactcca tgtgacaaac cgtcatcttc ggctactttt
     1801 tctctgtcac agaatgaaaa tttttctgtc atctcttcgt tattaatgtt tgtaattgac
     1861 tgaatatcaa cgcttatttg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     1921 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1981 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2041 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2101 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2161 tttcgccctt tgacgttgga gtccacgttc ttaatagtgg actcttgttc caaactggaa
     2221 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     2281 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     2341 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     2401 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     2461 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2521 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2581 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2641 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2701 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2761 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2821 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2881 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2941 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     3001 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     3061 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     3121 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     3181 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     3241 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     3301 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     3361 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     3421 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3481 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3541 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3601 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3661 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3721 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3781 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3841 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3901 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3961 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     4021 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     4081 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     4141 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     4201 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     4261 cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct
     4321 gtgcggtatt tcacaccgca tagaccagcc gcgtaacctg gcaaaatcgg ttacggttga
     4381 gtaataaatg gatgccctgc gtaagcgggt gtgggcggac aataaagtct taaactgaac
     4441 aaaatagatc taaactatga caataaagtc ttaaactaga cagaatagtt gtaaactgaa
     4501 atcagtccag ttatgctgtg aaaaagcata ctggactttt gttatggcta aagcaaactc
     4561 ttcattttct gaagtgcaaa ttgcccgtcg tattaaagag gggcgtggcc aagggcatgg
     4621 taaagactat attcgcggcg ttgtgacaat ttaccgaaca actccgcggc cgggaagccg
     4681 atctcggctt gaacgaattg ttaggtggcg gtacttgggt cgatatcaaa gtgcatcact
     4741 tcttcccgta tgcccaactt tgtatagaga gccactgcgg gatcgtcacc gtaatctgct
     4801 tgcacgtaga tcacataagc accaagcgcg ttggcctcat gcttgagcag attgatgagc
     4861 gcggtggcaa tgccctgcct ccggtgctcg ccggagactg cgagatcata gatatagatc
     4921 tcactacgcg gctgctcaaa cttgggcaga acgtaagccg cgagagcgcc aacaaccgct
     4981 tcttggtcga aggcagcaag cgcgatgaat gtcttactac ggagcaagtt cccgaggtaa
     5041 tcggagtccg gctgatgttg ggagtaggtg gctacgtctc cgaactcacg accgaaaaga
     5101 tcaagagcag cccgcatgga tttgacttgg tcagggccga gcctacatgt gcgaatgatg
     5161 cccatacttg agccacctaa ctttgtttta gggcgactgc cctgctgcgt aacatcgttg
     5221 ctgctccata acatcaaaca tcgacccacg gcgtaacgcg cttgctgctt ggatgcccga
     5281 ggcatagact gtacaaaaaa acagtcataa caagccatga aaaccgccac tgcgccgtta
     5341 ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg agcgcatacg ctacttgcat
     5401 tacagtttac gaaccgaaca ggcttatgtc aactgggttc gtgccttcat ccgtttccac
     5461 ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg aggcatttct gtcctggctg
     5521 gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg cattggcggc cttgctgttc
     5581 ttctacggca aggtgctgtg cacggatctg ccctggcttc aggagatcgg aagacctcgg
     5641 ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag tggttcgcat cctcggtttt
     5701 ctggaaggcg agcatcgttt gttcgcccag gactctagct atagttctag tggttggcta
     5761 cgtatactcc ggaatattaa tag
//
