LOCUS       sequence-232850-        9927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9927)
  AUTHORS   Reijmers LG, Perkins BL, Matsuo N, Mayford M
  TITLE     Localization of a stable neural correlate of associative memory.
  JOURNAL   Science. 2007 Aug 31;317(5842):1230-3.
  PUBMED    17761885
REFERENCE   2  (bases 1 to 9927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2007-08-31"; volume: "317(5842)"; pages: "1230-3"
COMMENT     Sequence Label: pTRE_tau-LacZ::tTA-H100Y
FEATURES             Location/Qualifiers
     source          1..9927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      1..10
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1165..4212
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     primer_bind     complement(1167..1189)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     promoter        complement(4268..4286)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     intron          4370..4435
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4565..4585
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4857..4991
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4894..4913)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4948..4967
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5421..5438)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5592..6180)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5672..5691)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6351..7142,7143..7211))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6974..6993
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7212..7316)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7342..7797)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7479..7500)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7691..7710
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    7929..8153
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8142..8159
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8179..8197
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     CDS             complement(8388..8411)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(8412..8432)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     protein_bind    join(9156..9174,9175..9197,9198..9216,9217..9239,9240..9258,
                     9259..9281,9282..9300,9301..9323,9324..9342,9343..9365,
                     9366..9384,9385..9407,9408..9426)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        9459..9497
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9494..9518
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          9652..9881
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     9864..9883
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
ORIGIN
        1 gccaccatgg ctgagccccg ccaggagttc gacgtgatgg aagatcatgc tcagggggac
       61 tacaccctgc aagaccagga gggtgacatg gaccccggcc tgaaagagtc tcccctgcag
      121 accccggccg atgatggatc tgaggaacca ggctctgaaa cctctgatgc taagagcact
      181 ccgacggcgg aagatgcgac agcaccctta gtggatgagg gagcccccgg tgagcaggcg
      241 gccgctcagg cccccgcgga gatcccagaa ggaaccgcag ctgaagaagc aggcattggc
      301 gacacgtcca acctggaaga ccaagctgcc ggacacgtga cccaagctcg catggtcagt
      361 aaaggcaaag atgggactgg acccgatgac aaaaaaacca agggggcgga tggtaagcct
      421 ggaacgaaga ttgccacacc ccggggagca gcccctccag gccagaaagg ccaggccaac
      481 gccacccgga ttccagcaaa aaccactccc accccgaaga cctcgccagg tgaatctggg
      541 aaatccgggg accgcagcgg ctacagcagc cccggctccc caggcactcc gggcagccgc
      601 tcccgcacac cctccctgcc gaccccgccc acccgggagc ccaagaaggt ggcggtggtc
      661 cgcactcccc ccaagtcgcc gtctgcagcc aagagccgcc tgcaggccgc tcccgggccc
      721 atgccagacc tgaagaacgt caagtccaaa atcggctcca cggaaaacct gaagcaccag
      781 ccaggaggtg gcaaggtgca gataattaat aagaagctgg atcttagcaa cgtccagtcc
      841 aagtgtggct caaaggataa tatcaaacac gtgccaggag gcggcagtgt gcaaatagtc
      901 tacaaaccag tggatctgag caaggtgacc tccaagtgtg gctcattagg caacatccat
      961 cataagccag gaggtggcca agtggaagtg aaatctgaga agctggactt caaggataga
     1021 gtccagtcga agattgggtc cctggacaac atcacacacg tccctggcgg agggaataaa
     1081 aagatcgaaa cccacaagct gaccttccgc gagaacgcca aagccaagac cgaccacggg
     1141 gcggagatcg tgtccgggga tcccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     1201 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     1261 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcttt
     1321 gcctggtttc cggcaccaga agcggtgccg gaaagctggc tggagtgcga tcttcctgag
     1381 gccgatactg tcgtcgtccc ctcaaactgg cagatgcacg gttacgatgc gcccatctac
     1441 accaacgtga cctatcccat tacggtcaat ccgccgtttg ttcccacgga gaatccgacg
     1501 ggttgttact cgctcacatt taatgttgat gaaagctggc tacaggaagg ccagacgcga
     1561 attatttttg atggcgttaa ctcggcgttt catctgtggt gcaacgggcg ctgggtcggt
     1621 tacggccagg acagtcgttt gccgtctgaa tttgacctga gcgcattttt acgcgccgga
     1681 gaaaaccgcc tcgcggtgat ggtgctgcgc tggagtgacg gcagttatct ggaagatcag
     1741 gatatgtggc ggatgagcgg cattttccgt gacgtctcgt tgctgcataa accgactaca
     1801 caaatcagcg atttccatgt tgccactcgc tttaatgatg atttcagccg cgctgtactg
     1861 gaggctgaag ttcagatgtg cggcgagttg cgtgactacc tacgggtaac agtttcttta
     1921 tggcagggtg aaacgcaggt cgccagcggc accgcgcctt tcggcggtga aattatcgat
     1981 gagcgtggtg gttatgccga tcgcgtcaca ctacgtctga acgtcgaaaa cccgaaactg
     2041 tggagcgccg aaatcccgaa tctctatcgt gcggtggttg aactgcacac cgccgacggc
     2101 acgctgattg aagcagaagc ctgcgatgtc ggtttccgcg aggtgcggat tgaaaatggt
     2161 ctgctgctgc tgaacggcaa gccgttgctg attcgaggcg ttaaccgtca cgagcatcat
     2221 cctctgcatg gtcaggtcat ggatgagcag acgatggtgc aggatatcct gctgatgaag
     2281 cagaacaact ttaacgccgt gcgctgttcg cattatccga accatccgct gtggtacacg
     2341 ctgtgcgacc gctacggcct gtatgtggtg gatgaagcca atattgaaac ccacggcatg
     2401 gtgccaatga atcgtctgac cgatgatccg cgctggctac cggcgatgag cgaacgcgta
     2461 acgcgaatgg tgcagcgcga tcgtaatcac ccgagtgtga tcatctggtc gctggggaat
     2521 gaatcaggcc acggcgctaa tcacgacgcg ctgtatcgct ggatcaaatc tgtcgatcct
     2581 tcccgcccgg tgcagtatga aggcggcgga gccgacacca cggccaccga tattatttgc
     2641 ccgatgtacg cgcgcgtgga tgaagaccag cccttcccgg ctgtgccgaa atggtccatc
     2701 aaaaaatggc tttcgctacc tggagagacg cgcccgctga tcctttgcga atacgcccac
     2761 gcgatgggta acagtcttgg cggtttcgct aaatactggc aggcgtttcg tcagtatccc
     2821 cgtttacagg gcggcttcgt ctgggactgg gtggatcagt cgctgattaa atatgatgaa
     2881 aacggcaacc cgtggtcggc ttacggcggt gattttggcg atacgccgaa cgatcgccag
     2941 ttctgtatga acggtctggt ctttgccgac cgcacgccgc atccagcgct gacggaagca
     3001 aaacaccagc agcagttttt ccagttccgt ttatccgggc aaaccatcga agtgaccagc
     3061 gaatacctgt tccgtcatag cgataacgag ctcctgcact ggatggtggc gctggatggt
     3121 aagccgctgg caagcggtga agtgcctctg gatgtcgctc cacaaggtaa acagttgatt
     3181 gaactgcctg aactaccgca gccggagagc gccgggcaac tctggctcac agtacgcgta
     3241 gtgcaaccga acgcgaccgc atggtcagaa gccgggcaca tcagcgcctg gcagcagtgg
     3301 cgtctggcgg aaaacctcag tgtgacgctc cccgccgcgt cccacgccat cccgcatctg
     3361 accaccagcg aaatggattt ttgcatcgag ctgggtaata agcgttggca atttaaccgc
     3421 cagtcaggct ttctttcaca gatgtggatt ggcgataaaa aacaactgct gacgccgctg
     3481 cgcgatcagt tcacccgtgc accgctggat aacgacattg gcgtaagtga agcgacccgc
     3541 attgacccta acgcctgggt cgaacgctgg aaggcggcgg gccattacca ggccgaagca
     3601 gcgttgttgc agtgcacggc agatacactt gctgatgcgg tgctgattac gaccgctcac
     3661 gcgtggcagc atcaggggaa aaccttattt atcagccgga aaacctaccg gattgatggt
     3721 agtggtcaaa tggcgattac cgttgatgtt gaagtggcga gcgatacacc gcatccggcg
     3781 cggattggcc tgaactgcca gctggcgcag gtagcagagc gggtaaactg gctcggatta
     3841 gggccgcaag aaaactatcc cgaccgcctt actgccgcct gttttgaccg ctgggatctg
     3901 ccattgtcag acatgtatac cccgtacgtc ttcccgagcg aaaacggtct gcgctgcggg
     3961 acgcgcgaat tgaattatgg cccacaccag tggcgcggcg acttccagtt caacatcagc
     4021 cgctacagtc aacagcaact gatggaaacc agccatcgcc atctgctgca cgcggaagaa
     4081 ggcacatggc tgaatatcga cggtttccat atggggattg gtggcgacga ctcctggagc
     4141 ccgtcagtat cggcggaatt acagctgagc gccggtcgct accattacca gttggtctgg
     4201 tgtcaaaaat aaatccatcc atcacactgg cggccggccg ctcgagcatg catctagagg
     4261 gccctattct atagtgtcac ctaaatgcta gaggatcttt gtgaaggaac cttacttctg
     4321 tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg taaatataaa
     4381 atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc
     4441 aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt
     4501 ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac
     4561 tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag
     4621 ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac
     4681 aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatatttga tgtatagtgc
     4741 cttgactaga gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     4801 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     4861 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     4921 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     4981 atgtctggat cgatcccgcc atggtatcaa cgccatattt ctatttacag tagggacctc
     5041 ttcgttgtgt aggtaccgct gtattcctag ggaaatagta gaggcacctt gaactgtctg
     5101 catcagccat atagcccccg ctgttcgatt tacaaacaca ggcacagtac tgacaaaccc
     5161 atacacctcc tctgaaatac ccatagttgc tagggctgtc tccgaactca ttacaccctc
     5221 caaagtcaga gctgtaattt cgccatcaag ggcagcgagg gcttctccag ataaaatagc
     5281 ttctgccgag agtcccgtaa gggtagacac ttcagctaat ccctcgagcg cggccgattt
     5341 aaatgtttaa accccctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     5401 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     5461 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     5521 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     5581 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     5641 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     5701 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     5761 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     5821 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5881 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5941 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     6001 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     6061 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     6121 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     6181 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     6241 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     6301 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     6361 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     6421 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     6481 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     6541 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     6601 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     6661 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     6721 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     6781 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6841 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6901 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6961 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     7021 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     7081 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     7141 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     7201 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     7261 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     7321 tttccccgaa aagtgccacc taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa
     7381 atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata
     7441 aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac
     7501 tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc
     7561 cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa
     7621 atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg
     7681 cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg
     7741 tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtccc
     7801 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     7861 tacgccagcc cgtttaaaca tttaaattcg aaccctcgag ccccagctgg ttctttccgc
     7921 ctcagaagcc atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc
     7981 ccccttgctg tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg
     8041 cgatgcaatt tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa
     8101 ggaaggcacg ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc
     8161 tgatcagcga gctctagcat ttaggtgaca ctatagaata gggcccaccg gttactagat
     8221 gcatgctcga gcggccggcc gccagtgtga tggatggatg atccttatca cagcatgtcc
     8281 aggtcgaagt cgtccagggc gtcggccggc agcatgtcca ggtcgaagtc gtccagggcg
     8341 tcggccggca gcatgtccag gtcgaagtcg tccagggcgt cggccggctt gtcgtcgtcg
     8401 tccttgtagt cgaccttgcg cttcttcttg ggggagccgg actcgcactt cagctgcttc
     8461 tccaggccgc agatgatcag ctccaggccg aacaggaagg cgggctcggc gccctggtgg
     8521 tcgaacagct cgatggcctg gcgcagcagg gggggcatgg agtcggtggt gggggtctcg
     8581 cgctcctcct tggccacctg gtgctcctgg tcctccagca cgcagcccag ggtgaagtgg
     8641 cccacggcgg acagggcgta cagggcgttc tccagggaga agccctgctg gcacaggaag
     8701 gccagctggt tctccagggt ctcgtactgc ttctcggtgg ggcgggtgcc caggtacacc
     8761 ttggcgccgt cgcggtggga cagcagtgcg cagcggaagg acttggcgtt gttgcgcagg
     8821 aagtcctgcc aggactcgcc ctccaggggg cagaagtggg tgtggtggcg gtccagcatc
     8881 tcgatggcca gggcgtccag cagggcgcgc ttgttcttca cgtgccagta cagggtgggc
     8941 tgctccacgc ccagcttctg ggccagcttg cgggtggtca ggccctcgat gcccacctcg
     9001 ttcagcagct ccagggcgga gttgatcacc ttggacttgt ccagtcgcga cataagctag
     9061 cgtgttcgag gccacacgcg tcaccttaat atgcgaagtg gacctgggac cgcgccgccc
     9121 gactgtacac ggccgccaac tctcgagttt accactccct atcagtgata gagaaaagtg
     9181 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9241 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     9301 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     9361 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     9421 tagagaaaag tgaaagtcga gctcggtacc cgggtcgagg taggcgtgta cggtgggagg
     9481 cctatataag cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct
     9541 gttttgacct ccatagaaga caccgggacc gatccagcct ccgcggcccc gaattcacca
     9601 cgtggccgca tcgattctag aattcgctgt ctgcgagggc cagctgttgg ggtgagtact
     9661 ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa aacgaggagg
     9721 atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc atctggtcag
     9781 aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc tggccataca
     9841 cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact cccaggtcca
     9901 actgcagccc aagcggatcc tgcggat
//
