LOCUS       sequence-232886-        3705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Encodes human full-length HDAC1 to be expressed in a 
            baculovirus/insect cell expression system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Inui K, Zhao Z, Yuan J, Jayaprakash S, Le LTM, Drakulic S, Sander B,
            Golas MM
  TITLE     Stepwise assembly of functional C-terminal REST/NRSF transcriptional
            repressor complexes as a drug target.
  JOURNAL   Protein Sci. 2017 May;26(5):997-1011. doi: 10.1002/pro.3142. Epub 
            2017 Mar 12.
  PUBMED    28218430
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1002/pro.3142"; 
            journalName: "Protein Sci"; date: "2017-05"; volume: "26"; issue: 
            "5"; pages: "997-1011"
COMMENT     Sequence Label: pIDK-HDAC1fl
FEATURES             Location/Qualifiers
     source          1..3705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        15..124
                     /label=p10 promoter
                     /note="baculovirus promoter for expression in insect cells"
     primer_bind     complement(1637..1656)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    1681..1729
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     protein_bind    1931..1964
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      1966..2322
                     /label=R6K gamma ori
                     /note="gamma replication origin from E. coli plasmid R6K; 
                     requires the R6K initiator protein pi for replication"
     CDS             2686..3480
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQLTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2740..2759)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3350..3369
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
ORIGIN
        1 gatactagta tacggacctt taattcaacc caacacaata tattatagtt aaataagaat
       61 tattatcaaa tcatttgtat attaattaaa atactatact gtaaattaca ttttatttac
      121 aatcactcga cgaagacttg atcacccggg atctcgagat ggcccagaca cagggcactc
      181 gtcgtaaagt ctgctactat tacgacggcg acgtcggcaa ctactactac ggtcagggcc
      241 accctatgaa acctcaccgc atccgtatga cccacaactt gctgttgaac tacggactct
      301 accgcaagat ggaaatctac cgtcctcaca aggccaacgc tgaggaaatg actaagtacc
      361 actctgacga ctacattaaa ttcttgcgct ccatccgtcc tgataacatg agcgaatact
      421 ctaaacagat gcagcgtttt aacgtgggag aagattgccc cgtgttcgac ggtctgtttg
      481 agttctgtca gctgagcaca ggcggcagcg ttgcctctgc tgtgaagctc aacaagcagc
      541 aaactgacat cgctgttaac tgggctggcg gtttgcacca cgccaagaag tctgaggctt
      601 caggcttctg ctacgtgaac gacatcgttc tcgccatctt ggagttgttg aagtaccatc
      661 aacgcgtcct gtacattgat atcgacattc accatggcga tggagttgag gaggcctttt
      721 acactacaga tagagtcatg actgtgagct tccacaagta tggcgaatac ttcccaggta
      781 caggtgacct ccgcgatatt ggtgctggta aaggcaagta ctacgctgtg aactacccac
      841 tcagggatgg aatcgacgac gaatcttacg aagctatctt caagcccgtc atgagcaaag
      901 ttatggaaat gttccaacca tctgctgttg tcctgcaatg cggcagtgat agcctgagcg
      961 gtgatcgcct cggttgcttc aacctgacca tcaagggaca cgccaaatgt gtcgagttcg
     1021 tgaagtcttt caacctgcct atgctgatgc tcggtggagg aggctacaca atccgcaacg
     1081 tcgcccgctg ttggacctat gagacagccg tggctctgga cactgagatc cccaacgagc
     1141 tgccatacaa cgactacttc gagtacttcg gcccagactt caagctgcac atctcaccct
     1201 caaacatgac aaatcaaaat accaacgagt acctcgaaaa gatcaagcaa cgtctcttcg
     1261 agaatctcag aatgctgcca catgcccctg gagtgcaaat gcaagctatc cctgaagacg
     1321 ccattcctga ggaatcagga gatgaggacg aagacgatcc cgacaagagg atctccatct
     1381 gttcctccga caagcgtatc gcttgtgaag aagagttttc cgacagtgaa gaggagggag
     1441 aaggaggcag gaagaattca tcaaacttca agaaggccaa gagggtgaag accgaagacg
     1501 agaaggaaaa ggaccccgag gaaaagaagg aagttaccga agaggaaaag accaaggaag
     1561 aaaagcctga ggccaagggt gtgaaggagg aggttaaact ggctctctaa ggtaccggga
     1621 gatgggggag gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga
     1681 cggcaataaa aagacagaat aaaacgcacg ggtgttgggt cgtttgttca taaacgcggg
     1741 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg ggaccaatac
     1801 gcccgcgttt cttccttttc cccaccccaa cccccaagtt cgggtgaagg cccagggctc
     1861 gcagccaacg tcggggcggc aagccctgcc atagccacta cgggtacgtt taaacccatg
     1921 tgcctggcag ataacttcgt ataatgtatg ctatacgaag ttatggtacg tactaagctc
     1981 tcatgtttca cgtactaagc tctcatgttt aacgtactaa gctctcatgt ttaacgaact
     2041 aaaccctcat ggctaacgta ctaagctctc atggctaacg tactaagctc tcatgtttca
     2101 cgtactaagc tctcatgttt gaacaataaa attaatataa atcagcaact taaatagcct
     2161 ctaaggtttt aagttttata agaaaaaaaa gaatatataa ggcttttaaa gcttttaagg
     2221 tttaacggtt gtggacaaca agccagggat gtaacgcact gagaagccct tagagcctct
     2281 caaagcaatt ttgagtgaca caggaacact taacggctga catgggaatt agcttcacgc
     2341 tgccgcaagc actcagggcg caagggctgc taaaggaagc ggaacacgta gaaagccagt
     2401 ccgcagaaac ggtgctgacc ccggatgaat gtcagctact gggctatctg gacaagggaa
     2461 aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc ttacatggcg atagctagac
     2521 tgggcggttt tatggacagc aagcgaaccg gaattgccag ctggggcgcc ctctggtaag
     2581 gttgggaagc cctgcaaagt aaactggatg gctttcttgc cgccaaggat ctgatggcgc
     2641 aggggatcaa gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
     2701 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
     2761 caactgacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
     2821 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg
     2881 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
     2941 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
     3001 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
     3061 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
     3121 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
     3181 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc
     3241 gtgacacatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
     3301 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
     3361 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
     3421 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
     3481 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
     3541 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
     3601 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggatct
     3661 atgtcgggtg cggagaaaga ggtaatgaaa tggcacctag gtatc
//
