LOCUS       sequence-232933-        9564 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the 10 N-terminal extracellular domains of the Dscam, 
            isoform 7.27.22 protein, C-terminally fused to alkaline phosphatase 
            + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9564)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9564)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Dscam1_7.27.22 EC10-AP-His
FEATURES             Location/Qualifiers
     source          1..9564
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        35..418
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        419..622
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     573..593
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     619..643
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          758..1583
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             6155..6172
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6198..6319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6235..6254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6289..6308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6357..6374)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6528..7116)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6608..6627)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7287..8078,8079..8147))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7910..7929
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8148..8252)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8320..8338
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8376..8398)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8400..8420)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8405..8734
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8585..8720
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8647..8666
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8871..8890
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8957..9412)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9094..9115)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9306..9325
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9538..9560
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     join(9552..9564,1..5)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     join(9553..9564,1..5)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ccagtgaatt gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta
       61 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
      121 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
      181 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
      241 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc
      301 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
      361 cttacgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      421 gatgcggttt tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc
      481 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
      541 tccaaaatgt cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg
      601 ggaggtctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc
      661 acgctgtttt gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg
      721 gtgcattgga acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta
      781 taggcacacc cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac
      841 ccccgcttcc atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac
      901 cattattgac cactccccta ttggtgacga tactttccat tactaatcca taacatggct
      961 ctttgccaca actatctcta ttggctatat gccaatactc tgtccttcag agactgacac
     1021 ggactctgta tttttacagg atggggtccc atttattatt tacaaattca catatacaac
     1081 aacgccgtcc cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc
     1141 tcgggtacgt gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag
     1201 ccctggtccc atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg
     1261 gaggccagac ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg
     1321 gcggtagggt atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat
     1381 ggaagactta aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa
     1441 gagtcagagg taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca
     1501 gtactcgttg ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc
     1561 ctttccatgg gtcttttctg cagaagcttg aattctcgag gatccgcggc cgctactgcc
     1621 aacttcacac aacccgctca gcggaaacac ggcatacgaa atgaatatgc ccaacgaacg
     1681 cctcaaatgg ctaatgctgt tcgcagctgt tgccctcatt gcttgtggta gtcagaccct
     1741 agctgccaat cccccagatg ccgaccaaaa aggacccgtc ttcctcaagg aacccaccaa
     1801 ccgcattgac ttctccaact ccacgggcgc agagatcgag tgcaaggcca gcggcaatcc
     1861 catgcccgag attatttgga tcaggagcga cggtaccgcc gtgggtgatg tgcccggatt
     1921 gcgtcagatc tcatccgacg gcaagctggt cttccctccc ttccgcgccg aggactaccg
     1981 ccaggaggtc catgcccagg tgtacgcctg cctggcccgc aaccagttcg gatccattat
     2041 ctcccgggac gtccatgtgc gagccgttgt caatcagttc tatgaggcgg agattatgac
     2101 cgagtatgtt atcaggggta atgctgccgt tctgaagtgt tccatcccgt cgttcgtggc
     2161 tgattttgtt cgagttgagt cctggatcga tgatgagggc aatgtcttgt ccttttcgga
     2221 caattacgat ggaaagtatc tggtattgcc ctctggagag ctgcacatcc gtgaggttgg
     2281 acccgaggac ggatacaaga gctaccagtg ccgaaccaaa catcgtctga ccggagaaac
     2341 ccgattaagt gccacaaaag gacgattggt catcacagaa cctgtgggca gtgttgcgcc
     2401 taagatcagc gtagaaaatc gtctgaaaaa tgcagaggct cgaattggtt ccaccttcac
     2461 cctcttttgc cctggtcaag cctatccagt acccttcttt agatggtaca agttcatcga
     2521 aggaacaacc cgtaaacaag ctgtggtgct aaatgaccgt gtgaagcagg tctctggtac
     2581 tctcattatt aaggacgcag tagtcgagga ctccggaaaa tacctgtgcg tggtgaacaa
     2641 ttccgtggga ggcgaaagcg ttgagactgt gcttaccgtc actgcccccc tatccgctaa
     2701 gatcgatcct cccacacaga cagttgactt cggacgcccc gccgtattca cttgccagta
     2761 cactggaaat cccatcaaga ccgttagctg gatgaaggac ggcaaggcta tcggacacag
     2821 cgaacccgtg ctgcgcatcg aatccgtgaa gaaggaggac aagggcatgt accagtgctt
     2881 cgtaagaaac gaccaggaat cggcggaggc gagtgctgag ctgaagctcg gaggccgttt
     2941 cgatcccccc gtcatccgac aggccttcca ggaagagacc atggaacccg gaccaagtgt
     3001 attcctcaag tgcgtcgccg gcggaaaccc cacgcccgaa atttcctggg aactcgacgg
     3061 caagaaaatc gccaacaacg atcgctatca ggtcggacag tatgtgacgg tcaacggaga
     3121 tgtggtttcc tatctgaaca taacctcggt ccacgccaac gatggcggtc tctacaagtg
     3181 catagccaag agcaaagtgg gcgtggccga acactcggcc aagttgaatg tatatggact
     3241 gccctacatc cggcaaatgg agaagaaggc aattgttgct ggagaaaccc tgattgtcac
     3301 atgtcctgta gctggatacc ccatcgattc gattgtctgg gagcgagata accgtgccct
     3361 gcccatcaac cgcaaacaga aggtcttccc caacggaacg ctgattatcg agaatgtgga
     3421 acggaactca gaccaagcga cctacacttg cgttgccaag aatcaggaag gatactctgc
     3481 tcgaggatct ctggaagtgc aagtcatggt tcccccgaaa atcacacctt tcacctttgg
     3541 cgaggatccc accaatgtgg aggactcagt ctctgtgact tgtttgatat ccagtggaga
     3601 cttacccatc gatatcgagt ggctatttaa tgacttcgga atcagctcgt attcaggaat
     3661 gactgtcctc agaggtggaa agagaaccag catgctgacc atagataatg tccatgctcg
     3721 tcatgccgga aagtatagtt gtcgcgcaaa gaaccacgca gcagccgtca actacactac
     3781 tgagttgatt gtgaatgtac ctcccagatg gatcctcgag cccaccgata aggccttcgc
     3841 ccagggatcc gatgccaagg ttgaatgcaa ggctgatggc ttccccaaac cccaagttac
     3901 atggaagaaa gcagttggcg acacccccgg agagtacaag gatctaaaga agagcgacaa
     3961 catccgcgtg gaagagggca ctctgcatgt cgacaacatc caaaagacca acgagggcta
     4021 ctatctgtgc gaggctatca atggaatagg ctccggcctc tcggctgtga ttatgatcag
     4081 cgttcaggcc cctccagagt tcacggagaa gctgcgcaac cagaccgccc gacgaggaga
     4141 acccgccgta ctccagtgcg aggcgaaggg cgagaagccc attggcatct tgtggaacat
     4201 gaacaacatg cgactggacc caaagaacga caatcggtac accattcgtg aggagatcct
     4261 ttccaccgga gttatgtcca gtctgtcgat caagcgcacc gagagatccg actccgccct
     4321 attcacctgt gtcgccacta atgcctttgg atccgatgac gccagcataa atatgattgt
     4381 ccaggaagtt ccggagatgc catatgcttt gaaggtactc gacaaatccg gacgttccgt
     4441 gcagctgagc tgggcgcaac cctacgatgg caactcccct ctggacaggt acatcattga
     4501 gtttaagcgt tctcgtgcct cctggagcga aattgatcgc gtcatcgtgc ccggacacac
     4561 cacagaagcc caagtgcaga agctcagccc cgccaccacc tacaacattc gcatcgtggc
     4621 cgaaaacgcc atcggcacat cccaatcctc tgaagcggtc actatcatca ctagtggatc
     4681 ttccggaatc atcccagttg aggaggagaa cccggacttc tggaaccgcg aggcagccga
     4741 ggccctgggt gccgccaaga agctgcagcc tgcacagaca gccgccaaga acctcatcat
     4801 cttcctgggc gatgggatgg gggtgtctac ggtgacagct gccaggatcc taaaagggca
     4861 gaagaaggac aaactggggc ctgagatacc cctggccatg gaccgcttcc catatgtggc
     4921 tctgtccaag acatacaatg tagacaaaca tgtgccagac agtggagcca cagccacggc
     4981 ctacctgtgc ggggtcaagg gcaacttcca gaccattggc ttgagtgcag ccgcccgctt
     5041 taaccagtgc aacacgacac gcggcaacga ggtcatctcc gtgatgaatc gggccaagaa
     5101 agcagggaag tcagtgggag tggtaaccac cacacgagtg cagcacgcct cgccagccgg
     5161 cacctacgcc cacacggtga accgcaactg gtactcggac gccgacgtgc ctgcctcggc
     5221 ccgccaggag gggtgccagg acatcgctac gcagctcatc tccaacatgg acattgacgt
     5281 gatcctaggt ggaggccgaa agtacatgtt tcgcatggga accccagacc ctgagtaccc
     5341 agatgactac agccaaggtg ggaccaggct ggacgggaag aatctggtgc aggaatggct
     5401 ggcgaagcgc cagggtgccc ggtatgtgtg gaaccgcact gagctcatgc aggcttccct
     5461 ggacccgtct gtgacccatc tcatgggtct ctttgagcct ggagacatga aatacgagat
     5521 ccaccgagac tccacactgg acccctccct gatggagatg acagaggctg ccctgcgcct
     5581 gctgagcagg aacccccgcg gcttcttcct cttcgtggag ggtggtcgca tcgaccatgg
     5641 tcatcatgaa agcagggctt accgggcact gactgagacg atcatgttcg acgacgccat
     5701 tgagagggcg ggccagctca ccagcgagga ggacacgctg agcctcgtca ctgccgacca
     5761 ctcccacgtc ttctccttcg gaggctaccc cctgcgaggg agctccatct tcgggctggc
     5821 ccctggcaag gcccgggaca ggaaggccta cacggtcctc ctatacggaa acggtccagg
     5881 ctatgtgctc aaggacggcg cccggccgga tgttaccgag agcgagagcg ggagccccga
     5941 gtatcggcag cagtcagcag tgcccctgga cgaagagacc cacgcaggcg aggacgtggc
     6001 ggtgttcgcg cgcggcccgc aggcgcacct ggttcacggc gtgcaggagc agaccttcat
     6061 agcgcacgtc atggccttcg ccgcctgcct ggagccctac accgcctgcg acctggcgcc
     6121 ccccgccggc accaccgacg ccgcgcaccc gggtcatcat caccatcacc attaatctag
     6181 agatcttatt aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6241 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6301 aactcatcaa tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg
     6361 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6421 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6481 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     6541 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6601 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6661 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6721 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6781 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6841 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6901 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6961 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     7021 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     7081 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     7141 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     7201 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     7261 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     7321 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7381 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7441 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7501 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7561 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7621 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7681 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7741 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7801 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7861 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7921 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7981 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     8041 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     8101 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     8161 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     8221 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     8281 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     8341 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8401 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     8461 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
     8521 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
     8581 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     8641 tttttttatt tatgcagagg ccgaggccgc ctctgcctct gagctattcc agaagtagtg
     8701 aggaggcttt tttggaggcc taggcttttg caaaaagctc ccgggagctt gtatatccat
     8761 tttcgtcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     8821 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     8881 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     8941 aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     9001 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     9061 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     9121 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     9181 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     9241 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     9301 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     9361 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtccattcgc
     9421 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
     9481 agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc
     9541 agtcacgacg ttgtaaaacg acgg
//
