LOCUS       sequence-23304-e         653 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Yueh A, Leung J, Bhattacharyya S, Perrone LA, de los Santos K, Pu 
            SY, Goff SP
  TITLE     Interaction of moloney murine leukemia virus capsid with Ubc9 and 
            PIASy mediates SUMO-1 addition required early in infection.
  JOURNAL   J Virol. 2006 Jan . 80(1):342-52.
  PUBMED    16352559
REFERENCE   2  (bases 1 to 653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2006 Jan . 80(1):342-52."
COMMENT     Sequence Label: pSG5-His-SUMO1
FEATURES             Location/Qualifiers
     source          1..653
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..19
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    420..541
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(457..476)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     511..530
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(575..592)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcatcaccat caccatcacg gatccccggg aatttccggt ggtggtggtg gaattctaga
       61 ctccatggct gaccaggagg caaaaccttc aactgaggac ttgggggata agaaggaagg
      121 tgaatatatt aaactcaaag tcattggaca ggatagcagt gagattcact tcaaagtgaa
      181 aatgacaaca catctcaaga aactcaaaga atcatactgt caaagacagg gtgttccaat
      241 gaattcactc aggtttctct ttgagggtca gagaattgct gataatcata ctccaaaaga
      301 actgggaatg gaggaagaag atgtgattga agtttatcag gaacaaacgg ggggtcattc
      361 aacagtttag atattgatcc atgcaatcac gaattcggat ccagatctta ttaaagcaga
      421 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
      481 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
      541 atcatgtctg gtcgactcta gactcttccg cttcctcgct cactgactcg ctgcgctcgg
      601 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg tta
//
