LOCUS       sequence-233225-        8551 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of a fusion protein of single chain Tet 
            repressor with the human RNF169 Ubiquitin binding domain and BFP 
            expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8551)
  AUTHORS   Bashir S, Dang T, Rossius J, Wolf J, Kuhn R
  TITLE     Enhancement of CRISPR-Cas9 induced precise gene editing by targeting
            histone H2A-K15 ubiquitination.
  JOURNAL   BMC Biotechnol. 2020 Oct 23;20(1):57. doi: 
            10.1186/s12896-020-00650-x.
  PUBMED    33097066
REFERENCE   2  (bases 1 to 8551)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2020-10-23"; volume: "20(1):57. doi"; pages: " 
            10.1186/s12896-020-00650-x"
COMMENT     Sequence Label: pCAG-TetR-RNF169UBD
FEATURES             Location/Qualifiers
     source          1..8551
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          88..1104
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1028..1050
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        1120..1138
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1146..1766
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             1845..2465
                     /codon_start=1
                     /gene="synthetic gene"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     CDS             2466..2486
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     promoter        2542..2560
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      2562..2571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2571..2594
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(2756..2772)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2792..3016
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     intron          3237..4175
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4132..4152
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4210..4219
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4216..4218,4219..4221,4222..4935)
                     /codon_start=1
                     /product="enhanced blue variant of GFP (Ai et al., 2007)"
                     /label=EBFP2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTTGKLPVPWPTLVTTLSHGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GTYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNFNSHNIYIMAVKQKNGIK
                     VNFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDSHYLSTQSVLSKDPNEKRDHMVLL
                     EFRTAAGITLGMDELYK"
     primer_bind     complement(4261..4282)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     polyA_signal    4966..5077
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5195..5212)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5195..5211)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5204..5226)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(5352..5807)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5489..5510)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5701..5720
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        5834..5938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5939..6007,6008..6799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6157..6176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6970..7558
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7459..7478
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7712..7729
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7846..7867
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7882..7887,7888..7905,7906..7912)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7920..7936
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7925..7947
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7944..7960
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7944..7960
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7980..8359
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 aatcagagcg gcgcgctccg aaagtttcct tttatggcga ggcggcggcg gcggcggccc
       61 tataaaaagc gaagcgcgcg gcgggcggga gtcgctgcgc gctgccttcg ccccgtgccc
      121 cgctccgccg ccgcctcgcg ccgcccgccc cggctctgac tgaccgcgtt actcccacag
      181 gtgagcgggc gggacggccc ttctcctccg ggctgtaatt agcgcttggt ttaatgacgg
      241 cttgtttctt ttctgtggct gcgtgaaagc cttgaggggc tccgggaggg ccctttgtgc
      301 ggggggagcg gctcgggggg tgcgtgcgtg tgtgtgtgcg tggggagcgc cgcgtgcggc
      361 tccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg cggggctttg tgcgctccgc
      421 agtgtgcgcg aggggagcgc ggccgggggc ggtgccccgc ggtgcggggg gggctgcgag
      481 gggaacaaag gctgcgtgcg gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg
      541 tcggtcgggc tgcaaccccc cctgcacccc cctccccgag ttgctgagca cggcccggct
      601 tcgggtgcgg ggctccgtac ggggcgtggc gcggggctcg ccgtgccggg cggggggtgg
      661 cggcaggtgg gggtgccggg cggggcgggg ccgcctcggg ccggggaggg ctcgggggag
      721 gggcgcggcg gcccccggag cgccggcggc tgtcgaggcg cggcgagccg cagccattgc
      781 cttttatggt aatcgtgcga gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag
      841 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc
      901 gccggcagga aggaaatggg cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt
      961 ctccctctcc agcctcgggg ctgtccgcgg ggggacggct gccttcgggg gggacggggc
     1021 agggcggggt tcggcttctg gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt
     1081 catgccttct tctttttcct acagatcctt aattaataat aatacgactc actatagggg
     1141 ccaccatgtc cagactggac aagagcaaag tcatcaactc tgccttggag ctcctgaatg
     1201 aagttggcat tgagggcttg accaccagga agctggccca gaagctgggt gtggagcagc
     1261 ctaccctgta ctggcatgtg aagaacaaga gggctctgct tgatgccctg gccattgaga
     1321 tgttggacag gcaccacacc cacttctgcc ctctggaagg ggagtcctgg caggacttcc
     1381 tgaggaacaa tgccaagagc ttcagatgtg ccttgctctc ccaccgggat ggtgccaaag
     1441 ttcacttggg caccaggcct acagagaagc agtatgagac cctggagaac cagctggcat
     1501 tcctgtgcca acaaggcttc tccctggaaa atgccttgta tgccctctct gctgtgggcc
     1561 acttcacctt gggctgtgtg ctggaggacc aggagcacca agttgccaag gaggagaggg
     1621 agacccccac cactgactcc atgccaccac tgctgcggca agctattgag ttgtttgacc
     1681 accaaggggc tgagcctgca ttcctttttg gcctggaact gatcatctgt ggcctggaaa
     1741 agcagctgaa atgtgagtct ggctctcacg tgggaggagg tggtggaggt ggaggtagcg
     1801 gtggaggagg tagtggaggt ggtggaagcg gaggaggtgg tagcatgtcc agactggaca
     1861 agagcaaagt catcaactct gccttggagc tcctgaatga agttggcatt gagggcttga
     1921 ccaccaggaa gctggcccag aagctgggtg tggagcagcc taccctgtac tggcatgtga
     1981 agaacaagag ggctctgctt gatgccctgg ccattgagat gttggacagg caccacaccc
     2041 acttctgccc tctggaaggg gagtcctggc aggacttcct gaggaacaat gccaagagct
     2101 tcagatgtgc cttgctctcc caccgggatg gtgccaaagt tcacttgggc accaggccta
     2161 cagagaagca gtatgagacc ctggagaacc agctggcatt cctgtgccaa caaggcttct
     2221 ccctggaaaa tgccttgtat gccctctctg ctgtgggcca cttcaccttg ggctgtgtgc
     2281 tggaggacca ggagcaccaa gttgccaagg aggagaggga gacccccacc actgactcca
     2341 tgccaccact gctgcggcaa gctattgagt tgtttgacca ccaaggggct gagcctgcat
     2401 tcctttttgg cctggaactg atcatctgtg gcctggaaaa gcagctgaaa tgtgagtctg
     2461 gctctcctaa gaaaaagagg aaggtgcacg gaggaggtgg aagcggagga ggaggaagcg
     2521 gaggaggagg tagccgatta ataatacgac tcactatagg ggccaccatg gactacaagg
     2581 atgacgacga taaaaagctt cagcaagagg aagaagaccg acagttggct ctgcagttgc
     2641 agcgcatgtt cgacaatgag aggcggactg tgagccggcg aaaaggaagt gtggatcagt
     2701 atctcctacg gtccagcaac atggccgggg ccaagtagac gcgtaaatga ttgcagatcc
     2761 actagttcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt
     2821 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     2881 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2941 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat
     3001 gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctcgag atccactagt
     3061 tctagcctcg aggctagagc ggccccggtg cctagagaag gtggcgcggg gtaaactggg
     3121 aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa
     3181 gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggtaa
     3241 gtgccgtgtg tggttcccgc gggcctggcc tctttacggg ttatggccct tgcgtgcctt
     3301 gaattacttc cacctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg
     3361 ggtgggagag ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg
     3421 cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg
     3481 ctgctttcga taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt
     3541 tctggcaaga tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg
     3601 gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc
     3661 tgcgagcgcg gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg
     3721 tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg
     3781 caccagttgc gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat
     3841 ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct
     3901 ttccgtcctc agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc
     3961 tcgattagtt ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg
     4021 cgatggagtt tccccacact gagtgggtgg agactgaagt taggccagct tggcacttga
     4081 tgtaattctc cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc
     4141 agacagtggt tcaaagtttt tttcttccat ttcaggtgtc gtgagcgatc gcatcgacag
     4201 tgtttaaacg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4261 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgagggg cgagggcgag
     4321 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4381 gtgccctggc ccaccctcgt gaccaccctg agccacggcg tgcagtgctt cgcccgctac
     4441 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4501 gagcgcacca tcttcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc
     4561 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggcg tcgacttcaa ggaggacggc
     4621 aacatcctgg ggcacaagct ggagtacaac ttcaacagcc acaacatcta tatcatggcc
     4681 gtcaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacgt ggaggacggc
     4741 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     4801 ctgcccgaca gccactacct gagcacccag tccgtgctga gcaaagaccc caacgagaag
     4861 cgcgatcaca tggtcctgct ggagttccgc accgccgccg ggatcactct cggcatggac
     4921 gagctgtaca agtgagaatt cctagagctc gctgatcagc ctcgactgtg ccttctagtt
     4981 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
     5041 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
     5101 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gagaatagca
     5161 ggcatgcgtt taaacattag tcgacagggc cgccactggc cgtcgtttta caacgtcgtg
     5221 actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc cctttcgcca
     5281 gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga
     5341 atggcgaatg gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     5401 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     5461 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     5521 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     5581 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     5641 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     5701 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     5761 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     5821 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     5881 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat
     5941 gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt
     6001 ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg
     6061 agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga
     6121 agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg
     6181 tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt
     6241 tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg
     6301 cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg
     6361 aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga
     6421 tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc
     6481 tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc
     6541 ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc
     6601 ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg
     6661 cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac
     6721 gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc
     6781 actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt
     6841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     6901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     6961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     7021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     7081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg
     7141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     7201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     7261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     7321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     7381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     7441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     7501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     7561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     7621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     7681 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     7741 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
     7801 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
     7861 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
     7921 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgaggcgc gccggattcg
     7981 acattgatta ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc
     8041 atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa
     8101 cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac
     8161 tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca
     8221 agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg
     8281 gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt
     8341 agtcatcgct attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc
     8401 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     8461 gggggcgggg gggggggggg ggcgcgcgcc rggsgggggg ggggggggsg gggggggggg
     8521 gggggsgrgg sggrgrggkg sggcggcagc c
//
