LOCUS       sequence-23333-e         651 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for N-terminal ECFP tag fusion with your gene 
            of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 651)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE5n
FEATURES             Location/Qualifiers
     source          1..651
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(132..134,135..137,138..647)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNI"
     primer_bind     complement(177..198)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(438..457)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gctactgggc caatatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg cttttttata atgccaactt tgtacaaaaa agcaggcttt aaaggaacca
      121 attcagtcga catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
      181 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
      241 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
      301 cctggcccac cctcgtgacc accctgacct ggggcgtgca gtgcttcagc cgctaccccg
      361 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
      421 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
      481 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
      541 tcctggggca caagctggag tacaactaca tcagccacaa cgtctatatc accgccgaca
      601 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcaag g
//
