LOCUS       sequence-23337-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  modified pENTR vector for C-terminal 6x MYC tag fusion with your 
            gene of interest.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Dubin MJ, Bowler C, Benvenuto G
  TITLE     A modified Gateway cloning strategy for overexpressing tagged 
            proteins in plants.
  JOURNAL   Plant Methods. 2008 Jan 22. 4(1):3.
  PUBMED    18211686
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Methods. 2008 Jan 22. 4(1):3."
COMMENT     Sequence Label: pE3c
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    8..107
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             174..203
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             213..242
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             252..281
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             291..320
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             330..359
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             393..422
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    complement(446..545)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cttnccggcc aatatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga
       61 taagcantgn ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa
      121 ttcagtcgac tggatccggt acagatttcg gacatgcggc cgcacaagct atggagcaaa
      181 agctcatttc tgaagaggac ttgaatgaaa tggagcaaaa gctcatttct gaagaggact
      241 tgaatgaaat ggagcaaaag ctcatttctg aagaggactt gaatgaaatg gagcaaaagc
      301 tcatttctga agaggacttg aatgaaatgg agcaaaagct catttctgaa gaggacttga
      361 atgaaatgga gagcttgggc gacctcacca tggagcaaaa gctcatttct gaagaggact
      421 tgaattcctg actcgagata tctagacccn gctttcttgt acaaagttgg cattataaga
      481 aagcantgnt tatcaatttg ttgcaacgaa caggtcacta tcnntcanaa taaaatcntt
      541 atttgccatc cagntgcngc tctngcccgt gnctcaaant ctctgatgtt acattgccca
      601 agatnaangt atatc
//
