LOCUS       sequence-23344-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Sharpe PL, Craig NL
  TITLE     Host proteins can stimulate Tn7 transposition: a novel role for the 
            ribosomal protein L29 and the acyl carrier protein.
  JOURNAL   EMBO J. 1998 Oct 1. 17(19):5822-31.
  PUBMED    9755182
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J. 
            1998 Oct 1. 17(19):5822-31."
COMMENT     Sequence Label: pPLS24
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             165..187
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    202..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(226..245)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(227..245)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(329..348)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        554..631
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     complement(651..670)
                     /label=LacI-R
                     /note="LacI, reverse primer"
ORIGIN
        1 tacntangcn agggcagacc taaggtagcg accaccttgc ggttgccata cacctcatcc
       61 aacagctgct taggactaac aatcccttga taaacgcctg cccgtgcaat agtgctataa
      121 atcagctcat tcgagtacgg aacaggaaag tttcccatgg tatatctcct tcttaaagtt
      181 aaacaaaatt atttctagag gggaattgtt atccgctcac aattccccta tagtgagtcg
      241 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc
      301 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat
      361 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc
      421 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg
      481 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag
      541 cgtcgagatc ccggacacca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg
      601 cccggaagag agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc
      661 agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt
      721 ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa
      781 ccgcgtggca caacaactgg cgggcaaaca gtcgttgctt gg
//
