LOCUS       sequence-23351-e         919 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Fei P, Wang W, Kim SH, Wang S, Burns TF, Sax JK, Buzzai M, Dicker 
            DT, McKenna WG, Bernhard EJ, El-Deiry WS
  TITLE     Bnip3L is induced by p53 under hypoxia, and its knockdown promotes 
            tumor growth.
  JOURNAL   Cancer Cell. 2004 Dec . 6(6):597-609.
  PUBMED    15607964
REFERENCE   2  (bases 1 to 919)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2004 Dec . 6(6):597-609."
COMMENT     Sequence Label: Bnip3L RNAi pSuper
FEATURES             Location/Qualifiers
     source          1..919
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..129)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(114..130)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(158..176)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(197..213)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(197..213)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(210..232)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    221..237
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(245..251,252..269,270..275))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    290..311
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(428..445)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(679..698)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttggattt gggaatctta taagttctgt atgagaccac agatccccca gttcctgggt
       61 ggagctattc aagagatagc tccacccagg aactgttttt ggaaaagctt atcgataccg
      121 tcgacctcga gggggggccc ggtaccagct tttgttccct ttagtgaggg ttaatttcga
      181 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
      241 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
      301 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
      361 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt
      421 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
      481 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
      541 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      601 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      661 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      721 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      781 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      841 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      901 atcgtcttga gtccaccgg
//
