LOCUS       sequence-233618-       13220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Making Tet3 conditional knock out.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13220)
  AUTHORS   Zviran A, Mor N, Rais Y, Gingold H, Peles S, Chomsky E, Viukov S, 
            Buenrostro JD, Scognamiglio R, Weinberger L, Manor YS, Krupalnik V, 
            Zerbib M, Hezroni H, Jaitin AD, Larastiaso D, Gilad S, Benjamin S, 
            Gafni O, Mousa A, Ayyash M, Sheban D, Bayerl J, Aguilera-Castrejon 
            A, Massarwa R, Maza I, Hanna S, Stelzer Y, Ulitsky I, Greenleaf WJ, 
            Tanay A, Trumpp A, Amit I, Pilpel Y, Novershtern N, Hanna JH
  TITLE     The Molecular and Functional Foundations of Conducive Somatic Cell 
            Reprogramming to Ground State Pluripotency
  JOURNAL   ssrn.3155731
REFERENCE   2  (bases 1 to 13220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "ssrn.3155731"
COMMENT     Sequence Label: mTet3 flox donor
FEATURES             Location/Qualifiers
     source          1..13220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6168..6186
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    complement(6195..6228)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(6236..6254)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(6456..6467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6805..6823
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    6832..6865
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     promoter        6874..7373
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6909..6932)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7306..7325
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     promoter        7390..7437
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7456..8259
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7519..7538)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     8129..8148
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     protein_bind    8382..8415
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     protein_bind    complement(8422..8455)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        complement(8463..8481)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        10263..10367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10368..10436,10437..11228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAVATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10586..10605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11399..11987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11888..11907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    12050..12184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12074..12093)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     12128..12147
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     intron          12245..12310
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(12387..12959)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
ORIGIN
        1 gtcgacttgc agggcagtga gcaggaaata gacattcacc atcacgggac cctggagcca
       61 atgacccttg tggcagctgg tcacccctgt cctatgtaca tgccagcagc aggcatcctg
      121 tactgtcagg aactgtgttc atcttacact aactgtgtgt gactcctggg taaaagttgt
      181 agatcctgga agggaggagg tgcagaaagt ctaacctgtt atccgacctc ccctcactcc
      241 tggcaccccc tcccccaggg ctttgactcc ttttttctat gtgtctgagt ggccagtact
      301 cctgtcccct acctggaagc cagggaagac actgagggtg acctacaaga gccaggctgg
      361 ggacaagaca aggcctctgg atagtattat gctgaggctc gattcttccc agcctctaag
      421 cagagctctt gagtaagctg gtcagggaga aagtcattga cagacctcag tccctcagcg
      481 ttgccctttg gggcctcctc aagctcttga catcatgggt gtctgcagct acaaagctgg
      541 cttagagaga gctaggctct tgagaactgg gccacgttta atgccctagg caccctggag
      601 tcgagagctg gagacacaaa gtttaaccaa gagagggctg tgatgccctt tgtaggcgtt
      661 gaaggacggg ggctggatga atgttgaggc tgggctaccc tggaacacag acatgaacag
      721 gagaagaagc atttccgggg aggagggcct cacgggcaaa ggcaggaagg ccgcaggagt
      781 ttcatgtagc tgacctgaat gttgcctgca cgcttgccgt cttcccgtgg gatcactcct
      841 tgcctctgca tctctgcggt taatgaaaat aacacgactg aactcaaaac atcacacttg
      901 ttctcggcac ttctagccat tattgtagga cttttcacaa caaaggaacc tggtgctttt
      961 ttagaaagtg tgactgtgca tgttgtgtcc accccatggg agggggccct tgtagtaaaa
     1021 ggtctgatgt ggtaggaggt gcctcttcag agaacccacc tgcaaatgat gtcagtcata
     1081 agcagagttt gactgtaagg cctggacaac cctggctcac tgtcatagca gcttctgcag
     1141 atgtgtgtca gggcccacag taggcctcct acaatctgcc gggagccagg caaccagtca
     1201 gagtcttgaa gcagcatttg actcagcacc tcggttgtat gcttctgaat tccaagacac
     1261 atttcaaaaa taatagttga ttgttggcag gaaattggga gaataacatc attgtgaccc
     1321 tgccttagac caagacctca tgatcagggc tgtgtgccgt ttagcatgta aaataattct
     1381 gtaacatttg cagcaagtca cgaaacctgc tataacaagc agaacacctt gagagcactg
     1441 ccagttgtca actatagatt gcatttgatt ccacatccat gacctgcatc agagtcgggt
     1501 gacagaggct gaaaggacag tgctgaggga tgggaataga gagagctcac caaggatgta
     1561 caagacccca gatacagctt tcagcccagc taaatcaaat acaaactctg cagtcagcaa
     1621 gatggatggc tcagtggatg aaggcacttg tacacaggcc ttgtggcctg aattgcattc
     1681 ctacatctta cgaggtgtca gggcagaact gtctgctaag ttaccctttg gcctgtgtgg
     1741 ggcacaggca gagtaaataa aaggaaaatc ttaattagaa aaagcagaag aattatctca
     1801 ggcatcgggg agcatcagaa gcacgaataa taatccatga gtggagcttc ggcttattca
     1861 tttattggaa ggaaaagttc tagaccagtg gttctcaacc tgtgggtcct gacccctttg
     1921 gagggtcgca taccagttat cctgcatatc ggatatttac atattcaagc agtagtgaaa
     1981 atacagttat aaagtagcaa caaaaataat gttacgattg gggacaccac aacacgagga
     2041 actgaattaa agggacacag tgttaggaag gggctctaga ggacgtggta gcttcatgct
     2101 gaatgaacct aaagcctaca gtgcacactg tgtcagttta ttatgcatac tttaccgtag
     2161 taacgaagag tgtgtctcaa agaaagggca gggccacaga gtattataaa gctctccatt
     2221 tctagtcttt cttacaaact ggagaagcta gttgcacggg tcgaagtgcc ctgtgtggtt
     2281 aaggagagct aggcatctca gcttccaatg aacagatgtc cccattgagc cctaactttg
     2341 tcaggccccc tttgcttctt ggcattgcca acctagccac acctttctgt gtaacctgca
     2401 gagtgtggat ggcaggtgtg actacatctg tctgcagcct aggtccttcc tgactgccac
     2461 acctatgggt catactttgg aggagcctca ggctggtttg cacagtgtta tacccttgcg
     2521 tgtaaacaca gatgtgcagc actttctctg cccaagtgta aaacgtccat agctatgcaa
     2581 cgccttctct gcagacatgc tgggacatca taaaactctt cactatttct ggttcacact
     2641 tccctccttt taagtctgtg ttggtcacaa atgtcttaga cttcattgtc actacttttt
     2701 attttgctgt tttaaaatgt gtttcttctt ccctaatggt gctgtcctaa agatggacag
     2761 ctacaggtct gtgggatagt gaggggcatg taccatgcag agctgtgtgg ccctgcaggg
     2821 cagtggctca gtcccatgtc tctgcaggtg atccgaagac acacactgga ggagaagctg
     2881 ctgtgcctgg tgcggcatcg ggcaggccac cattgtcaga acgccgtgat tgttatcttg
     2941 atcctggcct gggagggcat ccctcgaagc cttggggaca ccctctacca ggagcttact
     3001 gataccctcc ggaagtatgg caaccctacc agccggagat gtggcctcaa tgatgagtat
     3061 gtattggagt ggtggaggtg gtgatggggc tgtagaacca gggtagcagg cactgggatg
     3121 ctgggcagtg ctgggttctg gccctggctc aactttactg ctcagcccca gatcaacact
     3181 aacctatctt tattagattt tcctaccctt tcattgagat gcaattagtt tcagtaaagt
     3241 taccctttcg gcatatagct tggaggattt tgttttattt ttgttgagac aaggtcttgc
     3301 tgtcctggaa ctagctatgt aaaccaagct agccttgatt tcaaagccta cctctgcctc
     3361 tggagtgcta agattaaagt catgctccac cacccccagc agcttagagg attttgatat
     3421 agtcatatta cccagtcgag attgagaaca ggggtccatt cgatggctca gtgggcaaag
     3481 gcaattgcca ccatgcctaa caactttagt tcattctcca ggaccaatat gataaaagag
     3541 aataagtcct gaagttgatg tccacataca catgtgcaca catgtgcaca tacgtgcaag
     3601 catgcataac tcttgtaccc ttctacaacc cttcccccag ccctcaacaa ccatgtgact
     3661 ttcctgaccc tttgagtttt ccctggagaa tgtttctttc caactcatat acattatgta
     3721 tccttctgaa caggcatgtt tcaccaagtg ttcttgttct attctgagtg ttcagagaat
     3781 aacctgtact gggaaacttg gaggaattgt ggggtggaag gaggagccct tgttgccaga
     3841 ggctagtgga aaccagtcct ctacaggggc agtgtcaaca gagaccacat ctgtatgctt
     3901 ggcctatgtt atccattgca gccggacctg tgcttgccaa ggcaaagacc ctaacacctg
     3961 cggtgcctcc ttctccttcg gctgttcctg gagcatgtac ttcaacggct gcaaatatgc
     4021 tcggagcaag acgccacgaa agttccgcct cacgggagac aatccgaagg aggtgagaaa
     4081 agcagtgccc ggcacacggc aggcccccct cgcgcctccc cacctgcgtc cttaacagct
     4141 atgcagagga aggattgaca tggcgctgca cagatggaca ggaaatgtat gacgttagca
     4201 gcccgtgtga aaactgaggg ctgctggcca caggtcctca gacctaacca cttacgtggc
     4261 agctttagag gaattattct gaatacctta gatacagatg cagatttttc atctgaattt
     4321 gatctggtca tttctttgcg gggcatcttt gcggggtgta ggaataggaa gaaggggggg
     4381 tcttcctctc tgtggatgcc ctctgaatct gtacagggct tggtaaggaa tcccatcccc
     4441 cactgggctg ggcagacatt gtgggcattc atacagacat ctaggccctt actgtctcca
     4501 tgtcctggtg cactgagtgc agtccacacg tacagtcact gggttgtgag tagccatggc
     4561 tactcatcct ggtctccctt caggacctct cttatgtccc tgtcacaacc gaacaagggc
     4621 ctctgggact cttgtagctt ccacaagggg tataggatct gtgtcagcca aggccatttc
     4681 tctattgctg tgttttcttg tgctccgtgc tacagtggcc atagcaagag gtggtctccg
     4741 aataaacaca gtgtcctttt caccccttct tcctccctcc tgcttgtact tccggggtgt
     4801 aggggtcagg ggtctaaccc ctctgctagg gtgcaagtat cctggccatc ctgatgccag
     4861 tcttggcctt ccagagctcg aggctcctag tactgattcc cctctgccca ggtcagggac
     4921 agccactggg ctcctcttct gtgcaccata gcccatgtaa ccattttcct ggctaaatag
     4981 ttttcaggga ctagagcact gctctcccaa atgacctgag ttcatgacct aggacccaca
     5041 gtgtggctca cagccatctg taactccagc ttaggggaac tgacaccttc ttctggcttc
     5101 tgcagccccc ccccctgcac atatatgcca taaagcatac acatataaaa atcttcattt
     5161 tcccaaaaaa cttttagaca attttgtaag ctaaaaatac tctagagctc tttcttctga
     5221 gcagcaaatg tggttgtctc agtgggttga agagtcccag ggccttagac tcgccagaat
     5281 aaaaggcaat ggctccattt ctccaagagc tgtccctggt gctgtagatt gccatctaca
     5341 cacggctcct tacccccacg tgaccaaccg gagagaccac tcagaacagt tgatggtgaa
     5401 ggaggcagga tccctcccca ggcctgtgct tccccagctc gagcctcacc ttggagctgt
     5461 gttacggcat catccagaca gtggcatagc agtggccagg ccccacacag catcgtgttg
     5521 gttgtactga ctggtgactg agccatgctc ctggcttctc tccatgctgt gtgcttgcta
     5581 tcactccttc aaaaatgctc catgtttgtg ttccacacct attggaaaat ccccgcccac
     5641 catactacac cagtgaccca cagtattcag ctctgagtgc aagaccctgc tctcttgtcc
     5701 tgggctgaac ggggctgtgt gtgactgatg gagtagctta gctgtcagga tgactgcacc
     5761 ttccaatagt ggcttttcct gtgagcctca gtatccccat tgaaaatgga cacaccaggg
     5821 gaacgctgag accctggacg cacttggctt cctgtcttcc cagcaatggt tctggtgggc
     5881 tcatttctgg caggaagttt gccggcttga gcagctctga atgtacctaa ttgtttgttc
     5941 ttcctgcagg aggaggtgct ccggaatagc tttcaggatc tggccactga agttgctccc
     6001 ctctacaagc ggctcgcacc ccaggcctat cagaaccagg tactgaagcc aagggccttt
     6061 gtcatgtgca gtttttactt aagttccctg acgttggaga gttgggagta ttgtttcctt
     6121 tgtgttttta aagtgcgttc aacctagagg tctctgatat cagtactaat taaccctcac
     6181 taaagggcgg ccgcataact tcgtatagca tacattatac gaagttatct cgagccctat
     6241 agtgagtcgt attatggagc cattgtgtgc tttgataaga ctccttcctt agtcacccct
     6301 agctcccctc ccccccttcc cctccccagt ggtctgcagt gcctctggcc tagcttcctc
     6361 tgaggagctt gagttcccat ctatccaggc agacaagctt gcgctaacct aggagagagc
     6421 cattccttca gagacaggac ctctcaactg gaaagccttc cctcaatggc ccagggtcac
     6481 acgacagctc tgatcttaac ctgctcctct ctaagatctg gatcccaggg gcagcgtcgg
     6541 ggctggtcac tcaggccttc tgaaccactg ttcttggcca ttcacacagg tgaccaatga
     6601 ggatgtggcg atcgactgcc gcctggggct gaaggaaggg agacccttct caggggtcac
     6661 agcctgcatg gacttctgtg cccacgccca caaggaccaa cataacctct acaatgggtg
     6721 cactgtggta aggaagcctg ggaccagcgg aaggagccaa ctaatgggtt ttgctacttg
     6781 tgaggaggga agtagagtgg attcaattaa ccctcactaa agggcggccg cgaagttcct
     6841 attctctaaa aagtatagga acttcattct accgggtagg ggaggcgctt ttcccaaggc
     6901 agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca agtggcctct
     6961 ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc tttggtggcc
     7021 ccttcgcgcc accttccact cctcccctag tcaggaagtt cccccccgcc ccgcagctcg
     7081 cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg cagatggaca
     7141 gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag cagctttgct
     7201 ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg ctcaggggcg
     7261 ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc tgcacgcttc
     7321 aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt tcgacctgca
     7381 gcagcacgtg ttgacaatta atcatcggca tagtatatcg gcatagtata atacgacaag
     7441 gtgaggaact aaaccatggg atcggccatt gaacaagatg gattgcacgc aggttctccg
     7501 gccgcttggg tggagaggct attcggctat gactgggcac aacagacgat cggctgctct
     7561 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
     7621 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
     7681 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
     7741 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
     7801 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
     7861 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
     7921 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
     7981 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
     8041 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
     8101 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
     8161 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
     8221 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcaaat
     8281 aaagaccgac caagcgacgt ctgagagctc cctggcgaat tcggtaccaa taaaagagct
     8341 ttattttcat gatctgtgtg ttggtttttg tgtgcggcgc ggaagttcct attctctaga
     8401 aagtatagga acttcaagct tataacttcg tatagcatac attatacgaa gttatctcga
     8461 gccctatagt gagtcgtatt attgaagccc taggtagtgt tggcttagat ggcctgctcc
     8521 tgcccttcca cagagataac cctcagatca gtgacaggag gcatgcgagg aaccagagcc
     8581 aacgtgtgaa ttcactgagc agtagatgag gtttgggata aaaaatggaa gtcttcctag
     8641 agatgcctgc taggctacat ggttgagtaa gagcaggcag ggaggtttga atgagtgggt
     8701 ctgcatgcgt gtgctggaga agacggggag tcggggctga ctgaggtgga gacaccctca
     8761 agggaacggg tgctgctgtg ggcggctgtg cttgcttttc agctgggctc agtacagcct
     8821 ggactgcagg ggctgaggag aaggctgggg tcccagactg aggtgctagc aagggcaagt
     8881 cctcaaggat agagggggca agaggtggtg acactggggg agccggcaga agcaagagca
     8941 tggggaggcc acctgctctc cacactcctg cacgctttac tattaaagat taactgtcag
     9001 cagccaaact ctcaaacttc tcatgtctta gtctctgcgt gcctgtacac agctgggtct
     9061 ctgcatgtgg acagccgtgt gtgcgtgcac atgcatgcat gtgaatgtgc ctggaggaca
     9121 aaggtcgatc tctggtgctc ctgtaaagct gtccatccct aaaggtttta gtgtcgtttt
     9181 tgagacagtc tctcattggc acctggagtt aggctgggta gccagtaaga tgaggtccac
     9241 ttgtgcttgg cttcttctgt gggtcctgga gatgtaactc gggccctcca gttggcacag
     9301 tgatagcttt acacactgag ccatctgctt tttttttttt ttttttttta gtatagagtt
     9361 tattcagggc atggggagag gagttaagag ggtagtagag aagagaaagg cagagagaaa
     9421 gagagagtag agaagtagag gccggccatg gggagagagg gggaggcaag agagagctga
     9481 gccatctgct tataggaatt tggagagttg ggaactggag agctggctta gcagttgaga
     9541 gcactgctac ccttcaggag acagaccctc agtttatttc tcagcaaacc ctttcaggtg
     9601 tcgccaccat cagctttagg gaatctggtg ccctcttcta gcctttgtga gacatacatc
     9661 atatattcac atggatataa acacatacat agaattaaaa aaaaaaacgt ttaaaaaccg
     9721 ttttctgaag tctttagaga tagccacagt cagcatttgg tggccacccc ttctacggct
     9781 gtgtgtatga ccatggccac atagctgtat aaaactctta tctacatggg ggtcagagtc
     9841 tacctgctgt cctgtagtgt gcttttccta gccagtggta acttgggctt tctgtgtcag
     9901 tgagtatgga ttcacattta aatttgttgg agcttcatgt gtagctctga tatcagctat
     9961 ttatttagcc agttccccca cagatgatgg gcagttggtg agtcacactc tctccatcca
    10021 tatgccatga ggaatcccta cagctcaggg cagaaatagt ttcctctgga gcgcccaccc
    10081 cctccccata gagatggtga cagtcacagg cttttagtgc ttgttcagtt tcctgcagta
    10141 cagggtcctg tcaggcggcc taattcagtg aggtgggcct cttattcgct gaagaggttg
    10201 aacatcttcc cctatttctc ttgcctctcc cgacgtcagg tggcactttt cggggaaatg
    10261 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
    10321 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
    10381 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
    10441 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
    10501 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
    10561 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
    10621 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
    10681 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
    10741 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
    10801 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
    10861 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcagtgg
    10921 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
    10981 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
    11041 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
    11101 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
    11161 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
    11221 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
    11281 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
    11341 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
    11401 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
    11461 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
    11521 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
    11581 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
    11641 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
    11701 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
    11761 gcaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
    11821 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
    11881 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
    11941 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
    12001 cggccttttt acggttcctg gccttttgca cggtccgctc tagaactacg atccagacat
    12061 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
    12121 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
    12181 agttagatcc tagtggatct gcattccacc actgctccca ttcatcagtt ccataggttg
    12241 gaatctaaaa tacacaaaca attagaatca gtagtttaac acattataca cttaaaaatt
    12301 ttatatttac cttaaagctt taaatctctg taggtagttt gtccaattat gtcacaccac
    12361 agaagtaagg ttccttcaca aagagatcgc ctgacacgat ttcctgcaca ggcttgagcc
    12421 atatactcat acatcgcatc ttggccacgt tttccacggg tttcaaaatt aatctcaagt
    12481 tctacgctta acgctttcgc ctgttcccag ttattaatat attcaacgct agaactcccc
    12541 tcagcgaagg gaaggctgag cactacacgc gaagcaccat caccgaacct tttgataaac
    12601 tcttccgttc cgacttgctc catcaacggt tcagtgagac ttaaacctaa ctctttctta
    12661 atagtttcgg cattatccac ttttagtgcg agaaccttcg tcagtcctgg atacgtcact
    12721 ttgaccacgc ctccagcttt tccagagagc gggttttcat tatctacaga gtatcccgca
    12781 gcgtcgtatt tattgtcggt actataaaac cctttccaat catcgtcata atttccttgt
    12841 gtaccagatt ttggcttttg tatacctttt tgaatggaat ctacataacc aggtttagtc
    12901 ccgtggtacg aagaaaagtt ttccatcaca aaagatttag aagaatcaac aacatcatca
    12961 gggtccatgg cgaggacctg caggtcgcag atcctgaacg gcagaggtta cggcagcttg
    13021 tctctccccc ttccgggagc caccttcttc tccaaccgtc ccggtcgcgc tctcggcgct
    13081 tctgaggaga gaactggctg agtgacgccc tttatagatt cgcccttgtg tcccgcccct
    13141 tcctttcccg ccctcccttg cgctacgggg ccgcccgcac cagcctacac ggagcgcgcg
    13201 cggcggagtt gttcggtccg
//
