LOCUS       sequence-233656-        1732 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector for expression of AcrIIA4 with two miR-122 binding sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1732)
  AUTHORS   Hoffmann MD, Aschenbrenner S, Grosse S, Rapti K, Domenger C, Fakhiri
            J, Mastel M, Borner K, Eils R, Grimm D, Niopek D
  TITLE     Cell-specific CRISPR-Cas9 activation by microRNA-dependent 
            expression of anti-CRISPR proteins.
  JOURNAL   Nucleic Acids Res. 2019 Apr 15. pii: 5458120. doi: 
            10.1093/nar/gkz271.
  PUBMED    30982889
REFERENCE   2  (bases 1 to 1732)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Apr 15. pii: 5458120. doi: 10.1093/nar/gkz271."
COMMENT     Sequence Label: AAV CMV-driven AcrIIA4-2xmiR-122 target sites
FEATURES             Location/Qualifiers
     source          1..1732
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        1..249
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1..21
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     172..191
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     enhancer        397..776
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        777..980
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     931..951
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     1025..1044
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1025..1043
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1065..1088
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(1502..1519)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1508..1732
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
       61 ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
      121 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
      181 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
      241 cgaaacaccg agtcttcgag gaagacaggt ttaagagcta tgctggaaac agcatagcaa
      301 gtttaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
      361 tgcggccgcg gccgctctac aaatgtggta tctagagaca ttgattattg actagttatt
      421 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      481 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      541 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      601 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      661 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      721 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      781 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      841 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      901 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      961 aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg
     1021 aaattaatac gactcactat agggagaccc aagctggcta gatggactat aaggacgatg
     1081 acgataaagc tggcggcagc atgaacatta acgacctgat tcgggaaata aagaacaagg
     1141 actacacggt caagctgtca ggcaccgact ccaactctat aacacagctc attataaggg
     1201 ttaataatga tggcaacgaa tatgtcatct ctgaatccga gaatgaatca atcgtggaga
     1261 aattcatcag tgcatttaaa aacggctgga accaggagta cgaagatgag gaagagttct
     1321 acaatgatat gcaaactatc acactgaaat ctgagctgaa ctagctcgag cggccgccac
     1381 tgtgctggat atctgcagaa ttcaaacacc attgtcacac tccattatat caaacaccat
     1441 tgtcacactc cattaattag agacgagctc gtctctagct taagtttaaa ccgctgatca
     1501 gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc
     1561 ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg
     1621 cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg
     1681 gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat gg
//
