LOCUS       sequence-233677-       10770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  conditional expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10770)
  AUTHORS   Connelly KE, Weaver TM, Alpsoy A, Gu BX, Musselman CA, Dykhuizen EC
  TITLE     Engagement of DNA and H3K27me3 by the CBX8 chromodomain drives 
            chromatin association.
  JOURNAL   Nucleic Acids Res. 2018 Dec 29. pii: 5266947. doi: 
            10.1093/nar/gky1290.
  PUBMED    30597065
REFERENCE   2  (bases 1 to 10770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2018 Dec 29. pii: 5266947. doi: 10.1093/nar/gky1290."
COMMENT     Sequence Label: Fuw CBX8 R20A
FEATURES             Location/Qualifiers
     source          1..10770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        48..427
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        429..627
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             645..825
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    872..997
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1490..1723
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1908..1952
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2250..2367
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    join(2424..2442,2443..2465,2466..2484,2485..2507,2508..2526,
                     2527..2549,2550..2568,2569..2591,2592..2610,2611..2633,
                     2634..2652,2653..2675,2676..2694)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     promoter        2727..2765
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2762..2786
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2883..2892
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2892..2933
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     promoter        4129..4628
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4164..4187)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4561..4580
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4649..5248
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4649..4668)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5145..5165
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5272..5860
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5325..5345)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5743..5754)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5863..5879)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5864..5880)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6385..6565
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6591..6608)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6597..6821
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6867..7295
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6954..6973)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7164..7185
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7304..7324)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7309..7638
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7489..7624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7551..7570
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7686..7733
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7752..8126
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8256..8377
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8293..8312)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8347..8366
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8426..8442)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8426..8442)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8439..8461)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8450..8466
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8474..8480,8481..8498,8499..8504))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8519..8540
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8657..8674)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8828..9416)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8908..8927)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9587..10378,10379..10447))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10210..10229
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10448..10552)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10627..10646)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac attgattatt
       61 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      121 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      181 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      241 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
      301 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      361 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      421 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      481 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      541 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      601 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      661 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      721 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      781 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      841 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      901 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      961 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     1021 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     1081 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     1141 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     1201 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1261 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1321 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1381 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1441 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1501 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1561 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1621 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1681 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1741 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1801 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1861 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1921 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1981 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     2041 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     2101 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     2161 agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt gcgccaattc
     2221 tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac
     2281 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
     2341 aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag agatccagtt
     2401 tggttagatc tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2461 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2521 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     2581 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     2641 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     2701 aaagtcgagc tcggtacccg ggtcgaggta ggcgtgtacg gtgggaggcc tatataagca
     2761 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
     2821 atagaagaca ccgggaccga tccagcctcc gcggccccga attgctagcc gatggatccg
     2881 tggccaccat gggtaagcct atccctaacc ctctcctcgg tctcgattct acgggcggag
     2941 agctttcagc ggtgggggag cgggtgttcg cggccgaagc cctcctgaag cgggccatac
     3001 ggaaaggacg catggaatac ctcgtgaaat ggaagggatg gtcgcagaag tacagcacat
     3061 gggaaccgga ggaaaacatc ctggatgctc gcttgctcgc agcctttgag gaaagggaaa
     3121 gagagatgga gctctatggc cccaaaaagc gtggacccaa gcccaaaacc ttcctcctca
     3181 aagcgcaggc caaggcaaag gccaaaactt acgagtttcg aagtgactca gccaggggca
     3241 tccggatccc ctaccctggc cgctcgcccc aggacctggc ctccacttcc cgggcccggg
     3301 agggccttcg aaacatgggt ttgtccccgc cagcgagcag caccagcacc agcagcacct
     3361 gccgcgcaga ggcccctcgg gaccgggacc gagaccggga tagggaccgg gagcgggatc
     3421 gagaaaggga gagggagcga gagagggagc gggaacgtga gagggaacga gagcggggta
     3481 ccagcagagt ggatgacaag cccagctcac cgggggacag ctcgaagaag cgaggcccca
     3541 agccccggaa ggagctcccg gacccctcac agaggccctt aggcgaaccc agcgccggcc
     3601 tcggagagta cctcaagggc aggaagctgg acgacacccc ttccggggca ggaaagtttc
     3661 cagccggcca cagtgtgatc cagctggccc gaagacagga ctcggacctg gtgcagtgtg
     3721 gtgtgaccag ccctagctca gctgaggcca cgggcaaact ggctgtggac accttcccgg
     3781 ccagggtgat aaagcacagg gctgccttcc tggaggccaa aggccagggt gccctagatc
     3841 ccaatggcac ccgggtccga catggctcag gcccccccag ctctgggggg ggcctgtacc
     3901 gggacatggg ggcccagggg ggaaggccct ccctcatcgc caggatccct gtggccagaa
     3961 tcctggggga cccggaggaa gagtcctgga gcccctccct gactaacctg gagaaggtgg
     4021 tggtcacgga cgtgacctca aactttttga ccgtcaccat taaggaaagt aacacggacc
     4081 aaggcttttt taaagagaaa agataataag aattccatac cggttaccgg gtaggggagg
     4141 cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc acttggcgct
     4201 acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc caaccggctc
     4261 cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg aagttccccc
     4321 ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt ctcactagtc
     4381 tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc
     4441 aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt gggtccgggg
     4501 gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg
     4561 cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg
     4621 gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg tgcgcctcgc
     4681 cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg ccgactaccc
     4741 cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg agctgcaaga
     4801 actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc
     4861 cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat
     4921 cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg
     4981 cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc
     5041 gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc
     5101 cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta
     5161 cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg
     5221 gtgcatgacc cgcaagcccg gtgcctgaaa ttcgatatca agcttatcga taatcaacct
     5281 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     5341 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     5401 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     5461 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     5521 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     5581 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     5641 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     5701 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     5761 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     5821 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
     5881 gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa tgctgattgt
     5941 gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc tcaggtacct
     6001 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     6061 ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct gtggatctac
     6121 cacacacaag gctacttccc tgattggcag aactacacac cagggccagg gatcagatat
     6181 ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa ggtagaagaa
     6241 gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg gatggatgac
     6301 ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca tcacatggcc
     6361 cgagagctgc atccggactg tactgggtct ctctggttag accagatctg agcctgggag
     6421 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     6481 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     6541 tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag cctcgactgt
     6601 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
     6661 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
     6721 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
     6781 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
     6841 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
     6901 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6961 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7021 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7081 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7141 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7201 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7261 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
     7321 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     7381 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     7441 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     7501 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7561 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7621 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag
     7681 cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga
     7741 ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7801 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7861 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7921 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7981 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     8041 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     8101 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     8161 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     8221 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     8281 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8341 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     8401 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     8461 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     8521 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     8581 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     8641 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     8701 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8761 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8821 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8881 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8941 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     9001 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     9061 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     9121 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     9181 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     9241 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     9301 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     9361 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     9421 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     9481 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     9541 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     9601 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     9661 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     9721 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     9781 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     9841 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     9901 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     9961 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
    10021 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
    10081 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
    10141 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
    10201 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
    10261 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
    10321 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
    10381 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
    10441 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
    10501 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
    10561 cccgaaaagt gccacctgac gtcgacggat cgggagatct cccgatcccc tatggtgcac
    10621 tctcagtaca atctgctctg atgccgcata gttaagccag tatctgctcc ctgcttgtgt
    10681 gttggaggtc gctgagtagt gcgcgagcaa aatttaagct acaacaaggc aaggcttgac
    10741 cgacaattgc atgaagaatc tgcttagggt
//
