LOCUS       sequence-233981-        3756 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  protein expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3756)
  TITLE     Petr Svoboda lab unpublished
REFERENCE   2  (bases 1 to 3756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pSV40_HA-mPACT
FEATURES             Location/Qualifiers
     source          1..3756
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          194..326
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     309..328
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     promoter        371..389
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             407..433
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     polyA_signal    1416..1537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1427..1446)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1481..1500
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        1670..1774
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1775..1843,1844..2635)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1993..2012)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2806..3394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3295..3314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(3523..3756,1..124)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        3523..3714
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(3546..3566)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      join(3731..3756,1..110)
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
       61 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
      121 caaaaagctt gattcttctg acacaacagt ctcgaactta agctgcagaa gttggtcgtg
      181 aggcactggg caggtaagta tcaaggttac aagacaggtt taaggagacc aatagaaact
      241 gggcttgtcg agacagagaa gactcttgcg tttctgatag gcacctattg gtcttactga
      301 catccacttt gcctttctct ccacaggtgt ccactcccag ttcaattaca gctcttaagg
      361 ctagagtact taatacgact cactataggc tagcgctacc ggtatgtacc catacgacgt
      421 cccagactac gctacgcgta cggaattcgt cgactcccat agcaggcatc gtgccgaggc
      481 cccgccgctg cagcgcgagg acagcgggac cttcagtttg ggcaagatga taacagctaa
      541 gcctgggaaa acaccgattc aggtattgca cgagtacggc atgaagacca agaacattcc
      601 agtttatgaa tgtgaaagat ctgatgtgca agtacacgtg cccactttca ccttcagagt
      661 aaccgttggt gacataactt gcacaggtga aggtacaagt aagaagctgg cgaagcacag
      721 agctgcggag gccgccataa acattttgaa agccaatgca agtatttgct ttgcagttcc
      781 tgacccctta atgcctgatc catccaaaca gccgaagaac cagctgaatc caattggctc
      841 attacaggaa ttagcaattc accatggctg gcgacttcct gaatataccc tttcccagga
      901 aggaggaccg gctcataaga gggaatacac cacgatctgc aggcttgaat cattcatgga
      961 aactggaaag ggggcatcaa aaaaacaagc caagagaaat gctgctgaga aatttctcgc
     1021 caagtttagt aatatttctc cagagaacca catttctcta acgaacgtgg ttggacattc
     1081 cctaggatgc acttggcact ccttgaggaa ttcccctggt gagaagatca acctcctgaa
     1141 aaggagcctc ctcagtctcc cgaacacaga ctacatccag ctgcttagtg agattgccag
     1201 cgagcaaggc tttaacataa cgtatttgga tatagaggag ctgagtgcca acggacagta
     1261 tcagtgtctc gcagagctgt ccaccagtcc tatcaccgtg tgtcacggct caggcatctc
     1321 ctgtggcaat gcacagagtg acgctgctca caatgctctg cagtatttaa agataatagc
     1381 agaaagaaag tagcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     1441 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     1501 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     1561 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     1621 atgtggtaaa atcgataagg atccaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     1681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     1741 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     1801 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     1861 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     1921 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     1981 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
     2041 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     2101 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     2161 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     2221 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     2281 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
     2341 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     2401 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     2461 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     2521 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     2581 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     2641 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     2701 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     2761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     2821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     2881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     2941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     3001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     3061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     3121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     3181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     3241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     3301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     3361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     3421 gttcctggcc ttttgctggc cttttgctca catggctcga cagatctgcg cagcaccatg
     3481 gcctgaaata acctctgaaa gaggaacttg gttaggtacc ttctgaggcg gaaagaacca
     3541 gctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
     3601 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
     3661 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
     3721 aactccgccc atcccgcccc taactccgcc cagttc
//
