LOCUS       sequence-234034-        6876 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_195190-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6876
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2034..2051
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2103..2158
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2157..2176)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4394..4412)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4480..4584
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4585..4653,4654..5445)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4803..4822)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5616..6204
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6105..6124
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6358..6375
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6492..6513
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6528..6533,6534..6551,6552..6558)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6566..6582
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6571..6593
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6590..6606
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6590..6606
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccgaacctg
     1201 agcctgaacc agagcccgtg cccgtgtccc ggaacagcaa ggacgtgtcc atccagaccg
     1261 acgtggacct ggacccccgg ttcctgctgc tggacctgaa gagagagatc ggccggctga
     1321 aggacacctt caacgccctg gtggccaaga tcgacaccat cccccccagc agcattgcca
     1381 caggcgcgcc gtcgacctcc atcacggcct ataagagtga gggggagtca gcggagttct
     1441 ccttcccact caaccttgga gaggaaagcc tgcagggaga gttgagatgg aaggcagaga
     1501 aggctccttc ttcccagtcc tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc
     1561 agaagtctac tagcaacccc aagttccagc tgtccgaaac gctcccactc acccttcaga
     1621 taccccaggt ctcccttcag tttgctggtt ctggcaacct gaccctgact ctggacagag
     1681 ggatactgta tcaggaagtg aacctggtgg tgatgaaagt gactcagccc gacagcaaca
     1741 ctttgacctg tgaggtgatg ggacccacct cacccaagat gagactgatc ttgaagcagg
     1801 agaatcagga ggccagggtc tccaggcagg agaaagtgat tcaagtgcag gcccctgaag
     1861 caggggtgtg gcaatgtcta ctgagtgaag gtgaagaggt caagatggac tccaagatcc
     1921 aggttttatc caaagggttg aattccggca gcctgcacca catcctggac gcccagaaga
     1981 tgctgtggaa ccacagggac aggaacctgc ctcctctggc ccctctgggc ccccaccacc
     2041 atcaccacca ttgagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     2101 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2161 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     2221 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     2281 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     2341 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     2401 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     2461 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     2521 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     2581 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     2641 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     2701 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     2761 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     2821 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     2881 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     2941 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     3001 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     3061 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     3121 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     3181 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     3241 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     3301 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     3361 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     3421 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     3481 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     3541 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     3601 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     3661 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     3721 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     3781 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     3841 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     3901 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     3961 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4021 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     4081 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     4141 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4201 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     4261 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4321 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     4381 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     4441 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4501 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4561 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4621 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     4681 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     4741 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     4801 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     4861 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     4921 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     4981 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5041 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5101 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5161 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5221 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5281 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5341 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5401 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5461 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5521 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5581 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     5641 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     5701 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     5761 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     5821 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     5881 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     5941 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6001 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6061 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6121 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6181 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6241 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6301 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6361 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6421 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6481 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6541 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6601 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     6661 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     6721 ggaagatcta tacattgaat caatattggc aattagccat attagtcatt ggttatatag
     6781 cataaatcaa tattggctat tggccattgc atacgttgta tctatatcat aatatgtaca
     6841 tttatattgg ctcatgtcca atatgaccgc catgtt
//
