LOCUS       sequence-234074-        7074 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7074)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7074)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_034420-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7074
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     581..605
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1086..1105
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1128..1137
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2232..2249
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2301..2356
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2355..2374)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4592..4610)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4678..4782
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4783..4851,4852..5643)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5001..5020)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5814..6402
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6303..6322
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6556..6573
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6690..6711
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6726..6731,6732..6749,6750..6756)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6764..6780
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6769..6791
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6788..6804
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6788..6804
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt ccgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttacggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta caccaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc
      601 agatcctcac tctcttccgc atcgctgtct gcgagggcca gctgttgggc tcgcggttga
      661 ggacaaactc ttcgcggtct ttccagtact cttggatcgg aaacccgtcg gcctccgaac
      721 ggtactccgc caccgaggga cctgagcgag tccgcatcga ccggatcgga aaacctctcg
      781 agaaaggcgt ctaaccagtc acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc
      841 gggtggcggt cggggttgtt tctggcggag gtgctgctga tgatgtaatt aaagtaggcg
      901 gtcttgagac ggcggatggt cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg
      961 tgagtactcc ctctcaaaag cgggcattac ttctgcgcta agattgtcag tttccaaaaa
     1021 cgaggaggat ttgatattca cctggcccga tctggccata cacttgagtg acaatgacat
     1081 ccactttgcc tttctctcca caggtgtcca ctcccaggtc caagtttgcc accatggagt
     1141 ttcagaccca ggtactcatg tccctgctgc tctgcatgtc tggtgcggcc gccaatgtgg
     1201 gaatgcctgg cgcccctcgg aagctgcagc tgaacaacgc caccatcgag aagttcggcc
     1261 acgagctggc caaggtggcc cacaagttca acagcgagta cggcaacagc agcctgcacg
     1321 tgatcaccaa gctcgtgaac gtgaccagcc aggtggtgca gggcatcttc tacacctttt
     1381 tcgtgaagtt cagccccacc agctgcacca ccgccaacaa caacatccac gtgctggaca
     1441 cctacttccc caaccgggac agctgcgacc tgaccgacgg cgagagcaag atctgcaaag
     1501 tgaccctgtg ggaccggccc tggctgcaga gcgacttcag caagatcatc cggatcgtgc
     1561 ggtgcagcaa cgacacctct ggcgcgccgt cgacctccat cacggcctat aagagtgagg
     1621 gggagtcagc ggagttctcc ttcccactca accttggaga ggaaagcctg cagggagagt
     1681 tgagatggaa ggcagagaag gctccttctt cccagtcctg gatcaccttc tccctaaaga
     1741 accaaaaggt gtctgtgcag aagtctacta gcaaccccaa gttccagctg tccgaaacgc
     1801 tcccactcac ccttcagata ccccaggtct cccttcagtt tgctggttct ggcaacctga
     1861 ccctgactct ggacagaggg atactgtatc aggaagtgaa cctggtggtg atgaaagtga
     1921 ctcagcccga cagcaacact ttgacctgtg aggtgatggg acccacctca cccaagatga
     1981 gactgatctt gaagcaggag aatcaggagg ccagggtctc caggcaggag aaagtgattc
     2041 aagtgcaggc ccctgaagca ggggtgtggc aatgtctact gagtgaaggt gaagaggtca
     2101 agatggactc caagatccag gttttatcca aagggttgaa ttccggcagc ctgcaccaca
     2161 tcctggacgc ccagaagatg ctgtggaacc acagggacag gaacctgcct cctctggccc
     2221 ctctgggccc ccaccaccat caccaccatt gagtctagac acgtgattaa ttaaggatcc
     2281 cccgacctcg acctctggct aataaaggaa atttattttc attgcaatag tgtgttggaa
     2341 ttttttgtgt ctctcactcg gaaggacata tgggagggca aatcatttgg tcgagatccc
     2401 tcggagatct ctagctagag gatcgatccc cgccccggac gaactaaacc tgactacgac
     2461 atctctgccc cttcttcgcg gggcagtgca tgtaatccct tcagttggtt ggtacaactt
     2521 gccaactggg ccctgttcca catgtgacac ggggggggac caaacacaaa ggggttctct
     2581 gactgtagtt gacatcctta taaatggatg tgcacatttg ccaacactga gtggctttca
     2641 tcctggagca gactttgcag tctgtggact gcaacacaac attgccttta tgtgtaactc
     2701 ttggctgaag ctcttacacc aatgctgggg gacatgtacc tcccaggggc ccaggaagac
     2761 tacgggaggc tacaccaacg tcaatcagag gggcctgtgt agctaccgat aagcggaccc
     2821 tcaagagggc attagcaata gtgtttataa ggcccccttg ttaaccctaa acgggtagca
     2881 tatgcttccc gggtagtagt atatactatc cagactaacc ctaattcaat agcatatgtt
     2941 acccaacggg aagcatatgc tatcgaatta gggttagtaa aagggtccta aggaacagcg
     3001 atatctccca ccccatgagc tgtcacggtt ttatttacat ggggtcagga ttccacgagg
     3061 gtagtgaacc attttagtca caagggcagt ggctgaagat caaggagcgg gcagtgaact
     3121 ctcctgaatc ttcgcctgct tcttcattct ccttcgttta gctaatagaa taactgctga
     3181 gttgtgaaca gtaaggtgta tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca
     3241 gaataaaatt tggacggggg gttcagtggt ggcattgtgc tatgacacca atataaccct
     3301 cacaaacccc ttgggcaata aatactagtg taggaatgaa acattctgaa tatctttaac
     3361 aatagaaatc catggggtgg ggacaagccg taaagactgg atgtccatct cacacgaatt
     3421 tatggctatg ggcaacacat aatcctagtg caatatgata ctggggttat taagatgtgt
     3481 cccaggcagg gaccaagaca ggtgaaccat gttgttacac tctatttgta acaaggggaa
     3541 agagagtgga cgccgacagc agcggactcc actggttgtc tctaacaccc ccgaaaatta
     3601 aacggggctc cacgccaatg gggcccataa acaaagacaa gtggccactc ttttttttga
     3661 aattgtggag tgggggcacg cgtcagcccc cacacgccgc cctgcggttt tggactgtaa
     3721 aataagggtg taataacttg gctgattgta accccgctaa ccactgcggt caaaccactt
     3781 gcccacaaaa ccactaatgg caccccgggg aatacctgca taagtaggtg ggcgggccaa
     3841 gataggggcg cgattgctgc gatctggagg acaaattaca cacacttgcg cctgagcgcc
     3901 aagcacaggg ttgttggtcc tcatattcac gaggtcgctg agagcacggt gggctaatgt
     3961 tgccatgggt agcatatact acccaaatat ctggatagca tatgctatcc taatctatat
     4021 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4081 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     4141 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     4201 ccgggtagca tatgctatcc taatagagat tagggtagta tatgctatcc taatttatat
     4261 ctgggtagca tatactaccc aaatatctgg atagcatatg ctatcctaat ctatatctgg
     4321 gtagcatatg ctatcctaat ctatatctgg gtagcatagg ctatcctaat ctatatctgg
     4381 gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat ttatatctgg
     4441 gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat ctatatctgg
     4501 gtagtatatg ctatcctaat ctgtatccgg gtagcatatg ctatcctcat gataagctgt
     4561 caaacatgag aattttcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4621 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4681 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4741 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4801 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4861 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4921 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4981 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa
     5041 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     5101 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     5161 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     5221 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     5281 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca
     5341 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     5401 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     5461 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     5521 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     5581 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5641 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5701 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5761 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5821 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5881 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5941 gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac
     6001 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     6061 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     6121 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     6181 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     6241 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     6301 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     6361 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     6421 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     6481 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     6541 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     6601 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     6661 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     6721 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     6781 tttcacacag gaaacagcta tgaccatgat tacgccaagc tctagctaga ggtcgaccaa
     6841 ttctcatgtt tgacagctta tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg
     6901 cgcagaactg gtaggtatgg aagatctata cattgaatca atattggcaa ttagccatat
     6961 tagtcattgg ttatatagca taaatcaata ttggctattg gccattgcat acgttgtatc
     7021 tatatcataa tatgtacatt tatattggct catgtccaat atgaccgcca tgtt
//
