LOCUS       sequence-234177-        1596 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express His-tagged PKM2 (R399E) protein in bacteria..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Chen TJ, Wang HJ, Liu JS, Cheng HH, Hsu SC, Wu MC, Lu CH, Wu YF, Wu 
            JW, Liu YY, Kung HJ, Wang WC
  TITLE     Mutations in the PKM2 exon-10 region are associated with reduced 
            allostery and increased nuclear translocation.
  JOURNAL   Commun Biol. 2019 Mar 15;2:105. doi: 10.1038/s42003-019-0343-4. 
            eCollection 2019.
  PUBMED    30911680
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2019-03-15"; volume: "2:105. doi"; pages: " 
            10.1038/s42003-019-0343-4. eCollection 2019"
COMMENT     Sequence Label: pET28a-PKM2-R399E
FEATURES             Location/Qualifiers
     source          1..1596
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 atgtcgaagc cccatagtga agccgggact gccttcattc agacccagca gctgcacgca
       61 gccatggctg acacattcct ggagcacatg tgccgcctgg acattgattc accacccatc
      121 acagcccgga acactggcat catctgtacc attggcccag cttcccgatc agtggagacg
      181 ttgaaggaga tgattaagtc tggaatgaat gtggctcgtc tgaacttctc tcatggaact
      241 catgagtacc atgcggagac catcaagaat gtgcgcacag ccacggaaag ctttgcttct
      301 gaccccatcc tctaccggcc cgttgctgtg gctctagaca ctaaaggacc tgagatccga
      361 actgggctca tcaagggcag cggcactgca gaggtggagc tgaagaaggg agccactctc
      421 aaaatcacgc tggataacgc ctacatggaa aagtgtgacg agaacatcct gtggctggac
      481 tacaagaaca tctgcaaggt ggtggaagtg ggcagcaaga tctacgtgga tgatgggctt
      541 atttctctcc aggtgaagca gaaaggtgcc gacttcctgg tgacggaggt ggaaaatggt
      601 ggctccttgg gcagcaagaa gggtgtgaac cttcctgggg ctgctgtgga cttgcctgct
      661 gtgtcggaga aggacatcca ggatctgaag tttggggtcg agcaggatgt tgatatggtg
      721 tttgcgtcat tcatccgcaa ggcatctgat gtccatgaag ttaggaaggt cctgggagag
      781 aagggaaaga acatcaagat tatcagcaaa atcgagaatc atgagggggt tcggaggttt
      841 gatgaaatcc tggaggccag tgatgggatc atggtggctc gtggtgatct aggcattgag
      901 attcctgcag agaaggtctt ccttgctcag aagatgatga ttggacggtg caaccgagct
      961 gggaagcctg tcatctgtgc tactcagatg ctggagagca tgatcaagaa gccccgcccc
     1021 actcgggctg aaggcagtga tgtggccaat gcagtcctgg atggagccga ctgcatcatg
     1081 ctgtctggag aaacagccaa aggggactat cctctggagg ctgtgcgcat gcagcacctg
     1141 attgcccgtg aggcagaggc tgccatctac cacttgcaat tatttgagga actcgagcgc
     1201 ctggcgccca ttaccagcga ccccacagaa gccaccgccg tgggtgccgt ggaggcctcc
     1261 ttcaagtgct gcagtggggc cataatcgtc ctcaccaagt ctggcaggtc tgctcaccag
     1321 gtggccagat accgcccacg tgcccccatc attgctgtga cccggaatcc ccagacagct
     1381 cgtcaggccc acctgtaccg tggcatcttc cctgtgctgt gcaaggaccc agtccaggag
     1441 gcctgggctg aggacgtgga cctccgggtg aactttgcca tgaatgttgg caaggcccga
     1501 ggcttcttca agaagggaga tgtggtcatt gtgctgaccg gatggcgccc tggctccggc
     1561 ttcaccaaca ccatgcgtgt tgttcctgtg ccgtga
//
