LOCUS       sequence-234399-        4843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cyanobacteria cloning vector. Chromosomal integration into 
            Synechococcus elongatus neutral site 1 (NS1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Taton A, Unglaub F, Wright NE, Zeng WY, Paz-Yepez J, Brahamsha B, 
            Palenik B, Peterson TC, Haerizadeh F, Golden SS, Golden JW
  TITLE     Broad-host-range vector system for synthetic biology and 
            biotechnology in cyanobacteria.
  JOURNAL   Nucleic Acids Res. 2014 Jul 29. pii: gku673.
  PUBMED    25074377
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2014 Jul 29. pii: gku673."
COMMENT     Sequence Label: pAM4951 (pCV0063)
FEATURES             Location/Qualifiers
     source          1..4843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      823..1411
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1312..1331
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1565..1582
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    1597..1737
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(1663..1682)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(2766..3554)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     CDS             4408..4713
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     4592..4611
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     terminator      4781..4808
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tctagattct ggagcaggaa gatgtcgcgg gcattagcac cagcggtctg ccaagcctcc
       61 gccagccgtt gggtcccttc cgcttgagct tttccatctt cgacgatacg ggcggcggcc
      121 ccccgcgctt ccgcgatcgc ccgtttacaa gctgcctcag ctggggcgat cacatcggct
      181 tgaagttgct gctgcacctg tttgatccgc tcctgctgca cagggagttc tgcttggcta
      241 cgagcgactt cggtagcaat gtccgcttca gcttcggcca ccaccgcttc gcgccgcgtc
      301 aacgcatcct gaatccggcg ctcggcctcg gcttgggcga tcgctacatc gcgatcgatc
      361 cgacgcaggg ccgtgatctt gtcattttcg gccgtttgga tcgcagaggc agcctgggca
      421 tcggcttcag caattcgggc atctcgctgc agatcagccc gctgcttgcg tccactagcc
      481 gagagataac cgacctcatc ggaaatgttc tggacttgca gcgtatcgag gactagaccc
      541 agctgctcaa ggtcatcctc cgcctcttcc agcagacttt tggcaaaggc aattttgtcc
      601 tcgttgatct gctccggcgt gaggctggct aaaacaccac gcaagttgcc ttcgagggtc
      661 tccttggcaa tttgctcgat ttccttacgg tttttgccaa gcagccgctc gatcgcgttg
      721 tggatggtcg gttcttcccc agcaatcttg atattggcaa cgccttcaac agtcagggga
      781 atgccgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
      841 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
      901 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
      961 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     1021 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     1081 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     1141 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata
     1201 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta
     1261 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc
     1321 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg
     1381 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
     1441 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
     1501 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
     1561 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac
     1621 gcatctgtgc ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc
     1681 cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc
     1741 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc gccatccgct
     1801 tacagacaag ctccgcggcg gagcgctgct ttcttggcaa gcggtcgcca gccccaacgc
     1861 cagggctgcc agcccgaaac agcggggcaa ggcagcttgg aagggcgatc gcagcacggg
     1921 catggcaatg tctctctgaa ggaatgcaga ccttattcgt acagccaggg ttgaatcgtg
     1981 ggggtccaat cacttagctc tgctgggcta aaccagagag caatttcctg ttgtgctgtt
     2041 tcgattgcat ccgagccatg gatgatgttg cggccaatat tgacaccaaa atcaccacgg
     2101 atggtgcccg gttctgccgt cagcggattg gtagcgccga tcaacttgcg agcagccgcc
     2161 acaacgcctt cgccttccaa gacgatcgcc acgatcggcc cagaggtgat gaactcgacg
     2221 aggccattga agaaggggcg ctcgcggtgg acagcatagt gctgttcggc cagctcgcga
     2281 ctgggcttca gctgctttag gcccaccagt ttgaagcctt tttgctcaaa gcggccgatg
     2341 atcgtaccga ccaaaccccg ctgaacgcca tcgggcttga tggcaataaa tgtgcgttcc
     2401 acagacatct agatagtcct caagacgagg caagcattga gcttgccttc ctatggttcg
     2461 ggatcactgg gattcttgac aagcgatcgc ggtcacatcg ctatctctta ggacttcgca
     2521 gcgggcgagt cggattgacc cggtagggat ttcgccagat caatgcccgt ggtttgtttc
     2581 agcttctcca gcaagctagc gatttgggta gcgctgcctt ccccttcgcc aatcacagtg
     2641 atcgactcca cgtcgatatc tggcacggtg cctgaaagcg tgacgagcag ctagcatcgc
     2701 gcgcgcgcgc gcggatgcta gcggcgcgcc aaaaaaaagc ccgctcatta ggcgggctgg
     2761 ccggccttgc cgactacctt ggtgatctcg cctttcacgt agtggacaaa ttcttccaac
     2821 tgatctgcgc gcgaggccaa gcgatcttct tcttgtccaa gataagcctg tctagcttca
     2881 agtatgacgg gctgatactg ggccggcagg cgctccattg cccagtcggc agcgacatcc
     2941 ttcggcgcga ttttgccggt tactgcgctg taccaaatgc gggacaacgt aagcactaca
     3001 tttcgctcat cgccagccca gtcgggcggc gagttccata gcgttaaggt ttcatttagc
     3061 gcctcaaata gatcctgttc aggaaccgga tcaaagagtt cctccgccgc tggacctacc
     3121 aaggcaacgc tatgttctct tgcttttgtc agcaagatag ccagatcaat gtcgatcgtg
     3181 gctggctcga agatacctgc aagaatgtca ttgcgctgcc attctccaaa ttgcagttcg
     3241 cgcttagctg gataacgcca cggaatgatg tcgtcgtgca caacaatggt gacttctaca
     3301 gcgcggagaa tctcgctctc tccaggggaa gccgaagttt ccaaaaggtc gttgatcaaa
     3361 gctcgccgcg ttgtttcatc aagccttacg gtcaccgtaa ccagcaaatc aatatcactg
     3421 tgtggcttca ggccgccatc cactgcggag ccgtacaaat gtacggccag caacgtcggt
     3481 tcgagatggc gctcgatgac gccaactacc tctgatagtt gagtcgatac ttcggcgatc
     3541 accgcttccc tcatgatgtt taactttgtt ttagggcgac tgccctgctg cgtaacatcg
     3601 ttgctgctcc ataacatcaa acatcgaccc acggcgtaac gcgcttgctg cttggatgcc
     3661 cgaggcatag actgtacccc aaaaaaacag tcataacaag ccatgaaaac cgccactgcg
     3721 ccgttaccac cgctgcgttc ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac
     3781 ttgcattaca gcttacgaac cgaacaggct tatgtccact gggttcgtgc cttcatccgt
     3841 ttccacggtg tgcgtcaccc ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc
     3901 tggctggcga acgagcgcaa ggtttcggtc tccacgcatt cgaaaccggt atcccgccgc
     3961 cgccgccgga taccggttaa tagtacctgt gaagtgaaaa atggcgcaca ttgtgcgaca
     4021 tttttttctc gagcaggtag tcggccaata acccagggat ttaaatacgc gtggatccgg
     4081 cttactaaaa gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat
     4141 atatactgat atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt
     4201 acagtgacag ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct
     4261 ccggtctggt aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga
     4321 aagcggaaaa tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt
     4381 ttgctgacga gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag
     4441 agccgttatc gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg
     4501 atggtgatcc ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac
     4561 ccggtggtgc atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg
     4621 ccggtctccg ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa
     4681 aacgccatta acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag
     4741 tctgcaggtc gacatttaaa tgaaggagct ccccggcagg agaaggccat cctgacggat
     4801 ggcctttttg aagctttcta gaatcggggg cccccggggg gat
//
