LOCUS       sequence-234404-        8088 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_052660-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..8088
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2866..2883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2935..2990
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2989..3008)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5226..5244)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5312..5416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5417..5485,5486..6277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5635..5654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6448..7036
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6937..6956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7190..7207
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7324..7345
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7360..7365,7366..7383,7384..7390)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7398..7414
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7403..7425
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7422..7438
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7422..7438
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7709..8088
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccatcaact gcctgcagcg gaagtacgac
      841 aagaacaccg tggccagcat ccccggcgac atcatgctgg gcggcctgtt ccctgtgcac
      901 accagcggcg tgaccacatg cgagagcatc aaccccgagc ggggcatcca gcgggtggaa
      961 gccatgctgt tcaccctgga cgagatcaac aacaacagca agctgctgcc cggcctgacc
     1021 ctgggcacca ccatcagaga tacctgcagc gacaccaacc acgccctgga acaggccctg
     1081 aagttcgtgc aggccagcac cggcagcagc agccagctga acaagatgaa cgagcacagc
     1141 gagaacctga acaccagagg cgtcgtgggc agcagctaca gcagcgtgac catcctggtg
     1201 gccaacctgt tccggctgtt cagcctgccc cagatcagct acgccagcac caccgccatc
     1261 ctgagcgaca agcgggcctt ccctctgttc gcccggaccg tgccttccga cgtggtgcag
     1321 gcccaggcca tggccacact ggtgtccgcc cacaactgga cctacgtgtc caccgtgcgc
     1381 tctgccggcg actacggcga cagcggcatg gacgcctttt ggaaagaggc cgacaagctg
     1441 ggcgtgtgta tcgccgccag agaagtgatc cggggcaaca ccggccctga ggacctggac
     1501 aacatcgtga acgtgctgag caaggacttc gccaaggccc gggtggtggt gctgtttacc
     1561 ggcatggacc acacaaagct gctgctggac gccgtgcgga gagccggcct ggtcaaccac
     1621 ttcgtgtgga tcgccagcga cggctggggc agagaaaacg tgcccgtgtc caacaactcc
     1681 agagaggcca acggcgccct gaccatcgag atccaggccg agcccatcaa ggccttcacc
     1741 gactactacc tgagcctggg cagagagaac caccggaacc cctggttcag agagtactgg
     1801 caggacctgc tgcagtgcag agagagcagc accaagcgga gagagcggaa gtccaccagc
     1861 agccggcggt acttcatgag caagctgagc aagaacgaga caaacctgga aaacagccac
     1921 gagatcagca ccgagagcag ctccctgccc agcagctgca gcgactcccc caacctgaag
     1981 ctgcgggaca tcctgggccc cgacttcaag caggaagcca agatccagtt cgtgtacgac
     2041 gccgtgtacg ccttcgccag cggcctgcac atcctgcaga aaaagctgtg ccccggcaac
     2101 ccccaccacc ccaagtggaa caagcggagc tgcctggaaa agatgctgag ctaccccggc
     2161 accgccttct accagctgat catcaacacc agcttcaccg gcgagttcat cagaggcgcg
     2221 ccgtcgacct ccatcacggc ctataagagt gagggggagt cagcggagtt ctccttccca
     2281 ctcaaccttg gagaggaaag cctgcaggga gagttgagat ggaaggcaga gaaggctcct
     2341 tcttcccagt cctggatcac cttctcccta aagaaccaaa aggtgtctgt gcagaagtct
     2401 actagcaacc ccaagttcca gctgtccgaa acgctcccac tcacccttca gataccccag
     2461 gtctcccttc agtttgctgg ttctggcaac ctgaccctga ctctggacag agggatactg
     2521 tatcaggaag tgaacctggt ggtgatgaaa gtgactcagc ccgacagcaa cactttgacc
     2581 tgtgaggtga tgggacccac ctcacccaag atgagactga tcttgaagca ggagaatcag
     2641 gaggccaggg tctccaggca ggagaaagtg attcaagtgc aggcccctga agcaggggtg
     2701 tggcaatgtc tactgagtga aggtgaagag gtcaagatgg actccaagat ccaggtttta
     2761 tccaaagggt tgaattccgg cagcctgcac cacatcctgg acgcccagaa gatgctgtgg
     2821 aaccacaggg acaggaacct gcctcctctg gcccctctgg gcccccacca ccatcaccac
     2881 cattgagtct agacacgtga ttaattaagg atcccccgac ctcgacctct ggctaataaa
     2941 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
     3001 catatgggag ggcaaatcat ttggtcgaga tccctcggag atctctagct agaggatcga
     3061 tccccgcccc ggacgaacta aacctgacta cgacatctct gccccttctt cgcggggcag
     3121 tgcatgtaat cccttcagtt ggttggtaca acttgccaac tgggccctgt tccacatgtg
     3181 acacgggggg ggaccaaaca caaaggggtt ctctgactgt agttgacatc cttataaatg
     3241 gatgtgcaca tttgccaaca ctgagtggct ttcatcctgg agcagacttt gcagtctgtg
     3301 gactgcaaca caacattgcc tttatgtgta actcttggct gaagctctta caccaatgct
     3361 gggggacatg tacctcccag gggcccagga agactacggg aggctacacc aacgtcaatc
     3421 agaggggcct gtgtagctac cgataagcgg accctcaaga gggcattagc aatagtgttt
     3481 ataaggcccc cttgttaacc ctaaacgggt agcatatgct tcccgggtag tagtatatac
     3541 tatccagact aaccctaatt caatagcata tgttacccaa cgggaagcat atgctatcga
     3601 attagggtta gtaaaagggt cctaaggaac agcgatatct cccaccccat gagctgtcac
     3661 ggttttattt acatggggtc aggattccac gagggtagtg aaccatttta gtcacaaggg
     3721 cagtggctga agatcaagga gcgggcagtg aactctcctg aatcttcgcc tgcttcttca
     3781 ttctccttcg tttagctaat agaataactg ctgagttgtg aacagtaagg tgtatgtgag
     3841 gtgctcgaaa acaaggtttc aggtgacgcc cccagaataa aatttggacg gggggttcag
     3901 tggtggcatt gtgctatgac accaatataa ccctcacaaa ccccttgggc aataaatact
     3961 agtgtaggaa tgaaacattc tgaatatctt taacaataga aatccatggg gtggggacaa
     4021 gccgtaaaga ctggatgtcc atctcacacg aatttatggc tatgggcaac acataatcct
     4081 agtgcaatat gatactgggg ttattaagat gtgtcccagg cagggaccaa gacaggtgaa
     4141 ccatgttgtt acactctatt tgtaacaagg ggaaagagag tggacgccga cagcagcgga
     4201 ctccactggt tgtctctaac acccccgaaa attaaacggg gctccacgcc aatggggccc
     4261 ataaacaaag acaagtggcc actctttttt ttgaaattgt ggagtggggg cacgcgtcag
     4321 cccccacacg ccgccctgcg gttttggact gtaaaataag ggtgtaataa cttggctgat
     4381 tgtaaccccg ctaaccactg cggtcaaacc acttgcccac aaaaccacta atggcacccc
     4441 ggggaatacc tgcataagta ggtgggcggg ccaagatagg ggcgcgattg ctgcgatctg
     4501 gaggacaaat tacacacact tgcgcctgag cgccaagcac agggttgttg gtcctcatat
     4561 tcacgaggtc gctgagagca cggtgggcta atgttgccat gggtagcata tactacccaa
     4621 atatctggat agcatatgct atcctaatct atatctgggt agcataggct atcctaatct
     4681 atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct atcctaattt
     4741 atatctgggt agcataggct atcctaatct atatctgggt agcatatgct atcctaatct
     4801 atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct atcctaatag
     4861 agattagggt agtatatgct atcctaattt atatctgggt agcatatact acccaaatat
     4921 ctggatagca tatgctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     4981 ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc taatctatat
     5041 ctgggtagta tatgctatcc taatttatat ctgggtagca taggctatcc taatctatat
     5101 ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc taatctgtat
     5161 ccgggtagca tatgctatcc tcatgataag ctgtcaaaca tgagaatttt cttgaagacg
     5221 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
     5281 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5341 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5401 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5461 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5521 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5581 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5641 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5701 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5761 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5821 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5881 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5941 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     6001 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6061 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6121 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6181 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6241 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6301 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6361 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6421 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6481 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6541 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6601 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6661 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6721 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6781 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6841 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6901 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6961 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     7021 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7081 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7141 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7201 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7261 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7321 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7381 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7441 tgattacgcc aagctctagc tagaggtcga ccaattctca tgtttgacag cttatcatcg
     7501 cagatccggg caacgttgtt gccattgctg caggcgcaga actggtaggt atggaagatc
     7561 tatacattga atcaatattg gcaattagcc atattagtca ttggttatat agcataaatc
     7621 aatattggct attggccatt gcatacgttg tatctatatc ataatatgta catttatatt
     7681 ggctcatgtc caatatgacc gccatgttga cattgattat tgactagtta ttaatagtaa
     7741 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     7801 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     7861 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     7921 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
     7981 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
     8041 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatg
//
