LOCUS       sequence-234482-        7656 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_103610-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2434..2451
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2503..2558
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2557..2576)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4794..4812)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4880..4984
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4985..5053,5054..5845)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5203..5222)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6016..6604
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6505..6524
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6758..6775
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6892..6913
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6928..6933,6934..6951,6952..6958)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6966..6982
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6971..6993
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6990..7006
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6990..7006
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        7277..7656
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gcccacatca gcgtgaagaa cgagaagttc
      841 gagcccctga gcgacgacat catcagctac atcaacgagc accccaacgc cggctggcgg
      901 gctgagaagt ccaaccggtt ccacagcctg gacgacgccc ggatccagat gggcgccaga
      961 agagaggaac ccgacctgcg gagaaccaga cggcccaccg tggaccacaa cgactggaac
     1021 gtggaaatcc ccagcagctt cgacagccgg aagaaatggc cccggtgcaa gtctatcgcc
     1081 accatccggg accagagcag atgcggcagc tgctgggcct ttggcgccgt ggaagccatg
     1141 agcgacagaa gctgcatcca gagcggcggc aagcagaacg tggaactgag cgccgtggac
     1201 ctgctgagct gctgcgagtc ttgtggcctg ggctgcgagg gcggaatcct gggccctgcc
     1261 tgggactact gggtcaaaga gggcatcgtc accggcagca gcaaagagaa ccacaccggc
     1321 tgcgagccct acccattccc caagtgcgag caccacacca agggcaagta ccccccctgc
     1381 ggcagcaaga tctacaagac ccccagatgc aagcagacct gccagaagaa gtacaagaca
     1441 ccctacaccc aggacaagca ccggggcaag agcagctaca acgtgaagaa tgacgagaag
     1501 gccatccaga aagaaatcat gaagtacggc cccgtcgagg ccggctttac cgtgtacgag
     1561 gacttcctga actacaagag cggcatctac aagcacatca ccggcgagac actgggcggc
     1621 cacgccatca gaatcatcgg ctggggcgtg gaaaacaaga ccccttactg gctgatcgcc
     1681 aacagctgga acgaggactg gggcgagaac ggctacttcc ggatcgtgcg gggcagggac
     1741 gagtgcagca tcgagagcga agtgaccgcc ggcagaatca atggcgcgcc gtcgacctcc
     1801 atcacggcct ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga
     1861 gaggaaagcc tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc
     1921 tggatcacct tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc
     1981 aagttccagc tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag
     2041 tttgctggtt ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg
     2101 aacctggtgg tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg
     2161 ggacccacct cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc
     2221 tccaggcagg agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta
     2281 ctgagtgaag gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg
     2341 aattccggca gcctgcacca catcctggac gcccagaaga tgctgtggaa ccacagggac
     2401 aggaacctgc ctcctctggc ccctctgggc ccccaccacc atcaccacca ttgagtctag
     2461 acacgtgatt aattaaggat cccccgacct cgacctctgg ctaataaagg aaatttattt
     2521 tcattgcaat agtgtgttgg aattttttgt gtctctcact cggaaggaca tatgggaggg
     2581 caaatcattt ggtcgagatc cctcggagat ctctagctag aggatcgatc cccgccccgg
     2641 acgaactaaa cctgactacg acatctctgc cccttcttcg cggggcagtg catgtaatcc
     2701 cttcagttgg ttggtacaac ttgccaactg ggccctgttc cacatgtgac acgggggggg
     2761 accaaacaca aaggggttct ctgactgtag ttgacatcct tataaatgga tgtgcacatt
     2821 tgccaacact gagtggcttt catcctggag cagactttgc agtctgtgga ctgcaacaca
     2881 acattgcctt tatgtgtaac tcttggctga agctcttaca ccaatgctgg gggacatgta
     2941 cctcccaggg gcccaggaag actacgggag gctacaccaa cgtcaatcag aggggcctgt
     3001 gtagctaccg ataagcggac cctcaagagg gcattagcaa tagtgtttat aaggccccct
     3061 tgttaaccct aaacgggtag catatgcttc ccgggtagta gtatatacta tccagactaa
     3121 ccctaattca atagcatatg ttacccaacg ggaagcatat gctatcgaat tagggttagt
     3181 aaaagggtcc taaggaacag cgatatctcc caccccatga gctgtcacgg ttttatttac
     3241 atggggtcag gattccacga gggtagtgaa ccattttagt cacaagggca gtggctgaag
     3301 atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg cttcttcatt ctccttcgtt
     3361 tagctaatag aataactgct gagttgtgaa cagtaaggtg tatgtgaggt gctcgaaaac
     3421 aaggtttcag gtgacgcccc cagaataaaa tttggacggg gggttcagtg gtggcattgt
     3481 gctatgacac caatataacc ctcacaaacc ccttgggcaa taaatactag tgtaggaatg
     3541 aaacattctg aatatcttta acaatagaaa tccatggggt ggggacaagc cgtaaagact
     3601 ggatgtccat ctcacacgaa tttatggcta tgggcaacac ataatcctag tgcaatatga
     3661 tactggggtt attaagatgt gtcccaggca gggaccaaga caggtgaacc atgttgttac
     3721 actctatttg taacaagggg aaagagagtg gacgccgaca gcagcggact ccactggttg
     3781 tctctaacac ccccgaaaat taaacggggc tccacgccaa tggggcccat aaacaaagac
     3841 aagtggccac tctttttttt gaaattgtgg agtgggggca cgcgtcagcc cccacacgcc
     3901 gccctgcggt tttggactgt aaaataaggg tgtaataact tggctgattg taaccccgct
     3961 aaccactgcg gtcaaaccac ttgcccacaa aaccactaat ggcaccccgg ggaatacctg
     4021 cataagtagg tgggcgggcc aagatagggg cgcgattgct gcgatctgga ggacaaatta
     4081 cacacacttg cgcctgagcg ccaagcacag ggttgttggt cctcatattc acgaggtcgc
     4141 tgagagcacg gtgggctaat gttgccatgg gtagcatata ctacccaaat atctggatag
     4201 catatgctat cctaatctat atctgggtag cataggctat cctaatctat atctgggtag
     4261 catatgctat cctaatctat atctgggtag tatatgctat cctaatttat atctgggtag
     4321 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4381 tatatgctat cctaatctgt atccgggtag catatgctat cctaatagag attagggtag
     4441 tatatgctat cctaatttat atctgggtag catatactac ccaaatatct ggatagcata
     4501 tgctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagcata
     4561 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     4621 tgctatccta atttatatct gggtagcata ggctatccta atctatatct gggtagcata
     4681 tgctatccta atctatatct gggtagtata tgctatccta atctgtatcc gggtagcata
     4741 tgctatcctc atgataagct gtcaaacatg agaattttct tgaagacgaa agggcctcgt
     4801 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     4861 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     4921 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     4981 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     5041 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     5101 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     5161 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     5221 atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     5281 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     5341 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     5401 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     5461 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     5521 gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     5581 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     5641 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     5701 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     5761 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     5821 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     5881 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     5941 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     6001 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     6061 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     6121 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     6181 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     6241 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     6301 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     6361 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     6421 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     6481 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     6541 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     6601 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     6661 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     6721 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     6781 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag
     6841 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
     6901 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     6961 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     7021 gctctagcta gaggtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca
     7081 acgttgttgc cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat
     7141 caatattggc aattagccat attagtcatt ggttatatag cataaatcaa tattggctat
     7201 tggccattgc atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca
     7261 atatgaccgc catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     7321 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     7381 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     7441 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     7501 cacttggcag tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac
     7561 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg
     7621 cagtacatct acgtattagt catcgctatt accatg
//
