LOCUS       sequence-234501-        7662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7662)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 7662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_002150-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..7662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..302
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      325..334
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2017..2034
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    2086..2141
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2140..2159)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4377..4395)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4463..4567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4568..4636,4637..5428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4786..4805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5599..6187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6088..6107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6341..6358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6475..6496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6511..6516,6517..6534,6535..6541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6549..6565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6554..6576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6573..6589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6573..6589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6860..7239
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7240..7443
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     7394..7414
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     7440..7464
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gtcgcaaggt aggctgagca ccgtggcggg cggcagcggg tggcggtcgg ggttgtttct
       61 ggcggaggtg ctgctgatga tgtaattaaa gtaggcggtc ttgagacggc ggatggtcga
      121 ggtgaggtgt ggcaggcttg agatccagct gttggggtga gtactccctc tcaaaagcgg
      181 gcattacttc tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct
      241 ggcccgatct ggccatacac ttgagtgaca atgacatcca ctttgccttt ctctccacag
      301 gtgtccactc ccaggtccaa gtttgccacc atggagtttc agacccaggt actcatgtcc
      361 ctgctgctct gcatgtctgg tgcggccgcc aacgagatcg acgccagacg gcacaagcgg
      421 atgtaccagc ccctgagcat ggaactgatc aacttcatca actacgaggc caacaccacc
      481 tggaaggccg ctcccaccac ccggttccgg accgtgtccg acatcagacg gatgctgggc
      541 gccctgcccg accccaatgg cgagcagctg gaaaccctgt gcaccggcta catcagcgac
      601 gagctgccca agagcttcga cgcccgggtg gaatggcccc actgccccag catcagcgag
      661 atccgggatc agagcagctg cggctcctgc tgggcctttg gcgccgtgga agccatgagc
      721 gaccggatct gcatcaagag caagggcaag cacaagccct ttctgagcgc cgagaacctg
      781 gtgtcctgct gcagctcctg cggcatgggc tgcaacggcg gctttcctca cagcgcctgg
      841 ctgtactgga agaaccaggg catcgtgacc ggcgacctgt acaacaccac caacggctgc
      901 cagccctacg agttcccccc atgcgagcac cacgtgatcg gccccctgcc cagctgtgac
      961 ggcgacgtgg aaacccccag ctgcaagacc aactgccagc ccggctacaa catcccctac
     1021 gagaaggaca agtggtacgg cgagaaggtg taccggatcc acagcaaccc cgaggccatc
     1081 atgctggaac tgatgcggaa cggccccgtg gaagtggact tcgaggtgta cgccgacttc
     1141 cccaactaca agagcggcgt gtaccagcac gtgtcaggcg ccctgctggg aggacacgcc
     1201 gtcagactgc tgggctgggg cgaagagaac aacgtgccct actggctgat cgccaacagc
     1261 tggaacagcg actggggcga caagggctac ttcaagatcg tgcggggcaa gaacgagtgc
     1321 ggcatcgaga gcgacgtgaa cgccggcatc cccaagatca agaatggcgc gccgtcgacc
     1381 tccatcacgg cctataagag tgagggggag tcagcggagt tctccttccc actcaacctt
     1441 ggagaggaaa gcctgcaggg agagttgaga tggaaggcag agaaggctcc ttcttcccag
     1501 tcctggatca ccttctccct aaagaaccaa aaggtgtctg tgcagaagtc tactagcaac
     1561 cccaagttcc agctgtccga aacgctccca ctcacccttc agatacccca ggtctccctt
     1621 cagtttgctg gttctggcaa cctgaccctg actctggaca gagggatact gtatcaggaa
     1681 gtgaacctgg tggtgatgaa agtgactcag cccgacagca acactttgac ctgtgaggtg
     1741 atgggaccca cctcacccaa gatgagactg atcttgaagc aggagaatca ggaggccagg
     1801 gtctccaggc aggagaaagt gattcaagtg caggcccctg aagcaggggt gtggcaatgt
     1861 ctactgagtg aaggtgaaga ggtcaagatg gactccaaga tccaggtttt atccaaaggg
     1921 ttgaattccg gcagcctgca ccacatcctg gacgcccaga agatgctgtg gaaccacagg
     1981 gacaggaacc tgcctcctct ggcccctctg ggcccccacc accatcacca ccattgagtc
     2041 tagacacgtg attaattaag gatcccccga cctcgacctc tggctaataa aggaaattta
     2101 ttttcattgc aatagtgtgt tggaattttt tgtgtctctc actcggaagg acatatggga
     2161 gggcaaatca tttggtcgag atccctcgga gatctctagc tagaggatcg atccccgccc
     2221 cggacgaact aaacctgact acgacatctc tgccccttct tcgcggggca gtgcatgtaa
     2281 tcccttcagt tggttggtac aacttgccaa ctgggccctg ttccacatgt gacacggggg
     2341 gggaccaaac acaaaggggt tctctgactg tagttgacat ccttataaat ggatgtgcac
     2401 atttgccaac actgagtggc tttcatcctg gagcagactt tgcagtctgt ggactgcaac
     2461 acaacattgc ctttatgtgt aactcttggc tgaagctctt acaccaatgc tgggggacat
     2521 gtacctccca ggggcccagg aagactacgg gaggctacac caacgtcaat cagaggggcc
     2581 tgtgtagcta ccgataagcg gaccctcaag agggcattag caatagtgtt tataaggccc
     2641 ccttgttaac cctaaacggg tagcatatgc ttcccgggta gtagtatata ctatccagac
     2701 taaccctaat tcaatagcat atgttaccca acgggaagca tatgctatcg aattagggtt
     2761 agtaaaaggg tcctaaggaa cagcgatatc tcccacccca tgagctgtca cggttttatt
     2821 tacatggggt caggattcca cgagggtagt gaaccatttt agtcacaagg gcagtggctg
     2881 aagatcaagg agcgggcagt gaactctcct gaatcttcgc ctgcttcttc attctccttc
     2941 gtttagctaa tagaataact gctgagttgt gaacagtaag gtgtatgtga ggtgctcgaa
     3001 aacaaggttt caggtgacgc ccccagaata aaatttggac ggggggttca gtggtggcat
     3061 tgtgctatga caccaatata accctcacaa accccttggg caataaatac tagtgtagga
     3121 atgaaacatt ctgaatatct ttaacaatag aaatccatgg ggtggggaca agccgtaaag
     3181 actggatgtc catctcacac gaatttatgg ctatgggcaa cacataatcc tagtgcaata
     3241 tgatactggg gttattaaga tgtgtcccag gcagggacca agacaggtga accatgttgt
     3301 tacactctat ttgtaacaag gggaaagaga gtggacgccg acagcagcgg actccactgg
     3361 ttgtctctaa cacccccgaa aattaaacgg ggctccacgc caatggggcc cataaacaaa
     3421 gacaagtggc cactcttttt tttgaaattg tggagtgggg gcacgcgtca gcccccacac
     3481 gccgccctgc ggttttggac tgtaaaataa gggtgtaata acttggctga ttgtaacccc
     3541 gctaaccact gcggtcaaac cacttgccca caaaaccact aatggcaccc cggggaatac
     3601 ctgcataagt aggtgggcgg gccaagatag gggcgcgatt gctgcgatct ggaggacaaa
     3661 ttacacacac ttgcgcctga gcgccaagca cagggttgtt ggtcctcata ttcacgaggt
     3721 cgctgagagc acggtgggct aatgttgcca tgggtagcat atactaccca aatatctgga
     3781 tagcatatgc tatcctaatc tatatctggg tagcataggc tatcctaatc tatatctggg
     3841 tagcatatgc tatcctaatc tatatctggg tagtatatgc tatcctaatt tatatctggg
     3901 tagcataggc tatcctaatc tatatctggg tagcatatgc tatcctaatc tatatctggg
     3961 tagtatatgc tatcctaatc tgtatccggg tagcatatgc tatcctaata gagattaggg
     4021 tagtatatgc tatcctaatt tatatctggg tagcatatac tacccaaata tctggatagc
     4081 atatgctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagc
     4141 ataggctatc ctaatctata tctgggtagc atatgctatc ctaatctata tctgggtagt
     4201 atatgctatc ctaatttata tctgggtagc ataggctatc ctaatctata tctgggtagc
     4261 atatgctatc ctaatctata tctgggtagt atatgctatc ctaatctgta tccgggtagc
     4321 atatgctatc ctcatgataa gctgtcaaac atgagaattt tcttgaagac gaaagggcct
     4381 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4441 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4501 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4561 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4621 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4681 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4741 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4801 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4861 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4921 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4981 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5041 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5101 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5161 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5221 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5281 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5341 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5401 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5461 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5521 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5581 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5641 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5701 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5761 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5821 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5881 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5941 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6001 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6061 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6121 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6181 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6241 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6301 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6361 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6421 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6481 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6541 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6601 caagctctag ctagaggtcg accaattctc atgtttgaca gcttatcatc gcagatccgg
     6661 gcaacgttgt tgccattgct gcaggcgcag aactggtagg tatggaagat ctatacattg
     6721 aatcaatatt ggcaattagc catattagtc attggttata tagcataaat caatattggc
     6781 tattggccat tgcatacgtt gtatctatat cataatatgt acatttatat tggctcatgt
     6841 ccaatatgac cgccatgttg acattgatta ttgactagtt attaatagta atcaattacg
     6901 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     6961 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     7021 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     7081 gcccacttgg cagtacatca agtgtatcat atgccaagtc cgccccctat tgacgtcaat
     7141 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttacggga ctttcctact
     7201 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     7261 accaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     7321 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaataac
     7381 cccgccccgt tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     7441 gctcgtttag tgaaccgtca gatcctcact ctcttccgca tcgctgtctg cgagggccag
     7501 ctgttgggct cgcggttgag gacaaactct tcgcggtctt tccagtactc ttggatcgga
     7561 aacccgtcgg cctccgaacg gtactccgcc accgagggac ctgagcgagt ccgcatcgac
     7621 cggatcggaa aacctctcga gaaaggcgtc taaccagtca ca
//
