LOCUS       sequence-234505-       11098 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MS2 coat protein-modified lentiviral packaging plasmid for packaging
            mRNA with an MS2 aptamer into lentivirus-like particles.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11098)
  AUTHORS   Lu B, Javidi-Parsijani P, Makani V, Mehraein-Ghomi F, Sarhan WM, Sun
            D, Yoo KW, Atala ZP, Lyu P, Atala A
  TITLE     Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for 
            transient expression and efficient genome editing.
  JOURNAL   Nucleic Acids Res. 2019 Feb 13. pii: 5316732. doi: 
            10.1093/nar/gkz093.
  PUBMED    30759231
REFERENCE   2  (bases 1 to 11098)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2019 Feb 13. pii: 5316732. doi: 10.1093/nar/gkz093."
COMMENT     Sequence Label: psPAX2-D64V-NC-MS2
FEATURES             Location/Qualifiers
     source          1..11098
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             3470..6481
                     /codon_start=1
                     /gene="pol"
                     /product="pol protein from human immunodeficiency virus 1"
                     /label=HIV-1 pol
                     /translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
                     ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
                     GIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
                     PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
                     STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
                     YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
                     DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
                     VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
                     AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
                     NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
                     LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
                     AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
                     KGIGGNEQVDKLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
                     SCDKCQLKGEAMHGQVDCSPGIWQLVCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
                     AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
                     ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
                     QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
                     MAGDDCVASRQDED"
     misc_feature    6166..6283
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7055..7288
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7473..7517
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     CDS             7811..7837
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     primer_bind     complement(8122..8141)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8187..8242
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8241..8260)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8601..8617)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8601..8617)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8614..8636)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8625..8641
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8649..8655,8656..8673,8674..8679))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8694..8715
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8774..8969
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8820..8955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    8975..9109
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9012..9031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9066..9085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9177..9194)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9348..9936)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9428..9447)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10107..10898,10899..10967))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10730..10749
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10968..11072)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg cgttgacgcg
     1741 cacggcaaga ggcgaggggc ggcgactggt gagagatggg tgcgagagcg tcagtattaa
     1801 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca
     2161 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
     2221 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga
     2281 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
     2341 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag
     2401 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga
     2461 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
     2521 ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg
     2581 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac
     2641 caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag
     2701 cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     2761 attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag
     2821 catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa gcaatgagcc
     2881 aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac caaagaaaga
     2941 ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta
     3001 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgttccggtg
     3061 gaggtggatc cggtggaggt tccatggcgt ccaatttcac gcagttcgtc ctggttgaca
     3121 acggggggac tggggacgtt acggtcgctc cgagcaactt tgccaatggt attgcggagt
     3181 ggatttcttc taattcacgg tcccaagctt acaaagtgac ctgttccgtg cggcaaagtt
     3241 ctgctcagaa tagaaagtac actataaagg tcgaagtccc taagggggcc tggcgatcat
     3301 atctcaatat ggagcttacc atcccaatat ttgccactaa ttctgattgt gaattgattg
     3361 tcaaagcaat gcaaggactc ttgaaagacg gaaacccaat ccccagcgca atcgcagcca
     3421 actccggtat atactccgga ggtggaggtg gaactgagag acaggctaat tttttaggga
     3481 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
     3541 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc
     3601 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg
     3661 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc
     3721 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg
     3781 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg
     3841 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
     3901 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc
     3961 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
     4021 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa
     4081 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac
     4141 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga
     4201 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact
     4261 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc
     4321 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
     4381 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga
     4441 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt
     4501 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
     4561 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
     4621 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
     4681 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca
     4741 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact
     4801 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat
     4861 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
     4921 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
     4981 gaaaacagga aaatatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac
     5041 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt
     5101 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac
     5161 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt
     5221 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga
     5281 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct
     5341 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc
     5401 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca
     5461 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga
     5521 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
     5581 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca
     5641 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc
     5701 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc
     5761 catgcatgga caagtagact gtagcccagg aatatggcag ctagtttgta cacatttaga
     5821 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat
     5881 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc
     5941 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc
     6001 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
     6061 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     6121 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     6181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6241 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     6301 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     6361 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
     6421 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     6481 acacatggaa ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc
     6541 agaataggcg ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga
     6601 gccctggaag catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa
     6661 gtgttgcttt cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag
     6721 gaagaagcgg agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct
     6781 atcaaagcag taagtagtac atgtaatgca acctataata gtagcaatag tagcattagt
     6841 agtagcaata ataatagcaa tagttgtgtg gtccatagta atcatagaat ataggaaaat
     6901 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6961 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7021 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7081 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7141 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7201 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7261 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7321 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7381 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7441 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7501 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7561 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7621 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7681 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     7741 gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg cggagcctgt
     7801 gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac
     7861 ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga
     7921 gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg
     7981 aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt cgccacatac
     8041 ctagaagaat aagacagggc ttggaaagga ttttgctata agctcgaaac aaccggtacc
     8101 tctagaacta tagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     8161 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     8221 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     8281 tcagaatgag tatttggttt agagtttggc aacatatgcc atatgctggc tgccatgaac
     8341 aaaggtggct ataaagaggt catcagtata tgaaacagcc ccctgctgtc cattccttat
     8401 tccatagaaa agccttgact tgaggttaga ttttttttat attttgtttt gtgttatttt
     8461 tttctttaac atccctaaaa ttttccttac atgttttact agccagattt ttcctcctct
     8521 cctgactact cccagtcata gctgtccctc ttctcttatg aagatccctc gacctgcagc
     8581 ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     8641 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
     8701 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     8761 tgccagcgga tccgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     8821 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     8881 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     8941 gcttttttgg aggcctaggc ttttgcaaaa agctaacttg tttattgcag cttataatgg
     9001 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     9061 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc gctgcattaa
     9121 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9181 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9241 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9301 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9361 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9421 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9481 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9541 caatgctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9601 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9661 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     9721 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     9781 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     9841 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     9901 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     9961 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10021 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10081 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10141 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10201 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10261 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10321 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10381 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10441 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10501 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10561 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10621 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10681 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    10741 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    10801 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    10861 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    10921 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    10981 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11041 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctg
//
