LOCUS       sequence-234589-        9045 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ectopic expression of ZIKV protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9045)
  AUTHORS   Hertzog J, Dias Junior AG, Rigby RE, Donald CL, Mayer A, Sezgin E, 
            Song C, Jin B, Hublitz P, Eggeling C, Kohl A, Rehwinkel J
  TITLE     Infection with a Brazilian isolate of Zika virus generates RIG-I 
            stimulatory RNA and the viral NS5 protein blocks type I IFN 
            induction and signaling.
  JOURNAL   Eur J Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483.
  PUBMED    29572905
REFERENCE   2  (bases 1 to 9045)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eur J 
            Immunol. 2018 Mar 23. doi: 10.1002/eji.201847483."
COMMENT     Sequence Label: pcDNA3.2/NS5-YFP
FEATURES             Location/Qualifiers
     source          1..9045
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     249..268
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        249..267
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    297..321
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(1159..1170)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     protein_bind    complement(3073..3097)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(3150..3152,3153..3665)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIE"
     primer_bind     complement(3192..3213)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3453..3472)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3800..3821
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(3871..3895)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3921..3962
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(4002..4021)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    4046..4094
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4296..4724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4383..4402)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4593..4614
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(4733..4753)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4738..5067
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4918..5053
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4980..4999
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5134..5928
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5188..5207)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     5798..5817
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6104..6225
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6141..6160)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6195..6214
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6274..6290)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6274..6290)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6287..6309)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6298..6314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6322..6328,6329..6346,6347..6352))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6367..6388
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6505..6522)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6676..7264)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6756..6775)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7435..8226,8227..8295))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8058..8077
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8296..8400)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8475..8494)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        8666..9045
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      241 atcgaaatta atacgactca ctatagggag acccaagctg gctagttaag ctatcaacaa
      301 gtttgtacaa aaaagcaggc tccgaattcg cccttaggag gacagctatg gggggtggaa
      361 caggagagac cctgggagag aaatggaagg cccgcttgaa ccagatgtcg gccctggagt
      421 tctactccta caaaaagtca ggcatcaccg aggtgtgcag agaagaggcc cgccgcgccc
      481 tcaaggatgg tgtggcaacg ggaggccatg ctgtgtcccg aggaagtgca aagctgagat
      541 ggttggtgga gcggggatac ctgcagccct atggaaaggt cattgatctt ggatgtggca
      601 gagggggctg gagttactac gccgccacca tccgcaaagt tcaagaagtg aaaggataca
      661 caaaaggagg ccctggtcat gaagaacccg tgttggtgca aagctatggg tggaacatag
      721 tccgtcttaa gagtggggtg gacgtctttc atatggcggc tgagccgtgt gacacgttgc
      781 tgtgtgacat aggtgagtca tcatctagtc ctgaagtgga agaagcacgg acgctcagag
      841 tcctctccat ggtgggggat tggcttgaaa aaagaccagg agccttttgt ataaaagtgt
      901 tgtgcccata caccagcact atgatggaaa ccctggagcg actgcagcgt aggtatgggg
      961 gaggactggt cagagtgcca ctctcccgca actctacaca tgagatgtac tgggtctctg
     1021 gagcgaaaag caacaccata aaaagtgtgt ccaccacgag ccagctcctc ttggggcgca
     1081 tggacgggcc taggaggcca gtgaaatatg aggaggatgt gaatctcggc tctggcacgc
     1141 gggctgtggt aagctgcgct gaagctccca acatgaagat cattggtaac cgcattgaaa
     1201 ggatccgcag tgagcacgcg gaaacgtggt tctttgacga gaaccaccca tataggacat
     1261 gggcttacca tggaagctat gaggccccca cacaagggtc agcgtcctct ctaataaacg
     1321 gggttgtcag gctcctgtca aaaccctggg atgtggtgac tggagtcaca ggaatagcca
     1381 tgaccgacac cacaccgtat ggtcagcaaa gagttttcaa ggaaaaagtg gacactaggg
     1441 tgccagaccc ccaagaaggc actcgtcagg ttatgagcat ggtctcttcc tggttgtgga
     1501 aagagctagg caaacacaaa cggccacgag tctgtaccaa agaagagttc atcaacaagg
     1561 ttcgtagcaa tgcagcatta ggggcaatat ttgaagagga aaaagagtgg aagactgcag
     1621 tggaagctgt gaacgatcca aggttctggg ctctagtgga caaggaaaga gagcaccacc
     1681 tgagaggaga gtgccagagt tgtgtgtaca acatgatggg aaaaagagaa aagaaacaag
     1741 gggaatttgg aaaggccaag ggcagccgcg ccatctggta tatgtggcta ggggctagat
     1801 ttctagagtt cgaagccctt ggattcttga acgaggatca ctggatgggg agagagaact
     1861 caggaggtgg tgttgaaggg ctgggattac aaagactcgg atatgtccta gaagagatga
     1921 gtcgcatacc aggaggaagg atgtatgcag atgacactgc tggctgggac acccgcatca
     1981 gcaggttcga tctggagaat gaagctctaa tcaccaacca aatggagaaa gggcataggg
     2041 ccttggcatt ggccataatc aagtacacat accaaaacaa agtggtaaag gtccttagac
     2101 cagctgaaaa agggaaaaca gttatggaca ttatttcgag acaagaccaa agggggagcg
     2161 gacaagttgt cacttacgct cttaacacat ttaccaacct agtggtgcaa ctcattcgga
     2221 atatggaggc tgaggaagtt ctagagatgc aagacttgtg gctgctgcgg aggtcagaga
     2281 aagtgaccaa ctggttgcag agcaacggat gggataggct caaacgaatg gcagtcagtg
     2341 gagatgattg cgttgtgaag ccaattgatg ataggtttgc acatgccctc aggttcttga
     2401 atgatatggg aaaagttagg aaggacacac aagagtggaa accctcaact ggatgggaca
     2461 actgggaaga agttccgttt tgctcccacc acttcaacaa gctccatctc aaggacggga
     2521 ggtccattgt ggttccctgc cgccaccaag atgaactgat tggccgggcc cgcgtctctc
     2581 caggggcggg atggagcatc cgggagactg cttgcctagc aaaatcatat gcgcaaatgt
     2641 ggcagctcct ttatttccac agaagggacc tccgactgat ggccaatgcc atttgttcat
     2701 ctgtgccagt tgactgggtt ccaactggga gaactacctg gtcaatccat ggaaagggag
     2761 aatggatgac cactgaagac atgcttgtgg tgtggaacag agtgtggatt gaggagaacg
     2821 accacatgga agacaagacc ccagttacga aatggacaga cattccctat ttgggaaaaa
     2881 gggaagactt gtggtgtgga tctctcatag ggcacagacc gcgcaccacc tgggctgaga
     2941 acattaaaaa cacagtcaac atggtgcgca ggatcatagg tgatgaagaa aagtacatgg
     3001 actacctatc cacccaagtt cgctacttgg gtgaagaagg gtctacacct ggagtgctga
     3061 agggcgaatt cgacccagct ttcttgtaca aagtggttga tatccagcac agtggcggcc
     3121 gctcgagtct agaggggccg cggttcgaag tgagcaaggg cgaggagctg ttcaccgggg
     3181 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     3241 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     3301 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgcagtgct
     3361 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     3421 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     3481 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     3541 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     3601 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     3661 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3721 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     3781 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     3841 tcggcatgga cgagctgaag ggcgaattcg acccagcttt cttgtacaaa gtggttgatc
     3901 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
     3961 cgcgtaccgg ttagtaatga gtttaaacgg gggaggctaa ctgaaacacg gaaggagaca
     4021 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacgggtgt
     4081 tgggtcgttt gttcataaac gcggggttcg gtcccagggc tggcactctg tcgatacccc
     4141 accgagaccc cattggggcc aatacgcccg cgtttcttcc ttttccccac cccacccccc
     4201 aagttcgggt gaaggcccag ggctcgcagc caacgtcggg gcggcaggcc ctgccatagc
     4261 agatctgcgc agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag
     4321 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     4381 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     4441 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     4501 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     4561 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     4621 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     4681 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg
     4741 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     4801 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     4861 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     4921 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     4981 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc
     5041 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga
     5101 tctgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca
     5161 ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc
     5221 ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc
     5281 aagaccgacc tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg
     5341 ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg
     5401 gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct
     5461 gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct
     5521 acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa
     5581 gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa
     5641 ctgttcgcca ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc
     5701 gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt
     5761 ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct
     5821 gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc
     5881 gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg
     5941 ggttcgcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac
     6001 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat
     6061 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc
     6121 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     6181 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc
     6241 gtcgacctct agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     6301 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
     6361 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
     6421 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt
     6481 gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6541 gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga
     6601 taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc
     6661 cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg
     6721 ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg
     6781 aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt
     6841 tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt
     6901 gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg
     6961 cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact
     7021 ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt
     7081 cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct
     7141 gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac
     7201 cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc
     7261 tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg
     7321 ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta
     7381 aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     7441 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     7501 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     7561 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     7621 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     7681 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     7741 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     7801 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     7861 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7921 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7981 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     8041 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     8101 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     8161 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     8221 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     8281 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     8341 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     8401 cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta
     8461 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct
     8521 gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa
     8581 ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc
     8641 gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca
     8701 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8761 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8821 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8881 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8941 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     9001 cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
