LOCUS       sequence-234769-        6894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of recombinant S. mansoni protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6894)
  AUTHORS   Cecile Crosnier, Anna V. Protasio, Gabriel Rinaldi, Shona Wilson, 
            Matthew Berriman, Gavin J. Wright
  TITLE     A library of cell-surface and secreted proteins from Schistosoma 
            mansoni identifies early serological markers of infection
  JOURNAL   bioRXiv
REFERENCE   2  (bases 1 to 6894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRXiv"
COMMENT     Sequence Label: Smp_201730-Cd4-bio-His
FEATURES             Location/Qualifiers
     source          1..6894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..204
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     155..175
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     201..225
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     706..725
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      748..757
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1672..1689
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    1741..1796
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(1795..1814)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4032..4050)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4118..4222
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4223..4291,4292..5083)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4441..4460)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5254..5842
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5743..5762
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5996..6013
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6130..6151
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6166..6171,6172..6189,6190..6196)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6204..6220
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6209..6231
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6228..6244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6228..6244
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        6515..6894
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 gtgatgcggt tttggcagta caccaatggg cgtggatagc ggtttgactc acggggattt
       61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      121 tttccaaaat gtcgtaataa ccccgccccg ttgacgcaaa tgggcggtag gcgtgtacgg
      181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcctcac tctcttccgc
      241 atcgctgtct gcgagggcca gctgttgggc tcgcggttga ggacaaactc ttcgcggtct
      301 ttccagtact cttggatcgg aaacccgtcg gcctccgaac ggtactccgc caccgaggga
      361 cctgagcgag tccgcatcga ccggatcgga aaacctctcg agaaaggcgt ctaaccagtc
      421 acagtcgcaa ggtaggctga gcaccgtggc gggcggcagc gggtggcggt cggggttgtt
      481 tctggcggag gtgctgctga tgatgtaatt aaagtaggcg gtcttgagac ggcggatggt
      541 cgaggtgagg tgtggcaggc ttgagatcca gctgttgggg tgagtactcc ctctcaaaag
      601 cgggcattac ttctgcgcta agattgtcag tttccaaaaa cgaggaggat ttgatattca
      661 cctggcccga tctggccata cacttgagtg acaatgacat ccactttgcc tttctctcca
      721 caggtgtcca ctcccaggtc caagtttgcc accatggagt ttcagaccca ggtactcatg
      781 tccctgctgc tctgcatgtc tggtgcggcc gccgagtttt ggagagagaa caacctgccc
      841 tgcaagaacc tgggcacctt ctgccggaag ctgcccttca gccagcggtg ctgcgacacc
      901 accgtgtgcg acctgaaggc ccccttcagc ggcacctgtg tgcggtgcct ggacaagggc
      961 cggttctgcc tgcggagcag agagtgctgc gataagtact gcttcttctt caaatgccgg
     1021 ggcgcgccgt cgacctccat cacggcctat aagagtgagg gggagtcagc ggagttctcc
     1081 ttcccactca accttggaga ggaaagcctg cagggagagt tgagatggaa ggcagagaag
     1141 gctccttctt cccagtcctg gatcaccttc tccctaaaga accaaaaggt gtctgtgcag
     1201 aagtctacta gcaaccccaa gttccagctg tccgaaacgc tcccactcac ccttcagata
     1261 ccccaggtct cccttcagtt tgctggttct ggcaacctga ccctgactct ggacagaggg
     1321 atactgtatc aggaagtgaa cctggtggtg atgaaagtga ctcagcccga cagcaacact
     1381 ttgacctgtg aggtgatggg acccacctca cccaagatga gactgatctt gaagcaggag
     1441 aatcaggagg ccagggtctc caggcaggag aaagtgattc aagtgcaggc ccctgaagca
     1501 ggggtgtggc aatgtctact gagtgaaggt gaagaggtca agatggactc caagatccag
     1561 gttttatcca aagggttgaa ttccggcagc ctgcaccaca tcctggacgc ccagaagatg
     1621 ctgtggaacc acagggacag gaacctgcct cctctggccc ctctgggccc ccaccaccat
     1681 caccaccatt gagtctagac acgtgattaa ttaaggatcc cccgacctcg acctctggct
     1741 aataaaggaa atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg
     1801 gaaggacata tgggagggca aatcatttgg tcgagatccc tcggagatct ctagctagag
     1861 gatcgatccc cgccccggac gaactaaacc tgactacgac atctctgccc cttcttcgcg
     1921 gggcagtgca tgtaatccct tcagttggtt ggtacaactt gccaactggg ccctgttcca
     1981 catgtgacac ggggggggac caaacacaaa ggggttctct gactgtagtt gacatcctta
     2041 taaatggatg tgcacatttg ccaacactga gtggctttca tcctggagca gactttgcag
     2101 tctgtggact gcaacacaac attgccttta tgtgtaactc ttggctgaag ctcttacacc
     2161 aatgctgggg gacatgtacc tcccaggggc ccaggaagac tacgggaggc tacaccaacg
     2221 tcaatcagag gggcctgtgt agctaccgat aagcggaccc tcaagagggc attagcaata
     2281 gtgtttataa ggcccccttg ttaaccctaa acgggtagca tatgcttccc gggtagtagt
     2341 atatactatc cagactaacc ctaattcaat agcatatgtt acccaacggg aagcatatgc
     2401 tatcgaatta gggttagtaa aagggtccta aggaacagcg atatctccca ccccatgagc
     2461 tgtcacggtt ttatttacat ggggtcagga ttccacgagg gtagtgaacc attttagtca
     2521 caagggcagt ggctgaagat caaggagcgg gcagtgaact ctcctgaatc ttcgcctgct
     2581 tcttcattct ccttcgttta gctaatagaa taactgctga gttgtgaaca gtaaggtgta
     2641 tgtgaggtgc tcgaaaacaa ggtttcaggt gacgccccca gaataaaatt tggacggggg
     2701 gttcagtggt ggcattgtgc tatgacacca atataaccct cacaaacccc ttgggcaata
     2761 aatactagtg taggaatgaa acattctgaa tatctttaac aatagaaatc catggggtgg
     2821 ggacaagccg taaagactgg atgtccatct cacacgaatt tatggctatg ggcaacacat
     2881 aatcctagtg caatatgata ctggggttat taagatgtgt cccaggcagg gaccaagaca
     2941 ggtgaaccat gttgttacac tctatttgta acaaggggaa agagagtgga cgccgacagc
     3001 agcggactcc actggttgtc tctaacaccc ccgaaaatta aacggggctc cacgccaatg
     3061 gggcccataa acaaagacaa gtggccactc ttttttttga aattgtggag tgggggcacg
     3121 cgtcagcccc cacacgccgc cctgcggttt tggactgtaa aataagggtg taataacttg
     3181 gctgattgta accccgctaa ccactgcggt caaaccactt gcccacaaaa ccactaatgg
     3241 caccccgggg aatacctgca taagtaggtg ggcgggccaa gataggggcg cgattgctgc
     3301 gatctggagg acaaattaca cacacttgcg cctgagcgcc aagcacaggg ttgttggtcc
     3361 tcatattcac gaggtcgctg agagcacggt gggctaatgt tgccatgggt agcatatact
     3421 acccaaatat ctggatagca tatgctatcc taatctatat ctgggtagca taggctatcc
     3481 taatctatat ctgggtagca tatgctatcc taatctatat ctgggtagta tatgctatcc
     3541 taatttatat ctgggtagca taggctatcc taatctatat ctgggtagca tatgctatcc
     3601 taatctatat ctgggtagta tatgctatcc taatctgtat ccgggtagca tatgctatcc
     3661 taatagagat tagggtagta tatgctatcc taatttatat ctgggtagca tatactaccc
     3721 aaatatctgg atagcatatg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     3781 ctatatctgg gtagcatagg ctatcctaat ctatatctgg gtagcatatg ctatcctaat
     3841 ctatatctgg gtagtatatg ctatcctaat ttatatctgg gtagcatagg ctatcctaat
     3901 ctatatctgg gtagcatatg ctatcctaat ctatatctgg gtagtatatg ctatcctaat
     3961 ctgtatccgg gtagcatatg ctatcctcat gataagctgt caaacatgag aattttcttg
     4021 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
     4081 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     4141 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     4201 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     4261 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     4321 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     4381 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     4441 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat
     4501 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     4561 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     4621 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     4681 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     4741 cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac
     4801 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     4861 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     4921 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     4981 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5041 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     5101 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     5161 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     5221 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     5281 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     5341 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     5401 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     5461 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     5521 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     5581 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     5641 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     5701 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     5761 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     5821 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     5881 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     5941 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6001 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6061 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     6121 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     6181 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     6241 tgaccatgat tacgccaagc tctagctaga ggtcgaccaa ttctcatgtt tgacagctta
     6301 tcatcgcaga tccgggcaac gttgttgcca ttgctgcagg cgcagaactg gtaggtatgg
     6361 aagatctata cattgaatca atattggcaa ttagccatat tagtcattgg ttatatagca
     6421 taaatcaata ttggctattg gccattgcat acgttgtatc tatatcataa tatgtacatt
     6481 tatattggct catgtccaat atgaccgcca tgttgacatt gattattgac tagttattaa
     6541 tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg cgttacataa
     6601 cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata
     6661 atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag
     6721 tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc aagtccgccc
     6781 cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta
     6841 cgggactttc ctacttggca gtacatctac gtattagtca tcgctattac catg
//
