LOCUS       sequence-234910-        9402 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of GFP tagged CD274 with its own 3'UTR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9402)
  AUTHORS   Ma W, Mayr C
  TITLE     A Membraneless Organelle Associated with the Endoplasmic Reticulum 
            Enables 3'UTR-Mediated Protein-Protein Interactions.
  JOURNAL   Cell. 2018 Oct 30. pii: S0092-8674(18)31315-1. doi: 
            10.1016/j.cell.2018.10.007.
  PUBMED    30449617
REFERENCE   2  (bases 1 to 9402)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2018 
            Oct 30. pii: S0092-8674(18)31315-1. doi: 
            10.1016/j.cell.2018.10.007."
COMMENT     Sequence Label: SP-GFP-CD274-UTR
FEATURES             Location/Qualifiers
     source          1..9402
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(961..963,964..966,967..1677)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1006..1027)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1267..1286)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1614..1635
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(4232..4243)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     primer_bind     complement(5242..5259)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5248..5472
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5518..5946
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5605..5624)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5815..5836
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5955..5975)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5960..6289
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6140..6275
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6202..6221
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             6302..6901
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6302..6321)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6798..6818
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    7075..7196
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7112..7131)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7166..7185
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7245..7261)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7245..7261)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7258..7280)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7269..7285
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7293..7299,7300..7317,7318..7323))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7338..7359
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7476..7493)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7647..8235)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7727..7746)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8406..9197,9198..9266))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9029..9048
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9267..9371)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatga ggatatttgc tgtctttata ttcatgacct actggcattt gctgaacgca
      961 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
     1021 ggcgacgtaa acggccacaa gttcagcgtg tctggcgagg gcgagggcga tgccacctac
     1081 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
     1141 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
     1201 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
     1261 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
     1321 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
     1381 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
     1441 ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
     1501 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
     1561 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
     1621 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagttt
     1681 actgtcacgg ttcccaagga cctatatgtg gtagagtatg gtagcaatat gacaattgaa
     1741 tgcaaattcc cagtagaaaa acaattagac ctggctgcac taattgtcta ttgggaaatg
     1801 gaggataaga acattattca atttgtgcat ggagaggaag acctgaaggt tcagcatagt
     1861 agctacagac agagggcccg gctgttgaag gaccagctct ccctgggaaa tgctgcactt
     1921 cagatcacag atgtgaaatt gcaggatgca ggggtgtacc gctgcatgat cagctatggt
     1981 ggtgccgact acaagcgaat tactgtgaaa gtcaatgccc catacaacaa aatcaaccaa
     2041 agaattttgg ttgtggatcc agtcacctct gaacatgaac tgacatgtca ggctgagggc
     2101 taccccaagg ccgaagtcat ctggacaagc agtgaccatc aagtcctgag tggtaagacc
     2161 accaccacca attccaagag agaggagaag cttttcaatg tgaccagcac actgagaatc
     2221 aacacaacaa ctaatgagat tttctactgc acttttagga gattagatcc tgaggaaaac
     2281 catacagctg aattggtcat cccagaacta cctctggcac atcctccaaa tgaaaggact
     2341 cacttggtaa ttctgggagc catcttatta tgccttggtg tagcactgac attcatcttc
     2401 cgtttaagaa aagggagaat gatggatgtg aaaaaatgtg gcatccaaga tacaaactca
     2461 aagaagcaaa gtgatacaca tttggaggag acgtaaggcg gccgctccag cattggaact
     2521 tctgatcttc aagcagggat tctcaacctg tggtttaggg gttcatcggg gctgagcgtg
     2581 acaagaggaa ggaatggacc cgtgggatgc aggcaatgtg ggacttaaaa ggcccaagca
     2641 ctgaaaatgg aacctggcga aagcagagga ggagaatgaa gaaagatgga gtcaaacagg
     2701 gagcctggag ggagaccttg atactttcaa atgcctgagg ggctcatcga cgcctgtgac
     2761 agggagaaag gatacttctg aacaaggagc ctccaagcaa atcatccatt gctcatccta
     2821 ggaagacggg ttgagaatcc ctaatttgag ggtcagttcc tgcagaagtg ccctttgcct
     2881 ccactcaatg cctcaatttc ttttctgcat gactgagagt ctcagtgttg gaacgggaca
     2941 gtatttatgt atgagttttt cctatttatt ttgagtctgt gaggtcttct tgtcatgtga
     3001 gtgtggttgt gaatgatttc ttttgaagat atattgtagt agatgttaca attttgtcgc
     3061 caaactaaac ttgctgctta atgatttgct cacatctagt aaaacatgga gtatttgtaa
     3121 ggtgcttggt ctcctctata actacaagta tacattggaa gcataaagat caaaccgttg
     3181 gttgcatagg atgtcacctt tatttaaccc attaatactc tggttgacct aatcttattc
     3241 tcagacctca agtgtctgtg cagtatctgt tccatttaaa tatcagcttt acaattatgt
     3301 ggtagcctac acacataatc tcatttcatc gctgtaacca ccctgttgtg ataaccacta
     3361 ttattttacc catcgtacag ctgaggaagc aaacagatta agtaacttgc ccaaaccagt
     3421 aaatagcaga cctcagactg ccacccactg tccttttata atacaattta cagctatatt
     3481 ttactttaag caattctttt attcaaaaac catttattaa gtgcccttgc aatatcaatc
     3541 gctgtgccag gcattgaatc tacagatgtg agcaagacaa agtacctgtc ctcaaggagc
     3601 tcatagtata atgaggagat taacaagaaa atgtattatt acaatttagt ccagtgtcat
     3661 agcataagga tgatgcgagg ggaaaacccg agcagtgttg ccaagaggag gaaataggcc
     3721 aatgtggtct gggacggttg gatatactta aacatcttaa taatcagagt aattttcatt
     3781 tacaaagaga ggtcggtact taaaataacc ctgaaaaata acactggaat tccttttcta
     3841 gcattatatt tattcctgat ttgcctttgc catataatct aatgcttgtt tatatagtgt
     3901 ctggtattgt ttaacagttc tgtcttttct atttaaatgc cactaaattt taaattcata
     3961 cctttccatg attcaaaatt caaaagatcc catgggagat ggttggaaaa tctccacttc
     4021 atcctccaag ccattcaagt ttcctttcca gaagcaactg ctactgcctt tcattcatat
     4081 gttcttctaa agatagtcta catttggaaa tgtatgttaa aagcacgtat ttttaaaatt
     4141 tttttcctaa atagtaacac attgtatgtc tgctgtgtac tttgctattt ttatttattt
     4201 tagtgtttct tatatagcag atggaatgaa tttgaagttc ccagggctga ggatccatgc
     4261 cttctttgtt tctaagttat ctttcccata gcttttcatt atctttcata tgatccagta
     4321 tatgttaaat atgtcctaca tatacattta gacaaccacc atttgttaag tatttgctct
     4381 aggacagagt ttggatttgt ttatgtttgc tcaaaaggag acccatgggc tctccagggt
     4441 gcactgagtc aatctagtcc taaaaagcaa tcttattatt aactctgtat gacagaatca
     4501 tgtctggaac ttttgttttc tgctttctgt caagtataaa cttcactttg atgctgtact
     4561 tgcaaaatca cattttcttt ctggaaattc cggcagtgta ccttgactgc tagctaccct
     4621 gtgccagaaa agcctcattc gttgtgcttg aacccttgaa tgccaccagc tgtcatcact
     4681 acacagccct cctaagaggc ttcctggagg tttcgagatt cagatgccct gggagatccc
     4741 agagtttcct ttccctcttg gccatattct ggtgtcaatg acaaggagta ccttggcttt
     4801 gccacatgtc aaggctgaag aaacagtgtc tccaacagag ctccttgtgt tatctgtttg
     4861 tacatgtgca tttgtacagt aattggtgtg acagtgttct ttgtgtgaat tacaggcaag
     4921 aattgtggct gagcaaggca catagtctac tcagtctatt cctaagtcct aactcctcct
     4981 tgtggtgttg gatttgtaag gcactttatc ccttttgtct catgtttcat cgtaaatggc
     5041 ataggcagag atgataccta attctgcatt tgattgtcac tttttgtacc tgcattaatt
     5101 taataaaata ttcttattta ttttgttact tggtacaccg gcatgtccat tttcttgttt
     5161 attttgtgtt taataaaatg ttcagtttaa catcccagtg gagaaagtta tctagagggc
     5221 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     5281 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     5341 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     5401 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
     5461 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
     5521 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
     5581 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
     5641 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
     5701 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
     5761 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
     5821 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
     5881 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
     5941 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
     6001 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
     6061 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
     6121 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     6181 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
     6241 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccaac
     6301 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     6361 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     6421 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     6481 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     6541 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     6601 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     6661 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     6721 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     6781 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     6841 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     6901 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
     6961 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7021 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7081 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7141 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7201 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7261 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7321 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7381 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7441 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7501 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7561 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7621 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7681 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7741 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7801 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7861 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7921 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7981 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8041 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8101 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8161 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8221 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8281 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8341 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8401 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8461 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8521 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8581 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8641 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8701 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8761 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8821 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8881 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8941 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9001 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9061 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9121 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9181 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9241 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9301 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9361 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tc
//
