LOCUS       sequence-234949-        8797 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Open reading frame vector encoding HRAS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8797)
  AUTHORS   Hong AL, Guerriero JL, Doshi MB, Kynnap BD, Kim WJ, Schinzel AC, 
            Modiste R, Schlauch AJ, Adam RM, Kwiatkowski DJ, Beroukhim R, Letai 
            A, Rosenberg JE, Hahn WC
  TITLE     MCL1 and DEDD promote urothelial carcinoma progression.
  JOURNAL   Mol Cancer Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963.
  PUBMED    30777879
REFERENCE   2  (bases 1 to 8797)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cancer 
            Res. 2019 Feb 18. pii: 1541-7786.MCR-18-0963. doi: 
            10.1158/1541-7786.MCR-18-0963."
COMMENT     Sequence Label: pLX311 HRAS
FEATURES             Location/Qualifiers
     source          1..8797
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        23..219
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             241..636
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    710..827
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        965..2143
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          1196..2134
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     2091..2111
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    2163..2187
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             join(2190..2222,2223..2225,2226..2759)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(2761..2785)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2793..2834
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2885..3473
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2938..2958)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3356..3367)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3545..3778
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3856..3977
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3893..3912)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3947..3966
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4017..4152
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4172..4191)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4173..4191)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4201..4218)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4201..4217)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4210..4232)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4359..4814
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4446..4465)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4656..4677
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4840..4944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4945..5013,5014..5805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5163..5182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5976..6564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6465..6484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6718..6735
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6852..6873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6888..6893,6894..6911,6912..6918)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6926..6942
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6931..6953
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6950..6966
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6950..6966
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6985..7005
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6987..7005
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7033..7259
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7260..7440
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7487..7612
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8105..8338
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8523..8567
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcgtctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctttctag aggtaccacc
      241 atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac ggctacaatc
      301 aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc tagcgacggc
      361 cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc agaactcgtg
      421 gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt cgcgatcgga
      481 aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct tctcgatctg
      541 catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc agttgggatt
      601 cgtgaattgc tgccctctgg ttatgtgtgg gagggcctgc agctgcagta gtaaggcgcg
      661 ccgttaacga attctagatc ttgagacaaa tggcagtatt catccacaat tttaaaagaa
      721 aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca
      781 tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca
      841 gggacagcag agatccactt tggcgccggc tcgaggcctg caggtgcaaa gatggataaa
      901 gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg
      961 aattggctcc ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt
     1021 ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga
     1081 aagtgatgtc gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag
     1141 tgcagtagtc gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag
     1201 tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg
     1261 aattacttcc acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg
     1321 gtgggagagt tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc
     1381 ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc
     1441 tgctttcgat aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt
     1501 ctggcaagat agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg
     1561 ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct
     1621 gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt
     1681 gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc
     1741 accagttgcg tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg
     1801 gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt
     1861 tccgtcctca gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct
     1921 cgattagttc tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc
     1981 gatggagttt ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat
     2041 gtaattctcc ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca
     2101 gacagtggtt caaagttttt ttcttccatt tcaggtgtcg tgaggctagc atcgattgat
     2161 caacaagttt gtacaaaaaa gttggcacca tgacggaata taagctggtg gtggtgggcg
     2221 ccgtcggtgt gggcaagagt gcgctgacca tccagctgat ccagaaccat tttgtggacg
     2281 aatacgaccc cactatagag gattcctacc ggaagcaggt ggtcattgat ggggagacgt
     2341 gcctgttgga catcctggat accgccggcc aggaggagta cagcgccatg cgggaccagt
     2401 acatgcgcac cggggagggc ttcctgtgtg tgtttgccat caacaacacc aagtcttttg
     2461 aggacatcca ccagtacagg gagcagatca aacgggtgaa ggactcggat gacgtgccca
     2521 tggtgctggt ggggaacaag tgtgacctgg ctgcacgcac tgtggaatct cggcaggctc
     2581 aggacctcgc ccgaagctac ggcatcccct acatcgagac ctcggccaag acccggcagg
     2641 gagtggagga tgccttctac acgttggtgc gtgagatccg gcagcacaag ctgcggaagc
     2701 tgaaccctcc tgatgagagt ggccccggct gcatgagctg caagtgtgtg ctctcctgat
     2761 tgccaacttt cttgtacaaa gtggttgata tcggtaagcc tatccctaac cctctcctcg
     2821 gtctcgattc tacgtagtaa tgaactagta ccggttaagt cgacaatcaa cgcgttaagt
     2881 cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2941 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3001 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3061 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3121 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3181 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3241 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     3301 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     3361 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3421 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact
     3481 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     3541 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg
     3601 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     3661 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     3721 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     3781 acgtatagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     3841 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     3901 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     3961 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     4021 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     4081 tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     4141 tttttggagg cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc
     4201 actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     4261 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     4321 cccttcccaa cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt
     4381 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     4441 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     4501 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     4561 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     4621 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     4681 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     4741 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     4801 aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     4861 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     4921 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     4981 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5041 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5101 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5161 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     5221 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5281 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5341 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5401 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5461 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     5521 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5581 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5641 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5701 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     5761 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     5821 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     5881 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     5941 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6001 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6061 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6121 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6181 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6241 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6301 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6361 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6421 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6481 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6541 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6601 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6661 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6721 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     6781 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     6841 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     6901 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     6961 tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag
     7021 ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga
     7081 gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa
     7141 ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa
     7201 ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg
     7261 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7321 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7381 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7441 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
     7501 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
     7561 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
     7621 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
     7681 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
     7741 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
     7801 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
     7861 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
     7921 aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag
     7981 atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat
     8041 taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg
     8101 gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt
     8161 caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca
     8221 atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca
     8281 agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg
     8341 ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt
     8401 ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag
     8461 aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag
     8521 aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta
     8581 acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag
     8641 gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac
     8701 cattatcgtt tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta
     8761 gtcagcaacc aggtgtggaa agtccccagg ctcccca
//
