LOCUS       sequence-235075-       10708 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  encodes a GAG (F-MLV)-CAS9(sp) fusion. Allows the production of 
            GAG-CAS9 Virus like particles from producer cells in association 
            with over expressed gRNA(s) and appropriate envelopes..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10708)
  AUTHORS   Mangeot PE, Risson V, Fusil F, Marnef A, Laurent E, Blin J, 
            Mournetas V, Massourides E, Sohier TJM, Corbin A, Aube F, Teixeira 
            M, Pinset C, Schaeffer L, Legube G, Cosset FL, Verhoeyen E, Ohlmann 
            T, Ricci EP
  TITLE     Genome editing in primary cells and in vivo using viral-derived 
            Nanoblades loaded with Cas9-sgRNA ribonucleoproteins.
  JOURNAL   Nat Commun. 2019 Jan 3;10(1):45. doi: 10.1038/s41467-018-07845-z.
  PUBMED    30604748
REFERENCE   2  (bases 1 to 10708)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2019-01-3"; volume: "10(1):45. doi"; pages: " 
            10.1038/s41467-018-07845-z"
COMMENT     Sequence Label: BIC-Gag-CAS9
FEATURES             Location/Qualifiers
     source          1..10708
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1313..1336
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1343..1363
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1388..5488
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             1388..5488
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5489..5536
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     primer_bind     complement(5626..5645)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5691..5746
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5745..5764)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(6104..6120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6104..6120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6117..6139)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6128..6144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6152..6158,6159..6176,6177..6182))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6197..6218
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6335..6352)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6506..7094)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6586..6605)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7265..8056,8057..8125))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7888..7907
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8126..8230)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(8256..8711)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8393..8414)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8605..8624
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8838..8860
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8852..8869
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8853..8869
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8879..8898
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8879..8897
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        9018..9397
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9398..9601
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9552..9572
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9598..9622
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          9713..10285
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
ORIGIN
        1 cccttcagcc ccctctctcc cacctgaacc cccactctcg accccgcccc agtcctccct
       61 ctatccggct ctcacttctc ctttaaacac caaacctagg cctcaagtcc ttcctgatag
      121 cggaggacca ctcattgatc tactcacgga ggaccctccg ccttaccggg acccagggcc
      181 accctctcct gacgggaacg gcgatagcgg agaagtggcc cctacagaag gagcccctga
      241 cccttcccca atggtatccc gcctgcgggg aagaaaagaa ccccccgtgg cggattctac
      301 tacctctcag gcgttccccc ttcgcctggg agggaatgga cagtatcaat actggccatt
      361 ttcctcctct gacctctata actggaaaaa taacaacccc tctttctccg aggacccagc
      421 taaattgaca gctttgatcg agtccgttct ccttactcat cagcccactt gggatgactg
      481 ccaacagcta ttagggaccc tgctgacggg agaagaaaaa cagcgagtgc tcctagaggc
      541 ccgaaaggcg gttcgagggg aggacggacg cccaactcag ctgcccaatg acattaatga
      601 tgcttttccc ttggaacgtc ccgactggga ctacaacacc caacgaggta ggaaccacct
      661 agtccactat cgccagttgc tcctagcggg tctccaaaac gcgggcagaa gccccaccaa
      721 tttggccaag gtaaaaggga taacccaggg acctaatgag tctccctcag cctttttaga
      781 gagactcaag gaggcctatc gcagatacac tccttatgac cctgaggacc cagggcaaga
      841 aaccaatgtg gccatgtcat tcatctggca gtccgccccg gatatcgggc gaaagttaga
      901 gcggttagaa gatttgaaga gtaagacctt aggagactta gtgagggaag ctgaaaagat
      961 ctttaataaa cgagaaaccc cggaagaaag agaggaacgt attaggagag aaacagagga
     1021 aaaggaagaa cgccgtaggg cagaggatgt gcagagagag aaggagaggg accgcagaag
     1081 acatagagaa atgagtaagt tgctggctac tgtcgttagc gggcagagac aggatagaca
     1141 gggaggagag cgaaggaggc cccaactcga ccacgaccag tgtgcctact gcaaagaaaa
     1201 gggacattgg gctagagatt gccccaagaa gccaagagga ccccggggac cacgacccca
     1261 ggcctccctc ctgacgcgta gttccctgta tccagccctc acacctaccg gtgattacaa
     1321 agacgatgac gataagatgg ccccaaagaa gaagcggaag gtcggtatcc acggagtccc
     1381 agcagccgac aagaagtaca gcatcggcct ggacatcggc accaactctg tgggctgggc
     1441 cgtgatcacc gacgagtaca aggtgcccag caagaaattc aaggtgctgg gcaacaccga
     1501 ccggcacagc atcaagaaga acctgatcgg agccctgctg ttcgacagcg gcgaaacagc
     1561 cgaggccacc cggctgaaga gaaccgccag aagaagatac accagacgga agaaccggat
     1621 ctgctatctg caagagatct tcagcaacga gatggccaag gtggacgaca gcttcttcca
     1681 cagactggaa gagtccttcc tggtggaaga ggataagaag cacgagcggc accccatctt
     1741 cggcaacatc gtggacgagg tggcctacca cgagaagtac cccaccatct accacctgag
     1801 aaagaaactg gtggacagca ccgacaaggc cgacctgcgg ctgatctatc tggccctggc
     1861 ccacatgatc aagttccggg gccacttcct gatcgagggc gacctgaacc ccgacaacag
     1921 cgacgtggac aagctgttca tccagctggt gcagacctac aaccagctgt tcgaggaaaa
     1981 ccccatcaac gccagcggcg tggacgccaa ggccatcctg tctgccagac tgagcaagag
     2041 cagacggctg gaaaatctga tcgcccagct gcccggcgag aagaagaatg gcctgttcgg
     2101 aaacctgatt gccctgagcc tgggcctgac ccccaacttc aagagcaact tcgacctggc
     2161 cgaggatgcc aaactgcagc tgagcaagga cacctacgac gacgacctgg acaacctgct
     2221 ggcccagatc ggcgaccagt acgccgacct gtttctggcc gccaagaacc tgtccgacgc
     2281 catcctgctg agcgacatcc tgagagtgaa caccgagatc accaaggccc ccctgagcgc
     2341 ctctatgatc aagagatacg acgagcacca ccaggacctg accctgctga aagctctcgt
     2401 gcggcagcag ctgcctgaga agtacaaaga gattttcttc gaccagagca agaacggcta
     2461 cgccggctac attgacggcg gagccagcca ggaagagttc tacaagttca tcaagcccat
     2521 cctggaaaag atggacggca ccgaggaact gctcgtgaag ctgaacagag aggacctgct
     2581 gcggaagcag cggaccttcg acaacggcag catcccccac cagatccacc tgggagagct
     2641 gcacgccatt ctgcggcggc aggaagattt ttacccattc ctgaaggaca accgggaaaa
     2701 gatcgagaag atcctgacct tccgcatccc ctactacgtg ggccctctgg ccaggggaaa
     2761 cagcagattc gcctggatga ccagaaagag cgaggaaacc atcaccccct ggaacttcga
     2821 ggaagtggtg gacaagggcg cttccgccca gagcttcatc gagcggatga ccaacttcga
     2881 taagaacctg cccaacgaga aggtgctgcc caagcacagc ctgctgtacg agtacttcac
     2941 cgtgtataac gagctgacca aagtgaaata cgtgaccgag ggaatgagaa agcccgcctt
     3001 cctgagcggc gagcagaaaa aggccatcgt ggacctgctg ttcaagacca accggaaagt
     3061 gaccgtgaag cagctgaaag aggactactt caagaaaatc gagtgcttcg actccgtgga
     3121 aatctccggc gtggaagatc ggttcaacgc ctccctgggc acataccacg atctgctgaa
     3181 aattatcaag gacaaggact tcctggacaa tgaggaaaac gaggacattc tggaagatat
     3241 cgtgctgacc ctgacactgt ttgaggacag agagatgatc gaggaacggc tgaaaaccta
     3301 tgcccacctg ttcgacgaca aagtgatgaa gcagctgaag cggcggagat acaccggctg
     3361 gggcaggctg agccggaagc tgatcaacgg catccgggac aagcagtccg gcaagacaat
     3421 cctggatttc ctgaagtccg acggcttcgc caacagaaac ttcatgcagc tgatccacga
     3481 cgacagcctg acctttaaag aggacatcca gaaagcccag gtgtccggcc agggcgatag
     3541 cctgcacgag cacattgcca atctggccgg cagccccgcc attaagaagg gcatcctgca
     3601 gacagtgaag gtggtggacg agctcgtgaa agtgatgggc cggcacaagc ccgagaacat
     3661 cgtgatcgaa atggccagag agaaccagac cacccagaag ggacagaaga acagccgcga
     3721 gagaatgaag cggatcgaag agggcatcaa agagctgggc agccagatcc tgaaagaaca
     3781 ccccgtggaa aacacccagc tgcagaacga gaagctgtac ctgtactacc tgcagaatgg
     3841 gcgggatatg tacgtggacc aggaactgga catcaaccgg ctgtccgact acgatgtgga
     3901 ccatatcgtg cctcagagct ttctgaagga cgactccatc gacaacaagg tgctgaccag
     3961 aagcgacaag aaccggggca agagcgacaa cgtgccctcc gaagaggtcg tgaagaagat
     4021 gaagaactac tggcggcagc tgctgaacgc caagctgatt acccagagaa agttcgacaa
     4081 tctgaccaag gccgagagag gcggcctgag cgaactggat aaggccggct tcatcaagag
     4141 acagctggtg gaaacccggc agatcacaaa gcacgtggca cagatcctgg actcccggat
     4201 gaacactaag tacgacgaga atgacaagct gatccgggaa gtgaaagtga tcaccctgaa
     4261 gtccaagctg gtgtccgatt tccggaagga tttccagttt tacaaagtgc gcgagatcaa
     4321 caactaccac cacgcccacg acgcctacct gaacgccgtc gtgggaaccg ccctgatcaa
     4381 aaagtaccct aagctggaaa gcgagttcgt gtacggcgac tacaaggtgt acgacgtgcg
     4441 gaagatgatc gccaagagcg agcaggaaat cggcaaggct accgccaagt acttcttcta
     4501 cagcaacatc atgaactttt tcaagaccga gattaccctg gccaacggcg agatccggaa
     4561 gcggcctctg atcgagacaa acggcgaaac cggggagatc gtgtgggata agggccggga
     4621 ttttgccacc gtgcggaaag tgctgagcat gccccaagtg aatatcgtga aaaagaccga
     4681 ggtgcagaca ggcggcttca gcaaagagtc tatcctgccc aagaggaaca gcgataagct
     4741 gatcgccaga aagaaggact gggaccctaa gaagtacggc ggcttcgaca gccccaccgt
     4801 ggcctattct gtgctggtgg tggccaaagt ggaaaagggc aagtccaaga aactgaagag
     4861 tgtgaaagag ctgctgggga tcaccatcat ggaaagaagc agcttcgaga agaatcccat
     4921 cgactttctg gaagccaagg gctacaaaga agtgaaaaag gacctgatca tcaagctgcc
     4981 taagtactcc ctgttcgagc tggaaaacgg ccggaagaga atgctggcct ctgccggcga
     5041 actgcagaag ggaaacgaac tggccctgcc ctccaaatat gtgaacttcc tgtacctggc
     5101 cagccactat gagaagctga agggctcccc cgaggataat gagcagaaac agctgtttgt
     5161 ggaacagcac aagcactacc tggacgagat catcgagcag atcagcgagt tctccaagag
     5221 agtgatcctg gccgacgcta atctggacaa agtgctgtcc gcctacaaca agcaccggga
     5281 taagcccatc agagagcagg ccgagaatat catccacctg tttaccctga ccaatctggg
     5341 agcccctgcc gccttcaagt actttgacac caccatcgac cggaagaggt acaccagcac
     5401 caaagaggtg ctggacgcca ccctgatcca ccagagcatc accggcctgt acgagacacg
     5461 gatcgacctg tctcagctgg gaggcgacaa gcgtcctgct gctactaaga aagctggtca
     5521 agctaagaaa aagaaagcta ggtacctgca tcctcaggtg caggctgcct atcagaaggt
     5581 ggtggctggt gtggccaatg ccctggctca caaataccac tgagatcttt ttccctctgc
     5641 caaaaattat ggggacatca tgaagcccct tgagcatctg acttctggct aataaaggaa
     5701 atttattttc attgcaatag tgtgttggaa ttttttgtgt ctctcactcg gaaggacata
     5761 tgggagggca aatcatttaa aacatcagaa tgagtatttg gtttagagtt tggcaacata
     5821 tgcccatatg ctggctgcca tgaacaaagg ttggctataa agaggtcatc agtatatgaa
     5881 acagccccct gctgtccatt ccttattcca tagaaaagcc ttgacttgag gttagatttt
     5941 ttttatattt tgttttgtgt tatttttttc tttaacatcc ctaaaatttt ccttacatgt
     6001 tttactagcc agatttttcc tcctctcctg actactccca gtcatagctg tccctcttct
     6061 cttatggaga tccctcgacg gatcggccgc aattcgtaat catgtcatag ctgtttcctg
     6121 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     6181 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     6241 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     6301 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     6361 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     6421 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     6481 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6541 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6601 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6661 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6721 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6781 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6841 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6901 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6961 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     7021 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7081 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7141 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7201 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7261 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7321 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7381 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7441 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7501 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7561 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7621 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7681 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7741 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7801 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7861 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7921 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7981 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     8041 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8101 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8161 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8221 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
     8281 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
     8341 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
     8401 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
     8461 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
     8521 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
     8581 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
     8641 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
     8701 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
     8761 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
     8821 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg agcgcgcgta
     8881 atacgactca ctatagggcg aattggagct ccaccgcggt ggcggccgct ctagagagct
     8941 tggcccattg catacgttgt atccatatca taatatgtac atttatattg gctcatgtcc
     9001 aacattaccg ccatgttgac attgattatt gactagttat taatagtaat caattacggg
     9061 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
     9121 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     9181 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     9241 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     9301 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     9361 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     9421 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     9481 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     9541 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     9601 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     9661 aagacaccgg gaccgatcca gcctccggtc gaccgatcct gagaacttca gggtgagttt
     9721 ggggaccctt gattgttctt tctttttcgc tattgtaaaa ttcatgttat atggaggggg
     9781 caaagttttc agggtgttgt ttagaatggg aagatgtccc ttgtatcacc atggaccctc
     9841 atgataattt tgtttctttc actttctact ctgttgacaa ccattgtctc ctcttatttt
     9901 cttttcattt tctgtaactt tttcgttaaa ctttagcttg catttgtaac gaatttttaa
     9961 attcactttt gtttatttgt cagattgtaa gtactttctc taatcacttt tttttcaagg
    10021 caatcagggt atattatatt gtacttcagc acagttttag agaacaattg ttataattaa
    10081 atgataaggt agaatatttc tgcatataaa ttctggctgg cgtggaaata ttcttattgg
    10141 tagaaacaac tacaccctgg tcatcatcct gcctttctct ttatggttac aatgatatac
    10201 actgtttgag atgaggataa aatactctga gtccaaaccg ggcccctctg ctaaccatgt
    10261 tcatgccttc ttctctttcc tacagctcct gggcaacgtg ctggttgttg tgctgtctca
    10321 tcattttggc aaagaattcc tcgagggccg gatccgaatt cctgtatttg tctgaaaaca
    10381 tgggccaggc tgttaccacc cccttaagtt tgactttaga ccactggaag gatgtcgaac
    10441 ggacagccca caacctgtcg gtagaggtta gaaaaaggcg ctgggttaca ttctgctctg
    10501 cagaatggcc aaccttcaac gtcggatggc cacgagacgg cacttttaac ccagacatta
    10561 ttacacaggt taagatcaag gtcttctcac ctggcccaca tggacatccg gatcaggtcc
    10621 cctacatcgt gacctgggaa gctatagcag tagacccccc tccctgggtc agacccttcg
    10681 tgcaccctaa acctcccctc tctcttcc
//
