LOCUS       sequence-235085-        4090 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Golden Gate (MoClo; CDS1) compatible luciferase gene from 
            Photinuspyralis with an intron for transient and stable expression 
            in plants .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4090)
  AUTHORS   Mortensen S, Bernal-Franco D, Cole LF, Sathitloetsakun S, Cram EJ, 
            Lee-Parsons CWT
  TITLE     EASI Transformation: An Efficient Transient Expression Method for 
            Analyzing Gene Function in Catharanthus roseus Seedlings.
  JOURNAL   Front Plant Sci. 2019 Jun 11;10:755. doi: 10.3389/fpls.2019.00755. 
            eCollection 2019.
  PUBMED    31263474
REFERENCE   2  (bases 1 to 4090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front Plant
            Sci."; date: "2019-06-11"; volume: "10:755. doi"; pages: " 
            10.3389/fpls.2019.00755. eCollection 2019"
COMMENT     Sequence Label: pSB63 - pL0_fLUC-I (CDS1)
FEATURES             Location/Qualifiers
     source          1..4090
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..42)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        110..214
                     /gene="bla"
                     /label=AmpR promoter
     CDS             434..1225
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      1396..1984
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1885..1904
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2138..2155
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(2299..2317)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          2377..2565
                     /label=IV2 intron
                     /note="modified second intron of the potato ST-LS1 gene 
                     (Vancanneyt et al., 1990)"
ORIGIN
        1 gtggctcttc agtggacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
       61 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      121 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      181 ataaatgctt caataatatt gaaaaaggaa gagtatgcgc tcacgcaact ggtccagaac
      241 cttgaccgaa cgcagcggtg gtaacggcgc agtggcggtt ttcatggctt gttatgactg
      301 tttttttggg gtacagtcta tgcctcgggc atccaagcag caagcgcgtt acgccgtggg
      361 tcgatgtttg atgttatgga gcagcaacga tgttacgcag cagggcagtc gccctaaaac
      421 aaagttaaac atcatgaggg aagcggtgat cgccgaagta tcgactcaac tatcagaggt
      481 agttggcgtc atcgagcgcc atctcgaacc gacgttgctg gccgtacatt tgtacggctc
      541 cgcagtggat ggcggcctga agccacacag cgatattgat ttgctggtta cggtgaccgt
      601 aaggcttgat gaaacaacgc ggcgagcttt gatcaacgac cttttggaaa cttcggcttc
      661 ccctggagag agcgagattc tccgcgctgt agaagtcacc attgttgtgc acgacgacat
      721 cattccgtgg cgttatccag ctaagcgcga actgcaattt ggagaatggc agcgcaatga
      781 cattcttgca ggtatcttcg agccagccac gatcgacatt gatctggcta tcttgctgac
      841 aaaagcaaga gaacatagcg ttgccttggt aggtccagcg gcggaggaac tctttgatcc
      901 ggttcctgaa caggatctat ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc
      961 gcccgactgg gctggcgatg agcgaaatgt agtgcttacg ttgtcccgca tttggtacag
     1021 cgcagtaacc ggcaaaatcg cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct
     1081 gccggcccag tatcagcccg tcatacttga agctagacag gcttatcttg gacaagaaga
     1141 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccattacg taaaaggcga
     1201 gatcaccaag gtagtcggca aataactgtc agaccaagtt tactcatata tactttagat
     1261 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     1321 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     1381 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     1441 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     1501 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     1561 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     1621 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     1681 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     1741 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     1801 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     1861 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     1921 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     1981 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     2041 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     2101 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     2161 ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatcact
     2221 ctgtggtctc aaatggagga cgccaaaaac ataaagaaag gcccggcgcc attctatcct
     2281 ctagaggatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     2341 cctggaacaa ttgcttttac agatgcacat atcgaggtaa gtttctgctt ctacctttga
     2401 tatatatata ataattatca ttaattagta gtaatataat atttcaaata tttttttcaa
     2461 aataaaagaa tgtagtatat agcaattgct tttctgtagt ttataagtgt gtatatttta
     2521 atttataact tttctaatat atgaccaaaa tttgttgatg tgcaggtgaa catcacgtac
     2581 gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat
     2641 acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg
     2701 ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa
     2761 ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg
     2821 caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat
     2881 tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct
     2941 cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca
     3001 ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga
     3061 actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg
     3121 gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc
     3181 ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga agagctgttt
     3241 ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac cctattttca
     3301 ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca cgaaattgct
     3361 tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg cttccatctt
     3421 ccagggatac gacaaggata tgggctcact gagactacat cagctattct gattacaccc
     3481 gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga agcgaaggtt
     3541 gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt atgtgtcaga
     3601 ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc cttgattgac
     3661 aaggatggat ggctacattc tggagacata gcttactggg acgaggacga acacttcttc
     3721 atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc ccccgctgaa
     3781 ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtctgccc
     3841 gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg
     3901 acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc
     3961 ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga
     4021 aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaagctttg
     4081 agaccacgaa
//
