LOCUS       sequence-235243-       12758 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression plasmid for Env from the BG505 HIV-1 isolate; 
            DS-SOSIP.6mut variant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12758)
  AUTHORS   Heredia JD, Park J, Choi H, Gill KS, Procko E
  TITLE     Conformational engineering of HIV-1 Env based on mutational 
            tolerance in the CD4 and PG16 bound states.
  JOURNAL   J Virol. 2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19.
  PUBMED    30894475
REFERENCE   2  (bases 1 to 12758)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Virol. 
            2019 Mar 20. pii: JVI.00219-19. doi: 10.1128/JVI.00219-19."
COMMENT     Sequence Label: pCEP4-BG505.DS-SOSIP.6mut-gp160
FEATURES             Location/Qualifiers
     source          1..12758
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    2603..2737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2627..2646)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2681..2700
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2880..2902)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      3462..5251
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(5557..7482)
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     primer_bind     complement(7917..7935)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        8003..8107
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8108..8176,8177..8968)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8326..8345)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9139..9727
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9628..9647
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        10158..10303
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             10346..11383
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     primer_bind     complement(11381..11400)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    11425..11473
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     enhancer        12127..12506
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12507..12710
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     12661..12681
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     12707..12731
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 gccaccatgc caatgggctc ccttcagccc ttggctaccc tgtaccttct tgggatgttg
       61 gtcgcatctg tgcttgccgc agaaaacttg tgggtaactg tctactatgg agtacccgtt
      121 tggaaagacg cagaaacaac gcttttttgc gcatccgacg cgaaggcata cgaaacggaa
      181 aagcacaatg tttgggcaac gcatgcctgc gtcccgacag acccaaatcc tcaggagatt
      241 caccttgaga atgtaacaga ggaattcaac atgtggaaga ataacatggt agaacaaatg
      301 catacggata ttatcagtct ttgggaccag agccttaaac cttgcgttaa acttacgccc
      361 ctctgcgtaa ctctgcaatg tactaacgta acgaataaca ttacggacga catgcgcggc
      421 gaaatgaaga attgcagctt taatatgaca acggagttgc gagataaaaa gcagaaggtg
      481 tacagcctct tctggaggct tgacgttgtg caaataaacg aaaaccaggg gaacaggagc
      541 aataactcca ataaagaata cagattgatc aactgtaaca ctagcgcatg tacccaggcc
      601 tgcccgaagg tgtcatttga gccgattcct atacactatt gcgccccggc agggtttgcg
      661 atcttgaaat gcaaggataa gaagtttaat ggaactggtc cctgtcccag tgtgagtacg
      721 gttcagtgta cccacggcat aaagccagtg gtatccactc agcttctcct caatggcagc
      781 ctcgcagagg aagaggtcat gatacggagt gagaatatta ctaacaatgc caagaatatc
      841 ttggtccaat ttaatacccc cgtccagatt aattgtaccc gacctatgaa tatgaccaga
      901 aaatccattc gcatcggacc aggtcaggcg ttctatgccc tgggagatat aataggtgac
      961 atccgacagg cgcattgcaa tgtaagcaag gccacatgga atgagactct gggtaaagtt
     1021 gtcaagcagc tcaggaagca tttcgggaac aataccatca tccgatttgc gaacagtagt
     1081 gggggtgatc ttgaagtaac tacacattca tttaactgcg gcggggagtt tttttattgt
     1141 aacacatccg ggttgttcaa ctccacttgg atatctaaca ctagtgttca gggatcaaac
     1201 tccacaggat caaacgatag tataacactt ccttgtcgca tgaaacagat tatcaacatg
     1261 tggcagcgaa tcggccagtg catgtacgcc ccgcctatcc agggagtaat acggtgcgta
     1321 agcaacataa cgggccttat acttacgcgc gatggaggca gcactaattc taccactgag
     1381 acgttccggc ctggcggtgg ggacatgcga gacaactggc ggtctgagtt gtacaagtac
     1441 aaagtggtta aaatcgaacc cctgggtgtc gccccaacga gatgtaaacg acgggtagtg
     1501 ggaaggagga gaaggcgacg ggcggtcggc attggggccg ttttcttggg ttttctgggg
     1561 gcggctggga gcactatggg agcagctagt atgaccctga ccgtacaggc gaggaacctc
     1621 ctgtccggta tagtgcaaca acagtcaaac cttcttaggg ctcctgaggc gcagcaacat
     1681 ctccttaagt tgactgtctg gggcataaag caactccaag cgcgggttct cgcggtggaa
     1741 agatacctta gggaccaaca acttttgggg atttggggtt gttcaggcaa actcatctgt
     1801 tgcactaacg ttccttggaa ctcctcatgg tctaaccgaa atctctccga gatctgggat
     1861 aatatgacat ggttgcagtg ggataaggag attagcaatt acactcaaat aatctatggg
     1921 ctcctggagg aaagccagaa tcagcaggag aagaatgaac aggatcttct ggcccttgat
     1981 aagtgggcaa gtctttggaa ttggttcgac atttcaaatt ggctttggta tataaagata
     2041 tttataatga ttgtcggggg tttgataggt ttgaggattg tgtttgctgt gctgtctgtt
     2101 atccacaggg ttaggcaggg gtatagtccg ctctcttttc aaacccatac ccccaacccc
     2161 aggggattgg accgacccga gcggattgag gaggaagacg gtgaacagga ccggggcaga
     2221 agtacccgcc ttgttagcgg gtttctggct ctggcgtggg acgacttgcg gagcctttgc
     2281 ctcttctgct accatcgcct cagagatttt atcttgatcg ctgccagaat agtagaattg
     2341 ttgggccaca gctccctcaa aggtttgaga cttggatggg aaggtcttaa atacctgtgg
     2401 aatttgctgg cttactgggg tagggagctg aagatttctg ccattaactt gttcgacaca
     2461 atagctattg cagttgcgga gtggactgac agagtcatag aaataggcca aagattgtgt
     2521 cgggcgtttc tccacattcc acggcggatt aggcaaggac ttgaacgggc cctcctttag
     2581 taactcgagg ccggcaaggc cggatccaga catgataaga tacattgatg agtttggaca
     2641 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     2701 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     2761 tatgtttcag gttcaggggg aggtgtggga ggttttttaa agcaagtaaa acctctacaa
     2821 atgtggtatg gctgattatg atccggctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     2881 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     2941 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgagg
     3001 tcgactctag aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc
     3061 cccttcttcg cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg
     3121 ggccctgttc cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag
     3181 ttgacatcct tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag
     3241 cagactttgc agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga
     3301 agctcttaca ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag
     3361 gctacaccaa cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg
     3421 gcattagcaa tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc
     3481 ccgggtagta gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg
     3541 ggaagcatat gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc
     3601 caccccatga gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa
     3661 ccattttagt cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa
     3721 tcttcgcctg cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa
     3781 cagtaaggtg tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa
     3841 tttggacggg gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc
     3901 ccttgggcaa taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa
     3961 tccatggggt ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta
     4021 tgggcaacac ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca
     4081 gggaccaaga caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg
     4141 gacgccgaca gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc
     4201 tccacgccaa tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg
     4261 agtgggggca cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg
     4321 tgtaataact tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa
     4381 aaccactaat ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg
     4441 cgcgattgct gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag
     4501 ggttgttggt cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg
     4561 gtagcatata ctacccaaat atctggatag catatgctat cctaatctat atctgggtag
     4621 cataggctat cctaatctat atctgggtag catatgctat cctaatctat atctgggtag
     4681 tatatgctat cctaatttat atctgggtag cataggctat cctaatctat atctgggtag
     4741 catatgctat cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag
     4801 catatgctat cctaatagag attagggtag tatatgctat cctaatttat atctgggtag
     4861 catatactac ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata
     4921 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     4981 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     5041 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     5101 tgctatccta atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata
     5161 tgatacccag tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg
     5221 gcgtgaattt tcgctgcttg tccttttcct gcatgctggt tgctcccatt cttaggtgaa
     5281 tttaaggagg ccaggctaaa gccgtcgcat gtctgattgc tcaccaggta aatgtcgcta
     5341 atgttttcca acgcgagaag gtgttgagcg cggagctgag tgacgtgaca acatgggtat
     5401 gcccaattgc cccatgttgg gaggacgaaa atggtgacaa gacagatggc cagaaataca
     5461 ccaacagcac gcatgatgtc tactggggat ttattcttta gtgcggggga atacacggct
     5521 tttaatacga ttgagggcgt ctcctaacaa gttacatcac tcctgccctt cctcaccctc
     5581 atctccatca cctccttcat ctccgtcatc tccgtcatca ccctccgcgg cagccccttc
     5641 caccataggt ggaaaccagg gaggcaaatc tactccatcg tcaaagctgc acacagtcac
     5701 cctgatattg caggtaggag cgggctttgt cataacaagg tccttaatcg catccttcaa
     5761 aacctcagca aatatatgag tttgtaaaaa gaccatgaaa taacagacaa tggactccct
     5821 tagcgggcca ggttgtgggc cgggtccagg ggccattcca aaggggagac gactcaatgg
     5881 tgtaagacga cattgtggaa tagcaagggc agttcctcgc cttaggttgt aaagggaggt
     5941 cttactacct ccatatacga acacaccggc gacccaagtt ccttcgtcgg tagtcctttc
     6001 tacgtgactc ctagccagga gagctcttaa accttctgca atgttctcaa atttcgggtt
     6061 ggaacctcct tgaccacgat gctttccaaa ccaccctcct tttttgcgcc tgcctccatc
     6121 accctgaccc cggggtccag tgcttgggcc ttctcctggg tcatctgcgg ggccctgctc
     6181 tatcgctccc gggggcacgt caggctcacc atctgggcca ccttcttggt ggtattcaaa
     6241 ataatcggct tcccctacag ggtggaaaaa tggccttcta cctggagggg gcctgcgcgg
     6301 tggagacccg gatgatgatg actgactact gggactcctg ggcctctttt ctccacgtcc
     6361 acgacctctc cccctggctc tttcacgact tccccccctg gctctttcac gtcctctacc
     6421 ccggcggcct ccactacctc ctcgaccccg gcctccacta cctcctcgac cccggcctcc
     6481 actgcctcct cgaccccggc ctccacctcc tgctcctgcc cctcctgctc ctgcccctcc
     6541 tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc ctgctcctgc
     6601 ccctcctgcc cctcctgctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     6661 ccctcctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg cccctcctgc
     6721 tcctgcccct cctgcccctc ctgctcctgc ccctcctgct cctgcccctc ctgctcctgc
     6781 ccctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg cccctcctgc
     6841 tcctgcccct cctgcccctc ctgcccctcc tgctcctgcc cctcctcctg ctcctgcccc
     6901 tcctgcccct cctgcccctc ctcctgctcc tgcccctcct gcccctcctc ctgctcctgc
     6961 ccctcctcct gctcctgccc ctcctgcccc tcctgcccct cctcctgctc ctgcccctcc
     7021 tgcccctcct cctgctcctg cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc
     7081 tcctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg cccctcctgc
     7141 ccctcctcct gctcctgccc ctcctcctgc tcctgcccct cctgctcctg cccctcccgc
     7201 tcctgctcct gctcctgttc caccgtgggt ccctttgcag ccaatgcaac ttggacgttt
     7261 ttggggtctc cggacaccat ctctatgtct tggccctgat cctgagccgc ccggggctcc
     7321 tggtcttccg cctcctcgtc ctcgtcctct tccccgtcct cgtccatggt tatcaccccc
     7381 tcttctttga ggtccactgc cgccggagcc ttctggtcca gatgtgtctc ccttctctcc
     7441 taggccattt ccaggtcctg tacctggccc ctcgtcagac atgattcaca ctaaaagaga
     7501 tcaatagaca tctttattag acgacgctca gtgaatacag ggagtgcaga ctcctgcccc
     7561 ctccaacagc ccccccaccc tcatcccctt catggtcgct gtcagacaga tccaggtctg
     7621 aaaattcccc atcctccgaa ccatcctcgt cctcatcacc aattactcgc agcccggaaa
     7681 actcccgctg aacatcctca agatttgcgt cctgagcctc aagccaggcc tcaaattcct
     7741 cgtccccctt tttgctggac ggtagggatg gggattctcg ggacccctcc tcttcctctt
     7801 caaggtcacc agacagagat gctactgggg caacggaaga aaagctgggt gcggcctgtg
     7861 aggatcagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct
     7921 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     7981 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     8041 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     8101 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     8161 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     8221 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     8281 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     8341 attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     8401 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     8461 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     8521 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     8581 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     8641 cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     8701 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     8761 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     8821 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     8881 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     8941 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     9001 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     9061 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     9121 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     9181 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     9241 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     9301 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     9361 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     9421 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     9481 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     9541 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     9601 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     9661 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     9721 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttgaag
     9781 ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc
     9841 gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa
     9901 cgccagcaag acgtagccca gcgcgtcggc cccgagatgc gccgcgtgcg gctgctggag
     9961 atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc
    10021 aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccctgct
    10081 tcatccccgt ggcccgttgc tcgcgtttgc tggcggtgtc cccggaagaa atatatttgc
    10141 atgtctttag ttctatgatg acacaaaccc cgcccagcgt cttgtcattg gcgaattcga
    10201 acacgcagat gcagtcgggg cggcgcggtc cgaggtccac ttcgcatatt aaggtgacgc
    10261 gtgtggcctc gaacaccgag cgaccctgca gcgacccgct taacagcgtc aacagcgtgc
    10321 cgcagatccc ggggggcaat gagatatgaa aaagcctgaa ctcaccgcga cgtctgtcga
    10381 gaagtttctg atcgaaaagt tcgacagcgt ctccgacctg atgcagctct cggagggcga
    10441 agaatctcgt gctttcagct tcgatgtagg agggcgtgga tatgtcctgc gggtaaatag
    10501 ctgcgccgat ggtttctaca aagatcgtta tgtttatcgg cactttgcat cggccgcgct
    10561 cccgattccg gaagtgcttg acattgggga attcagcgag agcctgacct attgcatctc
    10621 ccgccgtgca cagggtgtca cgttgcaaga cctgcctgaa accgaactgc ccgctgttct
    10681 gcagccggtc gcggaggcca tggatgcgat cgctgcggcc gatcttagcc agacgagcgg
    10741 gttcggccca ttcggaccgc aaggaatcgg tcaatacact acatggcgtg atttcatatg
    10801 cgcgattgct gatccccatg tgtatcactg gcaaactgtg atggacgaca ccgtcagtgc
    10861 gtccgtcgcg caggctctcg atgagctgat gctttgggcc gaggactgcc ccgaagtccg
    10921 gcacctcgtg cacgcggatt tcggctccaa caatgtcctg acggacaatg gccgcataac
    10981 agcggtcatt gactggagcg aggcgatgtt cggggattcc caatacgagg tcgccaacat
    11041 cttcttctgg aggccgtggt tggcttgtat ggagcagcag acgcgctact tcgagcggag
    11101 gcatccggag cttgcaggat cgccgcggct ccgggcgtat atgctccgca ttggtcttga
    11161 ccaactctat cagagcttgg ttgacggcaa tttcgatgat gcagcttggg cgcagggtcg
    11221 atgcgacgca atcgtccgat ccggagccgg gactgtcggg cgtacacaaa tcgcccgcag
    11281 aagcgcggcc gtctggaccg atggctgtgt agaagtactc gccgatagtg gaaaccgacg
    11341 ccccagcact cgtccggatc gggagatggg ggaggctaac tgaaacacgg aaggagacaa
    11401 taccggaagg aacccgcgct atgacggcaa taaaaagaca gaataaaacg cacgggtgtt
    11461 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca
    11521 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca
    11581 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc
    11641 actggccccg tgggttaggg acggggtccc ccatggggaa tggtttatgg ttcgtggggg
    11701 ttattatttt gggcgttgcg tggggtcagg tccacgactg gactgagcag acagacccat
    11761 ggtttttgga tggcctgggc atggaccgca tgtactggcg cgacacgaac accgggcgtc
    11821 tgtggctgcc aaacaccccc gacccccaaa aaccaccgcg cggatttctg gcgtgccaag
    11881 ctagtcgacc aattctcatg tttgacagct tatcatcgca gatccgggca acgttgttgc
    11941 cattgctgca ggcgcagaac tggtaggtat ggaagatcta tacattgaat caatattggc
    12001 aattagccat attagtcatt ggttatatag cataaatcaa tattggctat tggccattgc
    12061 atacgttgta tctatatcat aatatgtaca tttatattgg ctcatgtcca atatgaccgc
    12121 catgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc
    12181 atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac
    12241 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa
    12301 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag
    12361 tacatcaagt gtatcatatg ccaagtccgc cccctattga cgtcaatgac ggtaaatggc
    12421 ccgcctggca ttatgcccag tacatgacct tacgggactt tcctacttgg cagtacatct
    12481 acgtattagt catcgctatt accatggtga tgcggttttg gcagtacacc aatgggcgtg
    12541 gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt
    12601 tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taataacccc gccccgttga
    12661 cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga
    12721 accgtcagat ctctagaagc tgggtaccag ctgctagc
//
