LOCUS       sequence-235342-       15700 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  miniMos vector expresses ERKKTR(S43A,T55A,T62A)-mClover and 
            mCherry-H2B in C. elegans VPCs; use as control for pCC324 (Contains 
            Neomycin resistance).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15700)
  AUTHORS   de la Cova C, Townley R, Regot S, Greenwald I
  TITLE     A Real-Time Biosensor for ERK Activity Reveals Signaling Dynamics 
            during C. elegans Cell Fate Specification.
  JOURNAL   Dev Cell. 2017 Sep 11;42(5):542-553.e4. doi: 
            10.1016/j.devcel.2017.07.014. Epub 2017 Aug 17.
  PUBMED    28826819
REFERENCE   2  (bases 1 to 15700)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2017.07.014"; journalName: "Dev Cell"; date: 
            "2017-09-11- 11"; volume: "42"; issue: "5"; pages: "542-553.e4"
COMMENT     Sequence Label: pCC335
FEATURES             Location/Qualifiers
     source          1..15700
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     283..299
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     283..299
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7875..7896)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     8483..8504
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8514..8567
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     primer_bind     complement(10699..10716)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10699..10715)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             11537..12331
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11591..11610)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     12201..12220
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     13032..13048
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     13033..13049
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     13704..13726
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(13764..13782)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        13850..13954
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(13955..14023,14024..14815)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGSDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14173..14192)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      14986..15574
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15475..15494
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcaccgtacg tctcgaggaa ttctatcagg tgtacaagta tgaaatgtcg tttcgaacat
       61 atagatgtct cgcaaacgta aatatttatc gattgtcata aaactttgac cttgtgaagt
      121 gtcaaccttg actgtcgaac caccatagtt tggcgcgaat tgagcgtcat aattgtttac
      181 tctcagtgca gtcaacatgt cgagtttcgt gccgaataaa gagcaaacgc ggacagtatt
      241 aattttctgt tttcatttga agaaaacagc tgcggccgga tccaggaaac agctatgacc
      301 atgattacgc caagctacgt aatacgactc actagcagag cgaattctga tctacaaata
      361 tattatggat cggtttccgt tttataggaa aaacacgcag aggtggcaaa actccctgcg
      421 acacaacttg tcattcaacg actgcttcat caagatccca agaagagccg atcgtcctgg
      481 gaagggctcc tactgggcgg tgcacccaaa tgcctccgga atgttcgaga acggcagctg
      541 cttacggcgt cggaaacgat ttcgggtaag tcttcggtta ctgtacttat caaattgtgt
      601 aaattaatga tcttatcact tcaaagagaa acgcaaaaat ctccaacaaa aaaaaacgaa
      661 aaattgattt agagagggcg cgcgcgtgct tcccccttca cgattttgag agagaaagac
      721 aattctacag taatcctcaa ggttggtcga tgacagaaaa ggcgagaaat ttgttttaaa
      781 tacgattgag agactattgg gggaagagat tatgatgaac ggctgcagga ttggataaga
      841 ctttgcagat tttgatgtgg ggtactgtag tttaaaggga agactgtgaa caaatattta
      901 agatagtgtg gctatgcgca ggcttaggct taaatttagg tttaggctta ggcttaggct
      961 tagtcttagg ctcagtctca gggttaggtt taggcttagg ctcaggttca gacttaggtt
     1021 caggcttagc ctattgctta ggcttaggct taggcttagg cttaggctcc ggcttaggat
     1081 taggcttagg cttaggttca ggctcagggt taggcttagg gtaaagctta ggttctggct
     1141 taggcttaga cttatgctta agattaggca atgaagagta cctgtgtatt tggttcagcc
     1201 gaaattgcgc aactttgaga atgtacaaaa ttcttaaaaa attgcagagt tggaactttc
     1261 agaaatcgca aatgccttcg gagaatagga agcttcggca ctgtggtttt ggtactgacc
     1321 tgcaattttc aaatcattct gcaaagtttt aaatttgaca aaaatttttt atcaaaatgg
     1381 ttttgaaacg tttatctaca tagggtaatt aacctcgccc cccccccnnc cncccaaaat
     1441 attttcaatt ttcactcaat tttactcaat acaattaaac agtcatatta agatgaattg
     1501 acttacgcgc gcgaaatact ctcctcccgg tactacccaa taaaatgtgc catctgttgc
     1561 aatagttgta ctcctactac ttctacgggg gggggcaaca gatgcctcct atccaatatt
     1621 gtagtgttat gttgaaatga aaatgagtga cttgtgtctg tgtgtgtatg atgaagtagt
     1681 tagaaaagag gacaaagaaa aagtaataag aagttgcaag agtttgtttg agtggggtga
     1741 gcggggtttg cacataactt taaatttttt tcaactattc ggcttgggaa ctctacacct
     1801 attgaaatat aaggctgtag gcaagcactt aggcatgtag gtaggaagaa cggggacatg
     1861 actatgcaaa caggtataat tctgttccaa tgtactgttc caaatatcaa atcctccata
     1921 cagtctgtcc tccaaccaca agcccattaa aatgacagtc aggctgtcca gcggggggtc
     1981 ccccgccgaa aaagaagggc tgtcgcgggg cgctagctaa tatgtgacac atatacatat
     2041 aactgtatca cttgtacatg tacataagac ggattggaaa caaaagtgat tctctttgcc
     2101 tatggttcat tccatttcgc gcaatgtttt aggtagtacg gtagattaaa tggccttggg
     2161 gcgaggaggg gaggactaat cacttggagg ggaaagaaga ggcaagacaa agattgatgc
     2221 ggtttgaatt tagatattta ggtggtggag cgaggtgtca atgtttgtca ggtgcctgat
     2281 ttattagtat atgtacttta agagatgaat tatagaagtg ctgcaaattt gaaaggcata
     2341 actgtaaggt accttttaaa ttactaccgg catgtggtgt caaagtgttt cagttcggtt
     2401 tgatctacaa agaatgctgg agaagagacg cagacttttt cggggcggtg ctcgggaaga
     2461 gctgatttac gtcgatttac agttttcctc gtttctagaa tttcgtaaat cgacacaaat
     2521 cggctttttc cgcgccccgt cctttgggtc ccgggatgtc tctgtctctt caactatcta
     2581 cactatctgt actggtaata gtgcattttc taaaattgaa tcataaaatt ttgctgtaaa
     2641 agttgaacgt tttttaaaca cagttttaaa aaatatttaa aaaattcaat ttcaggctcg
     2701 cggcggccaa gacgacgacg acgacgactt ccaccacccg gctccatcga aaatctctcg
     2761 caaaaatcct cttccactgc tcccggagcc cccaataacc ccaccactcc tctcctccct
     2821 tttcccaaat cttccaccat cactcccaaa tttctgcctg tttcctccag gcatggatcg
     2881 atcgtagagc actagtggat ccagagcgca tgctctagtt ttgtaaatct acaatgttca
     2941 aacgctggac ttcatgctaa gatcctgaaa ataggcagta ggtaggtagg ttcagccata
     3001 aataccaaaa aacatgcctg acaggcctac ctattcagga ctacctacgc ctgtctcgtg
     3061 cctatatgtc tattctacta ttttagtaaa catacactct tcaaaaacgc aatatcaccg
     3121 aaaatagcaa aaagtaagcg gaaagcatgc atgtaggcaa gtaggcagat aggaataggt
     3181 catgtataaa aacatacctg taactacatg ccttgtctat ttctcaaagn cccccccccc
     3241 ccattgttga cgaaaattga caccgacaat tgccctattc tttctcctta tttctctcct
     3301 ctccattcta tttcataact tttcatcagt atattcactc aattggaaga aaatatgtat
     3361 gtctacaaca acctacaaga agacgtcaat ttcatttcgg accccccttc tccacgtttg
     3421 tcgagaaagt cgtaaagagc atatggagga gcaacaaggg ggcttagttt cacattttaa
     3481 ttgttgcgcc ttcttgttga cctcctggac aggaaaatgg atgagtttat gatgttggga
     3541 gaaacaaatt tttggaattt ttaatgaaaa cctatttgga aagtcctgaa gacctaacgt
     3601 ttgaattttt tgagaaaaaa atgtacaaaa aatacagttt attctagtgg cttgaaaggc
     3661 aaattcaaac tcccgccaaa gaaaggcacc gtccaatcat cgacgccggc cacacccact
     3721 gctcattgct gattcgtcga tagggagttt ctgtagaaga atataaaggt actgtaggca
     3781 actgtagcgg tactgtagaa gagtgtggat gtactgttgg tgtaatgtag aggtactata
     3841 gatattctct aggggtactg tatgaaaatg tatggattct gtggaaatat tgtaggaaac
     3901 tgtgggggta ctgaagatat actgtagatg ttctgtgtgg tttttaaggg gtactgtagg
     3961 aatactgaag agcgtttttt gcaatagaat ccgctcatat cttgactatc ccagcattgt
     4021 cggatttgtt tttcaaaaat cattttctta aagctatttt ctctggaaaa aactcttccc
     4081 aattaccgta ccagtgtcat ttttccttac tgaaaacaaa ggaaaacaag taggttgagg
     4141 aaagaaaaac aattgttttt aatcttgaat gagtaagctt ggcacacaca tgtctctaaa
     4201 acaaattaac tacggagtac ggtagtcttg tttttcaaaa gagaaccaac tgggtacata
     4261 ttagttttca ttttactccc cctccttgca ttttcctcgt tttcataaca aatcaatttg
     4321 gagtcatatt catggaaatt gaaaaagaca agaagacaag gagtaggaga aggtaatgag
     4381 aaaaatgggt ttaaggtttt gaaaagaaat tgacaatcac ataaaaccta gaggggtgct
     4441 aagtaagcaa tgtgatcgga aattgtaatg gattagggtg aatggagggt gtttggtttt
     4501 gggtttgatt gaatttggcg ctagttgcca agagttgccg ggaatctggc ttttgctggg
     4561 tggtaaaatt catagaaaaa tttgtagaaa aatcggcggc cgagtttttc cttttttgcg
     4621 accacgcagg ttgcaaaaag cataaattct ggttcatgtt cacaaaacct cgcgcaagtt
     4681 tttaaaatct tgagtagggg cctgcctgag gtgctgaggt gaattagacc aagtttttgt
     4741 tcgcaactac caaaaacttc agaaaaattt ttgttaatct catcataaaa ttcgtttact
     4801 tcggctaatt tttattagtt ataagtctat aaaaaacctc caaaatatcc cctttttaag
     4861 gttaaatttt tgaaaatttt cgaattataa taaagtctga aaattgtcaa aatttcctgc
     4921 aattgaccgt gttggcaatt ttggcaattt cagcaagagc cgaaattacc aattgcacgg
     4981 tctcgtcaag gttgtcagtg tctagataaa cattatgaaa atttcgaaaa gctgacgaaa
     5041 actcttcaga gtatatgact caaacttttg atgattttga tgcgacattg ctcaaagtta
     5101 cataaaaatc taattttaaa atgttataaa gcttaatccc tttctaattc ctaataagta
     5161 ccgaaagtac agtagttcta gtcaccttgc caaaacaaaa ggataaacag gaaaactaaa
     5221 aagtttctaa aaagaaaagc cgcgaaaagg agagagagag atgggataat gttacaaatt
     5281 ggacctgtta agtgtcggtt taatgaaccg agcttctaaa tatggcacga ggagcggtgg
     5341 tgtggccagc ggcgccgcac cgtctgcagt caaccgtgcg atcgagcggc acctttgatc
     5401 caaaaaatgc cgcgattttt gccgcgctct ccccaggcag gcagtgggga agagatgtcc
     5461 tttatgaagg agattgacgg gaggaggagg aggaggaaac cgtttttgat taactggtgg
     5521 atggcattgc ggtttttatt cttccgtaag gacaactggt tcttttttct tctattttga
     5581 tgtcttcgtt ttgtctttac tggtctgtct tcgaagttgc tgagctgatt tagaacattg
     5641 taaatttgta agaaaattga tttttatcaa cataattacg aagtatcacg ttttacgtgt
     5701 gtttttagag ttttcaagta aaaaatttga aatcagcggt attcaaaacc taaacctttt
     5761 cacggggact cgatcccttg accttttgct ccaaaggcat tgctcttacc cactgagcca
     5821 tccgtggcgc cgttttctta gggcgccgta ccaagtggcg cagtggctaa gagggacgac
     5881 tttgaagcaa acggtcatga gttcgagccc ccgggagttg atttaaattt tctgattacg
     5941 actcgctgcg ccctaaaggt ccctttagct ccccattgat catttatccc aaatctcatt
     6001 tataaaatct ttgtcccgag acaaacccag tcatatttaa ttagaataat cactaccctt
     6061 ttttgattct cccacataca atttttttaa accgagagta cggtaggtaa taacaattta
     6121 tggagaagaa tattaggtga tgagaatggg tgtctctact actattatat atattacttt
     6181 attcattcat taaattatgg tggtggtggt gacgatgaga aactttacgg aatttatgag
     6241 tgaaattgtt ccaaaaaagt ggtgaagtgt caaatggaat atctaactgg gaggggggtg
     6301 aataaggaca atggggttag tgtactctcg agaatatgga attagaaaat gaggaaaact
     6361 tgcaaaaaat ggagctttga aaaaaactag ctgaaaacat cctttcagag ttggatttta
     6421 agattttaag atttctctaa atcattgtag tgaaattttc tactgaggtt ttctagtttc
     6481 tttttcattt ggtatacatt tttttgtaga aaatgttcac caaaactttt ttcgaatgca
     6541 taataattca acattttcta tgctttttaa attatttttc ttgttgcaga aaacccaaac
     6601 taccgtactt tttcactagg tggtctccaa actttaatcc gttataactc cacaaatatt
     6661 tttgatataa aaaagttgtc aattaacaaa atgtgcaaaa ttttatgacc aatatttaat
     6721 cagttgacag ttttttgata tctcaggtaa ccgcagagtt atgagcgttt gaagttgaag
     6781 cgtgctctag gctctccagg tgacccaaat atttgaatat tttagtaatt ctcatgattt
     6841 taaaattgtc caacaaaaat tcaaatattg acactaattc ttcccaccta gacaccccac
     6901 cattccatgg actaatcccg ttaattcgaa cgcttccccc ctaccgctct cggtgtcact
     6961 caaaatttca aacaagtcgt gacagttctc gggtggtctg ataccagttt acctacattt
     7021 tcaaaaaaaa acacaatcaa ctctttcatc ttcagtattc cagtgaatcc cgtcccacac
     7081 cagatgcccc aactcagcgg gggctgcgtg atacccggtc agcagcagca ccagcaacag
     7141 gagtaactgg ttcctcccaa atataggtta gtgatgtgaa tgtaaaacaa agacttattt
     7201 ttctattaga aatgtgttgt cattttcctt attacaagtt gttgcctttt tcccttacgg
     7261 gcggtctcgg taagcgctgt ctccccgttg gccagccccg cccactcaac gctccgccct
     7321 tcttctcctt ctaaggcttg gcttctcaaa caaattgact tttcccaatc gctaagattt
     7381 caatatattt tgatatattc caaaaccttc tttattaatt atcaatccaa gcaaaactct
     7441 atacatattc cctgaaaaag atccccggga ttggccaaag gacccaaagg tatgtttcga
     7501 atgatactaa cataacatag aacattttca ggaggaccct tggctagcgt cgacgccaga
     7561 tctgcggcgg ccgcaccatg aagggccgaa agcctcggga cctggaactt ccacttagcc
     7621 caagcctgct gggtggccag ggacctgaac ggactccagg atcaggaaca agctctggtc
     7681 ttcaggcacc ggggccagcg cttgcgccca gcaagcgtag cggtctcgag gacccagcgg
     7741 cgccatcgaa gaagccaaaa gccccatcgg tgtcgtcaag gctggagcgc ctgaccctcc
     7801 agtccagctt tcaattccca tccactagta tggtgagcaa gggcgaggag ctgttcaccg
     7861 gggttgtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtcc
     7921 gcggcgaggg cgagggcgat gccaccaacg gcaagctgac cctgaagttc atctgcacca
     7981 ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cttcggctac ggcgtggcct
     8041 gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg
     8101 aaggctacgt ccaggagcgc accatctctt tcaaggacga cggtacctac aagacccgcg
     8161 ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact
     8221 tcaaggagga cggcaacatc ctggggcaca agctggagta caacttcaac agccacaacg
     8281 tctatatcac ggccgacaag cagaagaacg gcatcaaggc taacttcaag atccgccaca
     8341 acgttgagga cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg
     8401 acggccccgt gctgctgccc gacaaccact acctgagcca tcagtccgcc ctgagcaaag
     8461 accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgagggca
     8521 gaggaagtct gctaacatgc ggtgacgtcg aggagaatcc tggcccagga ggtagggccg
     8581 gctctgtctc aaagggtgaa gaagataaca tggcaattat taaagagttt atgcgtttca
     8641 aggtgcatat ggagggatct gtcaatgggc atgagtttga aattgaaggt gaaggagaag
     8701 gccgaccata tgagggaaca caaaccgcaa aactaaaggt aagtttaaac atatatatac
     8761 taactaaccc tgattattta aattttcagg taactaaagg cggaccatta ccattcgcct
     8821 gggacatcct ctctccacag ttcatgtatg gaagtaaagc ttatgttaaa catccggcag
     8881 atataccaga ttatttgaaa ctttcattcc cggagggttt taagtgggaa cgcgtaatga
     8941 attttgaaga cggaggagtt gttacagtga cgcaagactc aaggtaagtt taaacagttc
     9001 ggtactaact aaccatacat atttaaattt tcagcctcca agatggagaa tttatttata
     9061 aagtcaaact tcgaggaacg aatttcccct cggatggacc tgttatgcag aagaagacta
     9121 tgggatggga agcttcaagt gaaagaatgt accctgaaga cggtgctctt aagggagaga
     9181 ttaaacaacg tcttaaattg aaagatggag gacattacga tgctgaggta agtttaaaca
     9241 tgattttact aactaactaa tctgatttaa attttcaggt gaagacaact tacaaagcca
     9301 aaaaaccagt tcagctgcca ggagcgtaca atgttaatat taaactggat atcacctccc
     9361 acaacgagga ttacactatc gttgagcaat atgaaagagc tgaagggcgg cactcgacag
     9421 gtggcatgga tgaattgtat aagggaggag gaggaggtgg aatgccacca aagccatctg
     9481 ccaagggagc caagaaggcc gccaagaccg ttacgaagcc aaaggacgga aagaagagac
     9541 gtcatgcccg taaggaatca tactccgtct acatctaccg tgtcctcaag caagttcatc
     9601 cagacactgg agtttcctcc aaagccatgt ctatcatgaa ctcttttgtc aacgatgtct
     9661 tcgagcgtat tgctgctgaa gcatcccgtc ttgctcacta caacaagcgt tccacaatct
     9721 catcccgcga aattcagacc gctgtccgtc tgatccttcc aggagagctt gccaagcacg
     9781 ccgtgtctga gggaaccaag gccgttacca agtacacttc cagcaagtaa gcggccgcca
     9841 gctccgcatc ggccgctgtc atcagatcgc catctcgcgc ccgtgcctct gacttctaag
     9901 tccaattact cttcaacatc cctacatgct ctttctccct gtgctcccac cccctatttt
     9961 tgttattatc aaaaaacttc tcttaatttc tttgtttttt agcttctttt aagtcacctc
    10021 taacaatgaa attgtgtaga ttcaaaaata gaattaattc gtaataaaaa gtcgaaaaaa
    10081 attgtgctcc ctccccccat taataataat tctatcccaa aatctacaca atgttctgtg
    10141 tacacttctt atgtttttta cttctgataa atttttttga aacatcatag aaaaaaccgc
    10201 acacaaaata ccttatcata tgttacgttt cagtttatga ccgcaatttt tatttcttcg
    10261 cacgtctggg cctctcatga cgtcaaatca tgctcatcgt gaaaaagttt tggagtattt
    10321 ttggaatttt tcaatcaagt gaaagtttat gaaattaatt ttcctgcttt tgctttttgg
    10381 ggtttcccct attgtttgtc aagatttcga ggacggcgtt tttcttgcta aaatcacaag
    10441 tattgatgag cacgatgcaa gaaagatcgg aagaaggttt gggtttgagg ctcagtggaa
    10501 ggtgagtaga agttgataat ttgaaagtgg agtagtgtct atggggttct tcgaatgcat
    10561 cgcgcgcacc gtacgtctcg aggaattcct gcaggatatc tggatccacg aagcttccca
    10621 tggtgacgtc accggttcta gatacctagg tgagctctgg taccctctag tcaaggcctt
    10681 aagtgagtcg tattacggac tggccgtcgt tttacaggcc ggatgggcca tggcacctta
    10741 acatttctaa attttagttt aataaaaaaa ttttaaaata atacgagttt tgagactaac
    10801 tttcaataaa aaccaaaaaa ttgcttcaaa aaagagctcg cgccgattta cgataaacac
    10861 agcgcgctac gacactccga aaaccgcgcc gcaccattac attctccgcc ccaccgcacg
    10921 gtttgcggaa ggcgatgaaa ttgctgggcc taaaatttta accctttttt tcccgttttt
    10981 caactgtttt cccgattttt tcgatatttt tttcaggttt ttagttagaa aagtatataa
    11041 aaattgcatt atttgtagtt ttcgctcgta atttgtgata gaaaacggcc aatttgccta
    11101 gatttgtgat tctaccaagt ggaatttttg ggaaaatatc aatattccac gatttttatt
    11161 caaatcataa cgtttcgaaa ttatcagctt aaataaaact aaaataaata ataaaatact
    11221 aaaaaccctg aatttctcgg ttctcggttc agcttgagtt cgttctatac acgttatcga
    11281 tttttgtcat ttcgtttaat catgggtttt gattcatttt aaatatgaaa tttgatttat
    11341 tcctcgtttc gaagttggtt tgtcttgtcc ggccggtaaa caaatgttat taaaaaaaat
    11401 aataaatttc gaaatttttc gtaatgttcc gcaatcgtgt ttttccgttt atctcgtctt
    11461 cccttccttc aaatatacta tttattttct caattcacat attttcagcc agccctgctc
    11521 aacaagtgga ataaaaatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
    11581 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
    11641 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
    11701 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
    11761 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
    11821 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
    11881 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
    11941 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
    12001 ggatgatctg gacgaagagc atcaggggct cgcaccagcc gaactgttcg ccaggctcaa
    12061 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
    12121 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
    12181 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
    12241 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
    12301 cttctatcgc cttcttgacg agttcttctg agtccaatta ctcttcaaca tccctacatg
    12361 ctctttctcc ctgtgctccc accccctatt tttgttatta tcaaaaaact tctcttaatt
    12421 tctttgtttt ttagcttctt ttaagccacc tctaacaatg aaattgtgta gattcaaaaa
    12481 tagaattaat tcgtaataaa aagtcgaaaa aaattgtgct ccctcccccc attaataata
    12541 attctatccc aaaatctaca caatgttctg tgtacacttc ttatgttttt tacttctgat
    12601 aaattttttt gaaacatcat agaaaaaacc gcacacaaaa taccttatca tatgttacgt
    12661 ttcagtttat gaccgcaatt tttatttctt cgcacgtctg ggcctctcat gacgtcaaat
    12721 catgctcatc gtgaaaaagt tttggagtat ttttggaatt tttcaatcaa gtgaaagttt
    12781 atgaaattaa ttttcctgct tttgcttttt ggggtttccc ctattgtttg tcaagatttc
    12841 gaggacggcg tttttcttgc taaaatcaca agtattgatg agcacgatgc aagaaagatc
    12901 ggaagaaggt ttgggtttga ggctcagtgg aaggtgagta gaagttgata atttgaaagt
    12961 ggagtagtgt ctatggggtt tttgccttaa atgacagaat acattcccaa tataccaaac
    13021 ataactgttt ctcgaggtcg acggtatcga taagcttgat ccggatctgc ggcttactca
    13081 ccagacctgg ccccatccga ttaccaccta ttcgcttcga tgggacacgc actcgctgag
    13141 cagcgcttcg attcttacga aagtgtgaaa aaatggctcg atgaatggtt cgccgcaaaa
    13201 gacgatgagt tctactggcg tggaatccac aaattgcccg agagatggga aaaatgtgta
    13261 gctagcgacg gcaaatactt tgaataaatg attttttctt tttccacaaa atttaacgtg
    13321 ttttttgatt taaaaaaaac gacatttcat acttgtacac ctgatatcta gatacctagg
    13381 tgagctctgg tacccccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    13441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    13501 cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    13561 acaccgcata tggtgcactc tcagtgcaat ctgctctgat gccgcatagt taagccagcc
    13621 ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc
    13681 ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc
    13741 accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
    13801 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    13861 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
    13921 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
    13981 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
    14041 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
    14101 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
    14161 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
    14221 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
    14281 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
    14341 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
    14401 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
    14461 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
    14521 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
    14581 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
    14641 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggtc
    14701 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
    14761 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
    14821 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
    14881 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
    14941 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    15001 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    15061 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    15121 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    15181 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    15241 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    15301 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    15361 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    15421 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    15481 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    15541 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    15601 gttcctggtc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    15661 tgtggataac cgtattaccg cctttgagtg agctgatacc
//
