LOCUS       sequence-23537-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Takafuji V, Forgues M, Unsworth E, Goldsmith P, Wang XW
  TITLE     An osteopontin fragment is essential for tumor cell invasion in 
            hepatocellular carcinoma.
  JOURNAL   Oncogene. 2007 Sep 27. 26(44):6361-71.
  PUBMED    17452979
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Oncogene. 
            2007 Sep 27. 26(44):6361-71."
COMMENT     Sequence Label: pDest490-OPN-10 kDa
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(202..226)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             246..269
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     508..528
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(593..610)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    599..710
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cagtttgtca aaaagttggc accatgagaa ttacagtgat ttgcttttgc ctcctaggca
       61 tcacctgtgc cctgaggtca aaatctaaga agtttcgcag acctgacatc cagtaccctg
      121 atgctacaga cgaggacatc acctcacaca tggaaagcga ggagttgaat ggtgcataca
      181 aggccatccc cgttgcccag gacccaactt tcttgtacaa agtggttcga tctagaatgg
      241 ctagcgacta caaggacgac gatgacaagt agtaatcgag tacaactata actcacacaa
      301 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
      361 caacattgaa gatggatccg ttcaactagc agaccattat caacaaaata ctccaattgg
      421 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg ccctttcgaa
      481 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
      541 tacacatggc atggatgagc tctacaaata atgaattaaa cccgctgatc agcctcgact
      601 gtgccttcta gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg
      661 gaaggtgcca ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg
      721 agtaggtgtt ngctttnnng gggggggggg gggggncagg acancagggg gaggatggga
      781 agacatacag gctgntggga ngcgtggnnt tgnntctgag cgaaaacagt gggtnagggn
      841 tccccccctg a
//
