LOCUS       sequence-235386-       13186 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Localization: Actin/Focal Adhesions, Excitation: 505 / 569, 
            Emission: 516 / 581.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13186)
  TITLE     Davidson Photoactivatable Fluorescent Proteins
REFERENCE   2  (bases 1 to 13186)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: tdEos-FilaminA-C-14
FEATURES             Location/Qualifiers
     source          1..13186
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        91..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        395..598
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     549..569
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             643..1320
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="MSAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVK
                     EGGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIAR
                     NDITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLL
                     EGNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPD
                     NARR"
     CDS             1369..2043
                     /codon_start=1
                     /product="green-to-red photoswitchable fluorescent protein 
                     (Wiedenmann et al., 2004)"
                     /label=EosFP
                     /translation="SAIKPDMKINLRMEGNVNGHHFVIDGDGTGKPFEGKQSMDLEVKE
                     GGPLPFAFDILTTAFHYGNRVFAEYPDHIQDYFKQSFPKGYSWERSLTFEDGGICIARN
                     DITMEGDTFYNKVRFHGVNFPANGPVMQKKTLKWEPSTEKMYVRDGVLTGDIRMALLLE
                     GNAHYRCDFRTTYKAKEKGVKLPGYHFVDHCIEILSHDKDYNKVKLYEHAVAHSGLPDN
                     ARR"
     rep_origin      complement(10132..10587)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10269..10290)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     10481..10500
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        10614..10718
                     /gene="bla"
                     /label=AmpR promoter
     promoter        10720..11077
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        10720..10911
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(10743..10763)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      10928..11063
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             11112..11906
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             11112..11906
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(11166..11185)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11776..11795
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(12094..12113)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    12138..12185
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      12514..13102
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     13003..13022
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgtggata accgtattac cgccatgcat tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
      421 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
      481 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
      541 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctgg
      601 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatgagtgc gattaagcca
      661 gacatgaaga tcaacctccg tatggaaggc aacgtaaacg ggcaccactt tgtgatcgac
      721 ggagatggta caggcaagcc ttttgaggga aaacagagta tggatcttga agtcaaagag
      781 ggcggacctc tgccttttgc ctttgatatc ctgaccactg cattccatta cggcaacagg
      841 gtattcgccg aatatccaga ccacatacaa gactatttta agcagtcgtt tcctaagggg
      901 tattcgtggg aacgaagctt gactttcgaa gacgggggca tttgcattgc cagaaacgac
      961 ataacaatgg aaggggacac tttctataat aaagttcgat ttcacggtgt aaactttccc
     1021 gccaatggtc cagttatgca gaagaagacg ctgaaatggg agccctccac tgagaaaatg
     1081 tatgtgcgtg atggagtgct gactggtgat attcgcatgg ctttgttgct tgaaggaaat
     1141 gcccattacc gatgtgactt cagaactact tacaaagcta aggagaaggg tgtcaagtta
     1201 ccaggctacc actttgtgga ccactgcatt gagattttaa gccatgacaa agattacaac
     1261 aaggttaagc tgtatgagca tgctgttgct cattctggat tgcctgacaa tgccagacga
     1321 tccggtagcg gtcacggtac tggttctact ggttctggtt cttctgagag tgcgattaag
     1381 ccagacatga agatcaacct ccgtatggaa ggcaacgtaa acgggcacca ctttgtgatc
     1441 gacggagatg gtacaggcaa gccttttgag ggaaaacaga gtatggatct tgaagtcaaa
     1501 gagggcggac ctctgccttt tgcctttgat atcctgacca ctgcattcca ttacggcaac
     1561 agggtattcg ccgaatatcc agaccacata caagactatt ttaagcagtc gtttcctaag
     1621 gggtattcgt gggaacgaag cttgactttc gaagacgggg gcatttgcat tgccagaaac
     1681 gacataacaa tggaagggga cactttctat aataaagttc gatttcacgg tgtaaacttt
     1741 cccgccaatg gtccagttat gcagaagaag acgctgaaat gggagccctc cactgagaaa
     1801 atgtatgtgc gtgatggagt gctgactggt gatattcgca tggctttgtt gcttgaagga
     1861 aatgcccatt accgatgtga cttcagaact acttacaaag ctaaggagaa gggtgtcaag
     1921 ttaccaggct accactttgt ggaccactgc attgagattt taagccatga caaagattac
     1981 aacaaggtta agctgtatga gcatgctgtt gctcattctg gattgcctga caatgccaga
     2041 cgatccggag ggagcggggc tggagggagc gggggtggca gcggtagtag ctcccactct
     2101 cgggcgggcc agagcgcagc aggcgcggct ccgggcggcg gcgtcgacac gcgggacgcc
     2161 gagatgccgg ccaccgagaa ggacctggcg gaggacgcgc catggaagaa gatccagcag
     2221 aacactttca cgcgctggtg caacgagcac ctgaagtgcg tgagcaagcg catcgccaac
     2281 ctgcagacgg acctgagcga cgggctgcgg cttatcgcgc tgttggaggt gctcagccag
     2341 aagaagatgc accgcaagca caaccagcgg cccactttcc gccaaatgca gcttgagaac
     2401 gtgtcggtgg cactcgagtt cctggaccgc gagagcatca aactggtgtc catcgacagc
     2461 aaggccatcg tggacgggaa cctgaagctg atcctgggcc tcatctggac cctgatcctg
     2521 cactactcca tctccatgcc catgtgggac gaggaggagg atgaggaggc caagaagcag
     2581 acccccaagc agaggctcct gggctggatc cagaacaagc tgccgcagct gcccatcacc
     2641 aacttcagcc gggactggca gagcggccgg gccctgggcg ccctggtgga cagctgtgcc
     2701 ccgggcctgt gtcctgactg ggactcttgg gacgccagca agcccgttac caatgcgcga
     2761 gaggccatgc agcaggcgga tgactggctg ggcatccccc aggtgatcac ccccgaggag
     2821 attgtggacc ccaacgtgga cgagcactct gtcatgacct acctgtccca gttccccaag
     2881 gccaagctga agccaggggc tcccttgcgg cccaaactga acccgaagaa agcccgtgcc
     2941 tacgggccag gcatcgagcc cacaggcaac atggtgaaga agcgggcaga gttcactgtg
     3001 gagaccagaa gtgctggcca gggagaggtg ctggtgtacg tggaggaccc ggccggacac
     3061 caggaggagg caaaagtgac cgccaataac gacaagaacc gcaccttctc cgtctggtac
     3121 gtccccgagg tgacggggac tcataaggtt actgtgctct ttgctggcca gcacatcgcc
     3181 aagagcccct tcgaggtgta cgtggataag tcacagggtg acgccagcaa agtgacagcc
     3241 caaggtcccg gcctggagcc cagtggcaac atcgccaaca agaccaccta ctttgagatc
     3301 tttacggcag gagctggcac gggcgaggtc gaggttgtga tccaggaccc catgggacag
     3361 aagggcacgg tagagcctca gctggaggcc cggggcgaca gcacataccg ctgcagctac
     3421 cagcccacca tggagggcgt ccacaccgtg cacgtcacgt ttgccggcgt gcccatccct
     3481 cgcagcccct acactgtcac tgttggccaa gcctgtaacc cgagtgcctg ccgggcggtt
     3541 ggccggggcc tccagcccaa gggtgtgcgg gtgaaggaga cagctgactt caaggtgtac
     3601 acaaagggcg ctggcagtgg ggagctgaag gtcaccgtga agggccccaa gggagaggag
     3661 cgcgtgaagc agaaggacct gggggatggc gtgtatggct tcgagtatta ccccatggtc
     3721 cctggaacct atatcgtcac catcacgtgg ggtggtcaga acatcgggcg cagtcccttc
     3781 gaagtgaagg tgggcaccga gtgtggcaat cagaaggtac gggcctgggg ccctgggctg
     3841 gagggcggcg tcgttggcaa gtcagcagac tttgtggtgg aggctatcgg ggacgacgtg
     3901 ggcacgctgg gcttctcggt ggaagggcca tcgcaggcta agatcgaatg tgacgacaag
     3961 ggcgacggct cctgtgatgt gcgctactgg ccgcaggagg ctggcgagta tgccgttcac
     4021 gtgctgtgca acagcgaaga catccgcctc agccccttca tggctgacat ccgtgacgcg
     4081 ccccaggact tccacccaga cagggtgaag gcacgtgggc ctggattgga gaagacaggt
     4141 gtggccgtca acaagccagc agagttcaca gtggatgcca agcacggtgg caaggcccca
     4201 cttcgggtcc aagtccagga caatgaaggc tgccctgtgg aggcgttggt caaggacaac
     4261 ggcaatggca cttacagctg ctcctacgtg cccaggaagc cggtgaagca cacagccatg
     4321 gtgtcctggg gaggcgtcag catccccaac agccccttca gggtgaatgt gggagctggc
     4381 agccacccca acaaggtcaa agtatacggc cccggagtag ccaagacagg gctcaaggcc
     4441 cacgagccca cctacttcac tgtggactgc gccgaggctg gccaggggga cgtcagcatc
     4501 ggcatcaagt gtgcccctgg agtggtaggc cccgccgaag ctgacatcga cttcgacatc
     4561 atccgcaatg acaatgacac cttcacggtc aagtacacgc cccggggggc tggcagctac
     4621 accattatgg tcctctttgc tgaccaggcc acgcccacca gccccatccg agtcaaggtg
     4681 gagccctctc atgacgccag taaggtgaag gccgagggcc ctggcctcag tcgcactggt
     4741 gtcgagcttg gcaagcccac ccacttcaca gtaaatgcca aagctgctgg caaaggcaag
     4801 ctggacgtcc agttctcagg actcaccaag ggggatgcag tgcgagatgt ggacatcatc
     4861 gaccaccatg acaacaccta cacagtcaag tacacgcctg tccagcaggg tccagtaggc
     4921 gtcaatgtca cttatggagg ggatcccatc cctaagagcc ctttctcagt ggcagtatct
     4981 ccaagcctgg acctcagcaa gatcaaggtg tctggcctgg gagagaaggt ggacgttggc
     5041 aaagaccagg agttcacagt caaatcaaag ggtgctggtg gtcaaggcaa agtggcatcc
     5101 aagattgtgg gcccctcggg tgcagcggtg ccctgcaagg tggagccagg cctgggggct
     5161 gacaacagtg tggtgcgctt cctgccccgt gaggaagggc cctatgaggt ggaggtgacc
     5221 tatgacggcg tgcccgtgcc tggcagcccc tttcctctgg aagctgtggc ccccaccaag
     5281 cctagcaagg tgaaggcgtt tgggccgggg ctgcagggag gcagtgcggg ctcccccgcc
     5341 cgcttcacca tcgacaccaa gggcgccggc acaggtggcc tgggcctgac ggtggagggc
     5401 ccctgtgagg cgcagctcga gtgcttggac aatggggatg gcacatgttc cgtgtcctac
     5461 gtgcccaccg agcccgggga ctacaacatc aacatcctct tcgctgacac ccacatccct
     5521 ggctccccat tcaaggccca cgtggttccc tgctttgacg catccaaagt caagtgctca
     5581 ggccccgggc tggagcgggc caccgctggg gaggtgggcc aattccaagt ggactgctcg
     5641 agcgcgggca gcgcggagct gaccattgag atctgctcgg aggcggggct tccggccgag
     5701 gtgtacatcc aggaccacgg tgatggcacg cacaccatta cctacattcc cctctgcccc
     5761 ggggcctaca ccgtcaccat caagtacggc ggccagcccg tgcccaactt ccccagcaag
     5821 ctgcaggtgg aacctgcggt ggacacttcc ggtgtccagt gctatgggcc tggtattgag
     5881 ggccagggtg tcttccgtga ggccaccact gagttcagtg tggacgcccg ggctctgaca
     5941 cagaccggag ggccgcacgt caaggcccgt gtggccaacc cctcaggcaa cctgacggag
     6001 acctacgttc aggaccgtgg cgatggcatg tacaaagtgg agtacacgcc ttacgaggag
     6061 ggactgcact ccgtggacgt gacctatgac ggcagtcccg tgcccagcag ccccttccag
     6121 gtgcccgtga ccgagggctg cgacccctcc cgggtgcgtg tccacgggcc aggcatccaa
     6181 agtggcacca ccaacaagcc caacaagttc actgtggaga ccaggggagc tggcacgggc
     6241 ggcctgggcc tggctgtaga gggcccctcc gaggccaaga tgtcctgcat ggataacaag
     6301 gacggcagct gctcggtcga gtacatccct tatgaggctg gcacctacag cctcaacgtc
     6361 acctatggtg gccatcaagt gccaggcagt cctttcaagg tccctgtgca tgatgtgaca
     6421 gatgcgtcca aggtcaagtg ctctgggccc ggcctgagcc caggcatggt tcgtgccaac
     6481 ctccctcagt ccttccaggt ggacacaagc aaggctggtg tggccccatt gcaggtcaaa
     6541 gtgcaagggc ccaaaggcct ggtggagcca gtggacgtgg tagacaacgc tgatggcacc
     6601 cagaccgtca attatgtgcc cagccgagaa gggccctaca gcatctcagt actgtatgga
     6661 gatgaagagg taccccggag ccccttcaag gtcaaggtgc tgcctactca tgatgccagc
     6721 aaggtgaagg ccagtggccc cgggctcaac accactggcg tgcctgccag cctgcccgtg
     6781 gagttcacca tcgatgcaaa ggacgccggg gagggcctgc tggctgtcca gatcacggat
     6841 cccgaaggca agccgaagaa gacacacatc caagacaacc atgacggcac gtatacagtg
     6901 gcctacgtgc cagacgtgac aggtcgctac accatcctca tcaagtacgg tggtgacgag
     6961 atccccttct ccccgtaccg cgtgcgtgcc gtgcccaccg gggacgccag caagtgcact
     7021 gtcacaggtg ctggcatcgg ccccaccatt cagattgggg aggagacggt gatcactgtg
     7081 gacactaagg cggcaggcaa aggcaaagtg acgtgcaccg tgtgcacgcc tgatggctca
     7141 gaggtggatg tggacgtggt ggagaatgag gacggcactt tcgacatctt ctacacggcc
     7201 ccccagccgg gcaaatacgt catctgtgtg cgctttggtg gcgagcacgt gcccaacagc
     7261 cccttccaag tgacggctct ggctggggac cagccctcgg tgcagccccc tctacggtct
     7321 cagcagctgg ccccacagta cacctacgcc cagggcggcc agcagacttg ggccccggag
     7381 aggcccctgg tgggtgtcaa tgggctggat gtgaccagcc tgaggccctt tgaccttgtc
     7441 atccccttca ccatcaagaa gggcgagatc acaggggagg ttcggatgcc ctcaggcaag
     7501 gtggcgcagc ccaccatcac tgacaacaaa gacggcaccg tgaccgtgcg gtatgcaccc
     7561 agcgaggctg gcctgcacga gatggacatc cgctatgaca acatgcacat cccaggaagc
     7621 cccttgcagt tctatgtgga ttacgtcaac tgtggccatg tcactgccta tgggcctggc
     7681 ctcacccatg gagtagtgaa caagcctgcc accttcaccg tcaacaccaa ggatgcagga
     7741 gaggggggcc tgtctctggc cattgagggc ccgtccaaag cagaaatcag ctgcactgac
     7801 aaccaggatg ggacatgcag cgtgtcctac ctgcctgtgc tgccggggga ctacagcatt
     7861 ctagtcaagt acaatgaaca gcacgtccca ggcagcccct tcactgcccg ggtcacaggt
     7921 gacgactcca tgcgtatgtc ccacctaaag gtcggctctg ctgccgacat ccccatcaac
     7981 atctcagaga cggatctcag cctgctgacg gccactgtgg tcccgccctc gggccgggag
     8041 gagccctgtt tgctgaagcg gctgcgtaat ggccacgtgg ggatttcatt cgtgcccaag
     8101 gagacggggg agcacctggt gcatgtgaag aaaaatggcc agcacgtggc cagcagcccc
     8161 atcccggtgg tgatcagcca gtcggaaatt ggggatgcca gtcgtgttcg ggtctctggt
     8221 cagggccttc acgaaggcca cacctttgag cctgcagagt ttatcattga tacccgcgat
     8281 gcaggctatg gtgggctcag cctgtccatt gagggcccca gcaaggtgga catcaacaca
     8341 gaggacctgg aggacgggac gtgcagggtc acctactgcc ccacagagcc aggcaactac
     8401 atcatcaaca tcaagtttgc cgaccagcac gtgcctggca gccccttctc tgtgaaggtg
     8461 acaggcgagg gccgggtgaa agagagcatc acccgcaggc gtcgggctcc ttcagtggcc
     8521 aacgttggta gtcattgtga cctcagcctg aaaatccctg aaattagcat ccaggatatg
     8581 acagcccagg tgaccagccc atcgggcaag acccatgagg ccgagatcgt ggaaggggag
     8641 aaccacacct actgcatccg ctttgttccc gctgagatgg gcacacacac agtcagcgtc
     8701 aagtacaagg gccagcacgt gcctgggagc cccttccagt tcaccgtggg gcccctaggg
     8761 gaagggggag cccacaaggt ccgagctggg ggccctggcc tggagagagc tgaagctgga
     8821 gtgccagccg aattcagtat ctggacccgg gaagctggtg ctggaggcct ggccattgct
     8881 gtcgagggcc ccagcaaggc tgagatctct tttgaggacc gcaaggacgg ctcctgtggt
     8941 gtggcttatg tggtccagga gccaggtgac tacgaagtct cagtcaagtt caacgaggaa
     9001 cacattcccg acagcccctt cgtggtgcct gtggcttctc cgtctggcga cgcccgccgc
     9061 ctcactgttt ctagccttca ggagtcaggg ctaaaggtca accagccagc ctcttttgca
     9121 gtcagcctga acggggccaa gggggcgatc gatgccaagg tgcacagccc ctcaggagcc
     9181 ctggaggagt gctatgtcac agaaattgac caagataagt atgctgtgcg cttcatccct
     9241 cgggagaatg gcgtttacct gattgacgtc aagttcaacg gtacccacat ccctggaagc
     9301 cccttcaaga tccgagttgg ggagcctggg catggagggg acccaggctt ggtgtctgct
     9361 tacggagcag gtctggaagg cggtgtcaca gggaacccag ctgagttcgt cgtgaacacg
     9421 agcaatgcgg gagctggtgc cctgtcggtg accattgacg gcccctccaa ggtgaagatg
     9481 gattgccagg agtgccctga gggctaccgc gtcacctata cccccatggc acctggcagc
     9541 tacctcatct ccatcaagta cggcggcccc taccacattg ggggcagccc cttcaaggcc
     9601 aaagtcacag gcccccgtct cgtcagcaac cacagcctcc acgagacatc atcagtgttt
     9661 gtagactctc tgaccaaggc cacctgtgcc ccccagcatg gggccccggg tcctgggcct
     9721 gctgacgcca gcaaggtggt ggccaagggc ctggggctga gcaaggccta cgtaggccag
     9781 aagagcagct tcacagtaga ctgcagcaaa gcaggcaaca acatgctgct ggtgggggtt
     9841 catggcccaa ggaccccctg cgaggagatc ctggtgaagc acgtgggcag ccggctctac
     9901 agcgtgtcct acctgctcaa ggacaagggg gagtacacac tggtggtcaa atgggggcac
     9961 gagcacatcc caggcagccc ctaccgcgtt gtggtgccct aaaagcttcg aattctgcag
    10021 tcgacggtac cgcgggcccg ggatccaccg gatctagata actgatcata atcagccata
    10081 ccacatttgt agwkgtkktw yktccaawcw catcaatgta tcttaacgcg taaattgtaa
    10141 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
    10201 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
    10261 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
    10321 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
    10381 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
    10441 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
    10501 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
    10561 cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa
    10621 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    10681 cctgataaat gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
    10741 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
    10801 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
    10861 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
    10921 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
    10981 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
    11041 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
    11101 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
    11161 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
    11221 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
    11281 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
    11341 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
    11401 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
    11461 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
    11521 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
    11581 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
    11641 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
    11701 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
    11761 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
    11821 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
    11881 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
    11941 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
    12001 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
    12061 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
    12121 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
    12181 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
    12241 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
    12301 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
    12361 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12421 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12481 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    12541 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    12601 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    12661 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    12721 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    12781 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
    12841 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
    12901 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
    12961 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
    13021 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
    13081 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
    13141 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgat
//
