LOCUS       sequence-235413-        1364 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Chimeric ETS domain: H2 of Ets-1 in PU.1 scaffold.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Albrecht AV, Kim HM, Poon GMK
  TITLE     Mapping interfacial hydration in ETS-family transcription factor 
            complexes with DNA: a chimeric approach.
  JOURNAL   Nucleic Acids Res. 2018 Nov 16;46(20):10577-10588. doi: 
            10.1093/nar/gky894.
  PUBMED    30295801
REFERENCE   2  (bases 1 to 1364)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nucleic Acids Res"; date: "2018-11-16- 16"; volume: "46"; issue: 
            "20"; pages: "10577-10588"
COMMENT     Sequence Label: pET28b-PU.1-ETS-H2
FEATURES             Location/Qualifiers
     source          1..1364
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             367..384
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             406..423
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(476..494)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      490..537
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      574..1029
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(661..680)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     871..892
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     gap             1349..1362
                     /estimated_length=14
ORIGIN
        1 tnnnnnctag antaattttg ttnaacttta agaaggagat ataccatggg atcgaagaaa
       61 aaaattcgct tatatcagtt tttacttgac ctgctgcgta gtggcgacat gaaagattct
      121 atttggtggg tggacaaaga taaggggacc tttcagttct cgtcaaaaga tcctgatgag
      181 gtggcacgcc gttgggggat tcaaaagggt aaccgcaaaa aaatgactta tcagaagatg
      241 gcgcgtgccc ttcgcaatta tggtaaaacc ggggaggtca aaaaggtaaa gaaaaagttg
      301 acctatcagt ttagtggaga agtattaggg cgtgggggtt tggcagagcg ccgcctgcct
      361 ccacacttgg ttccacgtgg gagcaagctt gcggccgcac tcgagcacca ccaccaccac
      421 cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct
      481 gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg
      541 aaaggaggaa ctatatccgg attggcgaat gggacgcgcc ctgtagcggc gcattaagcg
      601 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
      661 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
      721 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
      781 ancttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
      841 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
      901 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
      961 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     1021 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     1081 ctaaatacat tcaaatatgt atccgctcat gaattaattc ttagaaaanc tcatcgagca
     1141 tcaantgaaa ctgnnattta ttcanatcag gattatcnnt accanntttt tgaaaaagcc
     1201 ntttnngtaa tgaaggaaaa annnnnccga ggcagttcct taggatggna agatcctggn
     1261 atcggnctgg naatccgaan nnnnnaantc aanncaaccn nttaatttcc ccnnncnnaa
     1321 aaanggntta ccagggnaaa annccnntnn nnnnnnnnnn nntc
//
