LOCUS       sequence-235835-         696 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses WT GgKDELR2 in S. cerevisiae cells using Galactose 
            induction, -URA and -Leu selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Brauer P, Parker JL, Gerondopoulos A, Zimmermann I, Seeger MA, Barr 
            FA, Newstead S
  TITLE     Structural basis for pH-dependent retrieval of ER proteins from the 
            Golgi by the KDEL receptor.
  JOURNAL   Science. 2019 Mar 8;363(6431):1103-1107. doi: 
            10.1126/science.aaw2859.
  PUBMED    30846601
REFERENCE   2  (bases 1 to 696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2019-03-8- 8"; volume: "363"; issue: 
            "6431"; pages: "1103-1107"
COMMENT     Sequence Label: pDDGFP-Leu2d-GgKDELR2
FEATURES             Location/Qualifiers
     source          1..696
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     10..26
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     10..26
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             670..690
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
ORIGIN
        1 accccggatt ctagaactag tggatccccc atgaacatct tcagattgac cggtgatttg
       61 tctcatttgg ctgccattat tatcctgttg ttgaagatct ggaagtctag atcttgtgct
      121 ggtatttctg gtaagtctca gttgttgttt gctttggttt tcaccaccag atacttggat
      181 ttgttcacct ccttcatcag cttgtacaac acttctatga agctgatcta cattgcttgt
      241 tcttacgcta ccgtttacct gatctatatg aagttcaagg ctacctacga tggtaaccat
      301 gatactttca gagtcgaatt cttgatcgtt ccagttggtg gtttgtcttt cttggttaat
      361 cacgatttct ccccattgga aattttgtgg actttctcca tctacttgga atccgttgct
      421 attttgccac agttgttcat gatttctaag accggtgaag ctgaaaccat tactactcac
      481 tacttgtttt tcttgggctt gtatagagcc ttgtacttgg ttaactggat ttggaggtat
      541 tacttcgaag gtttcttcga tttgattgct gttgttgctg gtgttgttca aactgtcttg
      601 tactgtgatt tcttctactt gtacgttacc aaggtcttga agggtaagaa attgtctttg
      661 ccagctgggg aaaatttata ttttcaaggt caattt
//
