LOCUS       sequence-235866-        9094 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression, Genome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9094)
  AUTHORS   Teng F, Li J, Cui T, Xu K, Guo L, Gao Q, Feng G, Chen C, Han D, Zhou
            Q, Li W
  TITLE     Enhanced mammalian genome editing by new Cas12a orthologs with 
            optimized crRNA scaffolds.
  JOURNAL   Genome Biol. 2019 Feb 5;20(1):15. doi: 10.1186/s13059-019-1620-8.
  PUBMED    30717767
REFERENCE   2  (bases 1 to 9094)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2019-02-5"; volume: "20(1):15. doi"; pages: " 
            10.1186/s13059-019-1620-8"
COMMENT     Sequence Label: pCAG-ArCas12a-2AeGFP
FEATURES             Location/Qualifiers
     source          1..9094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        8..387
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        390..665
                     /label=chicken beta-actin promoter
     intron          666..1678
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1602..1624
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1686..1705
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1739..1757
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1769..1858
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
     CDS             1865..1885
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5684..5704
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5726..5779
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(5780..5782,5783..5785,5786..6499)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5780..5782,5783..5785,5786..6496)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5825..5846)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6086..6105)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6433..6454
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6542..6663
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6579..6598)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6633..6652
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6706..6722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6706..6722)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6719..6741)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6730..6746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6754..6760,6761..6778,6779..6784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6799..6820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6937..6954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7108..7696)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7188..7207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7867..8658,8659..8727))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8490..8509
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8728..8832)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8911..8930)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 acgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
       61 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      121 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      181 atagggactt tccattgacg tcaatgggtg gactatttac ggtaaactgc ccacttggca
      241 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
      301 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
      361 tacgtattag tcatcgctat taccatgggt cgaggtgagc cccacgttct gcttcactct
      421 ccccatctcc cccccctccc cacccccaat tttgtattta tttatttttt aattattttg
      481 tgcagcgatg ggggcggggg gggggggggc gcgcgccagg cggggcgggg cggggcgagg
      541 ggcggggcgg ggcgaggcgg agaggtgcgg cggcagccaa tcagagcggc gcgctccgaa
      601 agtttccttt tatggcgagg cggcggcggc ggcggcccta taaaaagcga agcgcgcggc
      661 gggcgggagt cgctgcgttg ccttcgcccc gtgccccgct ccgcgccgcc tcgcgccgcc
      721 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
      781 ctccgggctg taattagcgc ttggtttaat gacggctcgt ttcttttctg tggctgcgtg
      841 aaagccttaa agggctccgg gagggccctt tgtgcggggg ggagcggctc ggggggtgcg
      901 tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggcccgc gctgcccggc ggctgtgagc
      961 gctgcgggcg cggcgcgggg ctttgtgcgc tccgcgtgtg cgcgagggga gcgcggccgg
     1021 gggcggtgcc ccgcggtgcg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg
     1081 tgcgtggggg ggtgagcagg gggtgtgggc gcggcggtcg ggctgtaacc cccccctgca
     1141 cccccctccc cgagttgctg agcacggccc ggcttcgggt gcggggctcc gtacggggcg
     1201 tggcgcgggg ctcgccgtgc cgggcggggg gtggcggcag gtgggggtgc cgggcggggc
     1261 ggggccgcct cgggccgggg agggctcggg ggaggggcgc ggcggccccc ggagcgccgg
     1321 cggctgtcga ggcgcggcga gccgcagcca ttgcctttta tggtaatcgt gcgagagggc
     1381 gcagggactt cctttgtccc aaatctgtgc ggagccgaaa tctgggaggc gccgccgcac
     1441 cccctctagc gggcgcgggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga
     1501 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgtcc
     1561 gcagggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     1621 gaccggcggc tctagcgcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     1681 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagcta gtgaattcta
     1741 atacgactca ctataggccg ccaccatgta cccttatgat gttccagatt atgccggata
     1801 cccatacgat gtccctgact atgcaggctc ctacccttat gacgtcccag actacgccac
     1861 cggtcccaag aagaagagga aggttcccgg gaacaacggc acaaacaatt tccagaactt
     1921 cattggcatt tcatcactcc agaagaccct gaggaatgcc ctgacaccta ccgagacaac
     1981 ccagcaattc attgtgaaga acggaatcat taaggaggac gaactgagag gcgagaaccg
     2041 ccagatcctg aaagacatca tggacgacta ctatcgcggt ttcatctccg agacactcag
     2101 cagcattgat gatattgatt ggacatctct gtttgagaag atggaaatcc agctgaagaa
     2161 tggcgacaac aaggacactc tcattaagga gcaagcagag aagagaaagg ctatctacaa
     2221 gaagtttgca gacgacgatc gcttcaagaa tatgtttagc gcaaagctga taagtgatat
     2281 tctcccagag tttgtgattc acaacaacaa ctattctgcc agcgagaagg aagagaagac
     2341 acaggtcatc aagctcttct ctcggtttgc cacctcattc aaagattact tcaagaatcg
     2401 ggcaaattgt ttctctgccg atgacatctc atcttcctcc tgtcacagaa tcgttaatga
     2461 taacgccgaa atcttcttct caaatgccct ggtgtaccgg agaatcgtga agaatctgag
     2521 taacgacgac atcaataaga ttagcggtga tatgaaggac tctctgaaga agatgtccct
     2581 ggagaagata tacagctatg agaagtacgg cgagttcatc actcaagagg gaattagctt
     2641 ctacaacgac atttgtggca aggttaactc attcatgaat ctctactgcc agaagaacaa
     2701 ggagaataag aatctgtaca aactgcgcaa actgcataag caaatcctgt gtatcgccga
     2761 cacctcttat gaggtgccct acaagtttga gtctgacgaa gaggtgtacc agtccgtgaa
     2821 cggattcctg gacaacatca gttctaaaca catcgtcgag cggctgagga agatcggcga
     2881 caactacaat ggctataacc tggacaagat atacatcgtc agtaagttct atgaaagtgt
     2941 gagtcagaag acctaccggg actgggagac tatcaacacc gcactcgaaa tccactacaa
     3001 taacatcctg cctggcaacg gtaagagcaa ggccgacaag gtgaagaagg ccgtcaagaa
     3061 cgacctccag aagagcatca ccgagattaa cgaactggtg agtaactaca agctctgccc
     3121 agacgataac attaaggcag aaacctacat tcatgagatt tcccatatac tgaacaactt
     3181 tgaagctcaa gagctgaaat acaatcccga gattcatctg gtggagagcg agctgaaagc
     3241 atccgagctg aagaacgttc tcgacgtcat catgaacgcc ttccactggt gtagcgtgtt
     3301 catgactgag gagctggtcg ataaagacaa caatttctac gccgagctgg aagaaatcta
     3361 cgacgagata taccctgtga ttagcctcta caatctggtc cggaactatg tgacccagaa
     3421 gccctattca actaagaaga tcaagctgaa ctttgggatt cctaccctgg ccgacggctg
     3481 gtccaagagc aaagagtatt ccaataacgc aatcatcctg atgagagaca acctgtatta
     3541 cctcggtatc tttaacgcta agaataagcc cgagaagaag atcatcgaag gaaatacatc
     3601 cgagaacaag ggcgactaca agaagatgat ctataacctg ctgcctggcc caaacaagat
     3661 gatccctaag gtgttcctga gcagcaagac cggagtcgag acttacaagc caagtgccta
     3721 catactggag ggctataagc agaacaagca cctgaaatct agcaaagatt tcgacatcac
     3781 tttctgtcgc gacctgatcg actatttcaa gaattgtatt gccatccacc cagagtggaa
     3841 gaatttcgga ttcgacttct ctgacacctc cacatacgag gacatcagtg gcttctatag
     3901 agaagtggag ctgcaaggtt acaagatcga ctggacctac atatctgaga aagacatcga
     3961 cctgctgcaa gagaaagggc agctctacct cttccaaatc tacaacaagg actttagtaa
     4021 gaagtctaca ggtaatgaca atctgcacac tatgtacctg aagaatctct tctctgaaga
     4081 gaacctcaaa gacgtggtgc tgaaactgaa cggcgaagca gaaatcttct ttcgcaaatc
     4141 atccattaag aatcctatca tacacaagaa gggtagtatc ctggtgaaca ggacatacga
     4201 agccgaggag aaagatcagt tcggcaacat tcagattgtg cgcaagacta ttcccgagaa
     4261 tatctaccag gagctgtaca aatacttcaa cgataagtct gataaagagc tgtcagacga
     4321 agcagccaag ctgaagaatg ctgtgggaca tcatgaagca gctactaaca tcgtgaaaga
     4381 ctatagatac acatacgaca agtatttcct gcacatgcca attaccatca acttcaaagc
     4441 caataagact tctttcatta acgaccgcat cctccagtac attgcaaagg agaaagacct
     4501 gcacgtgatc gggattgatc gcggagaacg gaacctcatc tacgtttcag tcatcgacac
     4561 atgcggtaac atcgtcgaac agaagagctt caacattgtt aatgggtatg attaccagat
     4621 caaactcaag caacaggaag gcgcacggca gattgctcgc aaggagtgga aggaaattgg
     4681 caagatcaag gaaatcaagg aaggatacct cagcctcgtc attcatgaaa tcagcaagat
     4741 ggtgatcaag tataacgcaa tcatcgctat ggaggacctg agttatggct tcaagaaagg
     4801 cagattcaag gtggagcggc aagtctacca gaaattcgaa acaatgctga tcaacaagct
     4861 gaactacctg gtgttcaaag acatcagcat aaccgagaac ggaggactcc tgaaaggcta
     4921 ccagctcaca tacatcccag agaaactcaa gaatgtgggc caccaatgcg gctgcatctt
     4981 ctacgtccct gccgcttaca ccagcaagat agatcccact acaggattcg tgaacatatt
     5041 caaattcaag gatctgacag tggacgccaa gagggagttc atcaagaagt ttgatagtat
     5101 tcgctatgac agcgataaga atctgttctg tttcaccttt gactacaaca acttcattac
     5161 ccagaatacc gttatgtcca agtctagctg gagtgtgtat acctacggtg ttcggatcaa
     5221 gcggaggttt gtcaatggta gattctcaaa cgaaagcgac accatcgaca tcacaaagga
     5281 catggagaag acactggaaa tgactgacat aaactggaga gatggacacg acctgcggca
     5341 agacatcatt gactacgaga tcgttcagca catctttgaa atcttcaagc tgactgttca
     5401 gatgcggaat agtctgagcg agctggagga ccggaattac gaccgcctga tctcaccagt
     5461 cctgaacgag aataacatct tctacgattc tgccaaagca ggagatgccc tgccaaagga
     5521 cgctgatgca aacggtgcct actgcatcgc cctcaaaggt ctgtacgaaa tcaagcagat
     5581 taccgagaat tggaaggagg acgggaagtt cagcagagac aagctcaaga tcagcaataa
     5641 ggactggttc gatttcattc agaacaagcg ctacctgact agcccaaaga agaagaggaa
     5701 agtctctaga aggtccggcg gcggagaggg cagaggaagt cttctaacat gcggtgacgt
     5761 ggaggagaat cccggcccaa tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     5821 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     5881 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     5941 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     6001 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     6061 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     6121 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     6181 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     6241 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     6301 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     6361 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     6421 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     6481 ggacgagctg tacaagtaac tgcagcgcgg ggatctcatg ctggagttct tcgcccaccc
     6541 caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     6601 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     6661 ttattaatta acgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc
     6721 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     6781 taaagcctag ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     6841 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     6901 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     6961 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     7021 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     7081 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     7141 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     7201 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     7261 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     7321 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     7381 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     7441 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     7501 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg
     7561 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     7621 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     7681 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     7741 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     7801 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     7861 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     7921 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     7981 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     8041 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     8101 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     8161 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     8221 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     8281 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     8341 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     8401 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     8461 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     8521 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     8581 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     8641 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     8701 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     8761 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     8821 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatcg
     8881 atctcccgat cccctagggt cgactctcag tacaatctgc tctgatgccg catagttaag
     8941 ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga gcaaaattta
     9001 agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta gggttaggcg
     9061 ttttgcgctg cttcgcgatg tacgggccag atat
//
