LOCUS       sequence-235887-        8715 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  doxycycline-inducible mouse Wnt3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8715)
  AUTHORS   Wang Q, Zou Y, Nowotschin S, Kim SY, Li QV, Soh CL, Su J, Zhang C, 
            Shu W, Xi Q, Huangfu D, Hadjantonakis AK, Massague J
  TITLE     The p53 Family Coordinates Wnt and Nodal Inputs in Mesendodermal 
            Differentiation of Embryonic Stem Cells.
  JOURNAL   Cell Stem Cell. 2017 Jan 5;20(1):70-86. doi: 
            10.1016/j.stem.2016.10.002. Epub 2016 Nov 23.
  PUBMED    27889317
REFERENCE   2  (bases 1 to 8715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2016.10.002"; journalName: "Cell Stem Cell"; date: 
            "2017-01-5- 5"; volume: "20"; issue: "1"; pages: "70-86"
COMMENT     Sequence Label: pLVXT-mWnt3
FEATURES             Location/Qualifiers
     source          1..8715
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..116
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     protein_bind    187..205
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    223..241
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    259..277
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    295..313
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    331..349
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        361..399
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     396..420
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      437..446
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        1521..2020
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1556..1579)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1953..1972
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2041..2640
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2041..2060)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2537..2557
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2657..3245
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2710..2730)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3128..3139)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3452..4085
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4213..4229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4213..4229)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4226..4248)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4237..4253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4261..4267,4268..4285,4286..4291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4306..4327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4444..4461)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4615..5203)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4695..4714)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5374..6165,6166..6234))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5997..6016
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6235..6339)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6387..6521
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6424..6443)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6478..6497
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6689..7322
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7369..7494
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7991..8224
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8409..8453
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt agacataata
       61 gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca aaattttcgg
      121 gtttattaca gggacagcag agatccagtt tatcgatgag gccctttcgt cttcactcga
      181 gtttactccc tatcagtgat agagaacgta tgtcgagttt actccctatc agtgatagag
      241 aacgnatgtc gagtttactc cctatcagtg atagagaacg tatgtcgagt ttantcccta
      301 tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa cgtatgtcga
      361 ggtaggcgtg tacggtggga ggcctatata agcagagctc gtttagtgaa ccgtcagatc
      421 gcctggagaa ggatccgcca ccatggagcc ccacctgctc gggctgctac tcggcctcct
      481 gctcagtggc accagggtcc tcgctggcta cccaatttgg tggtccctgg ccctgggcca
      541 gcagtacaca tctctggcct cccagcctct gctctgcggc tccatcccag gcctggtccc
      601 caagcaactg cgcttctgcc gcaattacat cgagatcatg cccagcgtag cagaaggtgt
      661 gaagctgggc atccaggagt gccagcatca gttccggggc cgccggtgga actgtaccac
      721 catagatgac agcctggcca tctttgggcc tgtcttggac aaagccaccc gtgaatcggc
      781 cttcgtgcat gccatcgcct cggctggtgt cgccttcgca gtcacacgct cctgcgctga
      841 gggaacctcc accatctgcg gctgtgactc acatcataag gggccacctg gagaaggctg
      901 gaagtggggc ggctgcagcg aggacgccga cttcggggtg ctggtgtccc gggaatttgc
      961 ggatgcgcgg gagaacaggc cagatgcccg ctcagctatg aacaagcaca acaatgaagc
     1021 aggccgaacg accatcctgg accacatgca cctaaagtgt aaatgccacg ggttgtccgg
     1081 cagctgcgag gtgaagacct gctggtgggc ccagcccgac ttccgtgcca ttggcgactt
     1141 cctcaaggac aagtacgaca gtgcctccga gatggtggtg gagaaacacc gtgagtcccg
     1201 aggctgggtg gagaccctgc gggctaagta cgcgctcttc aagccaccca ccgagaggga
     1261 cctggtctac tacgagaact cccccaactt ttgtgagccc aacccagaga cgggctcctt
     1321 tggtaccagg gaccggactt gcaatgtcac ctcccacggc atcgatggct gcgatctgct
     1381 gtgctgtggc cggggccaca acacgaggac ggagaaacgg aaggagaaat gccattgcgt
     1441 cttccactgg tgctgctatg tcagctgcca agagtgtatt cgcatctacg atgtgcacac
     1501 ctgcaagtag gaattctacc gggtagggga ggcgcttttc ccaaggcagt ctggagcatg
     1561 cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc ctcgcacaca
     1621 ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct tcgcgccacc
     1681 ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt cgtgcaggac
     1741 gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca ccgctgagca
     1801 atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct tcgctttctg
     1861 ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc tcaggggcgg
     1921 ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa agcgcacgtc
     1981 tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc caagcttacc
     2041 atgaccgagt acaagcccac ggtgcgcctc gccacccgcg acgacgtccc cagggccgta
     2101 cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc gccacaccgt cgatccggac
     2161 cgccacatcg agcgggtcac cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac
     2221 atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg cggtctggac cacgccggag
     2281 agcgtcgaag cgggggcggt gttcgccgag atcggcccgc gcatggccga gttgagcggt
     2341 tcccggctgg ccgcgcagca acagatggaa ggcctcctgg cgccgcaccg gcccaaggag
     2401 cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc
     2461 agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg
     2521 gagacctccg cgccccgcaa cctccccttc tacgagcggc tcggcttcac cgtcaccgcc
     2581 gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga cccgcaagcc cggtgcctga
     2641 cgggcgcgtc tggaacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     2701 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     2761 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     2821 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     2881 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     2941 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3001 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     3061 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3121 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3181 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3241 cccgcctgga attaattctg cagtcgagac ctagaaaaac atggagcaat cacaagtagc
     3301 aatacagcag ctaccaatgc tgattgtgcc tggctagaag cacaagagga ggaggaggtg
     3361 ggttttccag tcacacctca ggtaccttta agaccaatga cttacaaggc agctgtagat
     3421 cttagccact ttttaaaaga aaagagggga ctggaagggc taattcactc ccaacgaaga
     3481 caagatatcc ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac
     3541 tacacaccag ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta
     3601 ccagttgagc cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac
     3661 cctgtgagcc tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac
     3721 agccgcctag catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc
     3781 tgatatcgag cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg
     3841 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
     3901 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     3961 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4021 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4081 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
     4141 atcagagagt gagaggcctt gacattgcta gcgtttaccg tcgacctcta gctagagctt
     4201 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     4261 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     4321 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     4381 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     4441 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     4501 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     4561 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     4621 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     4681 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     4741 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     4801 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     4861 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     4921 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     4981 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     5041 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     5101 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     5161 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     5221 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     5281 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     5341 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     5401 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     5461 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     5521 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     5581 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     5641 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     5701 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     5761 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     5821 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     5881 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     5941 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     6001 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     6061 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     6121 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     6181 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     6241 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     6301 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6361 acctgacgtc gacggatcgg gagatcaact tgtttattgc agcttataat ggttacaaat
     6421 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     6481 gtttgtccaa actcatcaat gtatcttatc atgtctggat caactggata actcaagcta
     6541 accaaaatca tcccaaactt cccaccccat accctattac cactgccaat tacctgtggt
     6601 ttcatttact ctaaacctgt gattcctctg aattattttc attttaaaga aattgtattt
     6661 gttaaatatg tactacaaac ttagtagttg gaagggctaa ttcactccca aagaagacaa
     6721 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     6781 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     6841 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     6901 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     6961 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     7021 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     7081 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     7141 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7201 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7261 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7321 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     7381 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     7441 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     7501 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     7561 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     7621 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     7681 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     7741 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     7801 caaaacaaaa gtaagaccac cgcacagcaa gcggccggcc gctgatcttc agacctggag
     7861 gaggagatat gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg
     7921 aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag
     7981 cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg
     8041 cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc
     8101 agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg
     8161 gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc
     8221 tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg
     8281 ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg
     8341 acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc
     8401 agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt
     8461 ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct
     8521 tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat
     8581 attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag
     8641 gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat
     8701 ctcgacggta tcgcc
//
