LOCUS       sequence-235968-        2783 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for deGFP (deGFP3 with 4aa 
            N-truncation, 9aa C-truncation, F64L, and C203T (numbered with 
            original sequence); see https://www.fpbase.org/protein/degfp3/).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2783)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2783)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C1m
FEATURES             Location/Qualifiers
     source          1..2783
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     305..326
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    356..376
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(382..399)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(553..1141)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(633..652)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1312..2103,2104..2172))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADNVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1935..1954
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2173..2277)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2345..2363
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(2367..2410)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    2413..2434
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     primer_bind     complement(2480..2501)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2741..2760)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
       61 tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca
      121 agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg
      181 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
      241 ccgacaacca ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg
      301 atcacatggt cctgctggag ttcgtgaccg ccgccgggat ctaaaggtag agacctacta
      361 gtagcggccg ctgcaggctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
      421 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
      481 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      541 gttgctggcg tttttccaca ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      601 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      661 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      721 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      781 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      841 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      901 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      961 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     1021 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1081 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1141 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     1201 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     1261 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     1321 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     1381 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     1441 aatgataccg cgcgacccac gctcaccggc tccagattta tcagcaataa accagccagc
     1501 cggaagggcc gagcgcagaa gtggtcctgc aacgttatcc gcctccatcc agtctattaa
     1561 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     1621 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     1681 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     1741 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     1801 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     1861 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     1921 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     1981 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     2041 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     2101 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     2161 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     2221 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     2281 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     2341 taaaaatagg cgtatcacga ggcagaattt cagataaaaa aaatccttag ctttcgctaa
     2401 ggatgatttc tggaattcgc ggccgcttct agagactagt gggtctcaaa tggagctttt
     2461 cactggcgtt gttcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag
     2521 cgtgtccggc gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg
     2581 caccaccggc aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt
     2641 gcagtgcttc agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat
     2701 gcccgaaggc tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac
     2761 ccgcgccgag gtgaagttcg agg
//
