LOCUS       sequence-235980-       10771 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TagRFP-T^SEC^BioTag::AID*::3xFLAG vector with ccdB sites for cloning
            homology arms..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Ashley GE, Duong T, Levenson MT, Martinez MAQ, Johnson LC, Hibshman 
            JD, Saeger HN, Palmisano NJ, Doonan R, Martinez-Mendez R, Davidson 
            BR, Zhang W, Ragle JM, Medwig-Kinney TN, Sirota SS, Goldstein B, 
            Matus DQ, Dickinson DJ, Reiner DJ, Ward JD
  TITLE     An expanded auxin-inducible degron toolkit for Caenorhabditis 
            elegans.
  JOURNAL   Genetics. 2021 Mar 31;217(3). pii: 6104563. doi: 
            10.1093/genetics/iyab006.
  PUBMED    33677541
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics.";
            date: "2021-03-31"; volume: "217(3). pii: 6104563. doi"; pages: " 
            10.1093/genetics/iyab006"
COMMENT     Sequence Label: pJW1594
FEATURES             Location/Qualifiers
     source          1..10771
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1923..1943
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1961..1994)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             2004..2033
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3850..4173)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6091..6115
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6118..7143
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6118..7143
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7144..7168)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7521..7554)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7696..7827
                     /codon_start=1
                     /product="minimal degron from the auxin-responsive IAA17 
                     protein of Arabidopsis thaliana (Morawska and Ulrich, 
                     2013)"
                     /label=AID*
                     /note="acts as an auxin-inducible degron (AID) in the 
                     presence of TIR1"
                     /translation="PKDPAKPPAKAQVVGWPPVRSYRKNVMVSCQKSSGGPEAAAFVK"
     CDS             7855..7878
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7882..7905
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7909..7932
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7980..8285)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8550..8566)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8550..8566)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8563..8585)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8574..8590
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8598..8604,8605..8622,8623..8628))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8643..8664
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8781..8798)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8952..9540)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9032..9051)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9711..10502,10503..10571))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10334..10353
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10572..10676)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10744..10762
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg gtctccaagg gagaggagct catcaaggag aacatgcaca
     1081 tgaagctcta catggaggga accgtcaaca accaccactt caagtgcacc tccgagggag
     1141 agggaaagcc atacgaggga acccaaacca tgcgtatcaa ggtaagttta aacatatata
     1201 tactaactaa ccctgattat ttaaattttc aggtcgtcga gggaggacca ctcccattcg
     1261 ccttcgacat cctcgccacc tccttcatgt acggatcccg taccttcatc aaccacaccc
     1321 aaggaatccc agacttcttc aagcaatcct tcccagaggg attcacctgg gagcgtgtca
     1381 ccacctacga ggacggagga gtcctcaccg ccacccaaga cacctccctc caagacggat
     1441 gcctcatcta caacgtcaag gtaagtttaa acagttcggt actaactaac catacatatt
     1501 taaattttca gatccgtgga gtcaacttcc catccaacgg accagtcatg caaaagaaga
     1561 ccctcggatg ggaggccaac accgagatgc tctacccagc cgacggagga ctcgagggac
     1621 gtaccgacat ggccctcaag ctcgtcggag gaggacacct catctgcaac ttcaagacca
     1681 cctaccgttc caaggtaagt ttaaacatga ttttactaac taactaatct gatttaaatt
     1741 ttcagaagcc agccaagaac ctcaagatgc caggagtcta ctacgtcgac caccgtctcg
     1801 agcgtatcaa ggaggccgac aaggagacct acgtcgagca acacgaggtc gccgtcgccc
     1861 gttactgcga cctcccatcc aagctcggac acaagctcaa cggaatggac gagctctaca
     1921 aggagaatct gtactttcaa tccggaaagg taagtttaaa ataacttcgt atagcataca
     1981 ttatacgaag ttattttcag ggagagcaaa agcttatctc cgaggaggac ctctaaggat
     2041 gatcgacgcc aacgtcgttg aattttcaaa ttttaaatac tgaatatttg ttttttttcc
     2101 tattatttat ttattctctt tgtgtttttt ttcttgcttt ctaaaaaatt aattcaatcc
     2161 aaatctaaac attttttttt ctctttccgt ctcccaattc gtattccgct cctctcatct
     2221 gaacacaatg tgcaagttta tttatcttct cgctttcatt tcattaggac gtggggggaa
     2281 ttggtggaag ggggaaacac acaaaaggat gatggaaatg aaataaggac acacaatatg
     2341 caacaacatt caattcagaa atatggagga aggtttaaaa gaaaacataa aaatatatag
     2401 aggaggaagg aaactagctg ccctcgattg tctatcttcc attctcacca agtttcaaca
     2461 actttattca gtcattcttc tctggttcat ttcccaacca atcttcccga ataaagccca
     2521 tctgtccttt cggccacatt cctcacctac atccgtactc cttatctccc gttcttatcc
     2581 agtcttttat cgtctctgcg tctcgcacac tgtctcgtcg gtaatgtaaa aaaaaaacaa
     2641 aaaaaacttg aaaacttgcg gaaagatttc ctaataaaag gagacgaatc cggtaggaaa
     2701 tcgacaagtc tctgcttctc gaagactagg tgagttacat ttctttggcc gaattatttt
     2761 gaaatcctaa atttgataat ttcagagatg tctgtaaaac ttgcgtgtta tgtgacggct
     2821 tcggtcactg tcgccactct tatggtatgc ttcatgacca tgtcaaccat ctactcggag
     2881 gttgatggat tcagagagaa gcttgatacc gagatgaatg tgttcagagt gagttaaatt
     2941 atattttgaa ttttaaataa tttttaattt tctagcaatc taccaatgga ttgtggaagg
     3001 acatagttgt catcggaaga tctagcaagc gtgtccgttg tcaatatgaa gagaccaacg
     3061 ctaccccaac tccacatgct gatggatccc catctgctcc accaggtcaa ccaccagcag
     3121 ttccaccagt cttcaaccag ccaaagactc caaatggagc caatggaaat ggaccaacct
     3181 gcaactgcaa tgctgataac aagtgcccag ctggaccatc cggaccaaag ggagttccag
     3241 gagttccagg actcgacgga gttccaggac ttgacggtgt tccaggagtt ggagctgatg
     3301 atatcgctcc acaacgcgag tctgtcggat gcttcacttg cccacaagga ccagttggac
     3361 caccaggagc tcttggaaga ccaggaccac gtggacttcc aggaccaaga ggacaaaatg
     3421 gaaacccagg aagagatgga caaccaggac atccaggaga gcaaggatca tccggtcaaa
     3481 tcggaaagat cggagagcca ggaccaccag gagagaaggg acgcgacgcc gagcatccaa
     3541 tcggaagacc aggaccaaag ggaccaagag gagatcaagg accaacagga ccagctggac
     3601 agaacggtct tcacggacca ccaggagagc caggaaccgt tggaccagaa ggaccatctg
     3661 gaaagcaagg acgtcaagga ccagacggaa cccagggaga gactggacca gacggaagac
     3721 caggaaagga tgccgagtac tgccagtgcc cagacaagtc tccaccatca gaggctgtca
     3781 acgccaaccg tggatacaga aatatctaaa ttgttggtgt tttctaataa aatatttgag
     3841 attcagtgag acttttttct tggcggcaca ataaagtttc ctctttttgt tgggtcactc
     3901 aacgaacaca atacaaatat ttgagcgctc gcatttttct caacctttcg ggtttaaagc
     3961 acttcaaaat ggaattgaag agagattgtg aaagtacatc agacagaatc acattttgat
     4021 agcattcact tcactcagat gcaaactttt cagtgactat aaaaagtcat aaaataataa
     4081 cattaaaaaa gcaaataaat tagcgagaga attttttgac aaaaagaaag aagagtgata
     4141 gagaagaagg gaatgcttga aaggattttg catttatctt aatcaccgtc ttcgagaaga
     4201 cgaaccatgg cgccagtttc agaatccaag tttctaatat agttcatcac aatgttgacg
     4261 ttggtccaac ctccggcttg cataatctct gggatggaaa ctccagcgcg agccatgtca
     4321 cgggcagctc cgacacgggc ggaatgtcca gaccaagcca agtaacgttg tccggaatca
     4381 tccttggctc cgtagatgag acggtgggta gcttcgaaga ttcctgaaaa tttattttca
     4441 gcttattctt agctaatatg ttgtttaaac ttaccttcaa gagctcgagt cgagagttgt
     4501 gatgttgcac tcggggcagc aactccgttc ttgcgaacac gacagaacag gtagttgttt
     4561 ggatcatcgg cgactccgga aaccgaaatc cagcgctcaa cgagcttggt aactccaagg
     4621 gagagcgctt tttcgactcc ggcagtcgag accagcgttt ttgtacgtcc gatgtggatg
     4681 agcattcttc ctccgtctgt gcgactaata tccttgacgc gaatgcgggc aatttcggcg
     4741 atacggagaa gtgtgttgta agcaattccg aggaaggcga ggtttcggat gtcctggcaa
     4801 cgatcggaat tttccataag agaacggacc tgatcgaagt cggttctgaa aatttcgtca
     4861 agtcttagga ttgaatacat taatatttaa acttaccttt cgaaggcgag ggcttgcttg
     4921 gctctctctc cagcatcaac attttccttg cggatacgtc tcattacgag agatacggca
     4981 ttagaatctg atgggcgtgg aagtccagat cggcgatgga gcatattgag ctgtccgaga
     5041 tgttgttgga tcgtcttcac cgccaatcca cgagcttgga ggtagagcaa gtagtcacga
     5101 acgtcttctg gctcggctgg gaaccacttg cggttgttaa gcttgcacca ggcagcccaa
     5161 gaacggcaca ctgagagaag catcttccag gtgtgctcgg agaaggcttg tctgtcacgg
     5221 aacatatcca tgaggttctt gcgaacttca tcacttgtcg cgtcaactgg aagtgctgga
     5281 agattttgat gaacagtgag aagatttgac atattttcga agttttttag atgcactaga
     5341 acaaagcgtg ttggcttcct ctgagcccgc tttccttata tacccgcatt ctgcagcctt
     5401 acagaatgtt ctagaaggtc ctagatgcat tcgtttgaaa atactcccgg tgggtgcaaa
     5461 gagacgcaga cggaaaatgt atctgggtct ctttattgtg tacactactt ttccatgtac
     5521 cgaatgtgag tcgccctcct tttgcaacaa gcagctcgaa tgttctagaa aaaggtggaa
     5581 aatagtataa ataccgttga aaataaatac cgaacaacat ttgctctaat tgtgaaatta
     5641 gaaatcttca aactataatc atgtcacttt accactattt ccgtccagct caacgttccg
     5701 tttttggtga tcatttttgc tttcgtcgta aatctacaca cgcgtctctt ccgtgcgaga
     5761 gtccaagcca gcagccaaat tcgttgactg agtattcaac gtttatacgt tgtcggcaac
     5821 gagaaatagg aaaatgcatc gggaaatgtt cttttttcga ttttttccaa ggttttgaca
     5881 aattttacca cgaattttgc tatgttttca attaaaaaat atgttattca actgtttcta
     5941 tgaggaaaat aaggctttgc atgtaatttt cttattcagc ataattttta attaatttga
     6001 attttctgtc ctaacgttta ttttgttttc ttggttatga ctgatctgaa attaattttt
     6061 gaattttaag gtaatatgtc aggcggtgcc gcaagtttgt acaaaaaagc aggctccatg
     6121 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     6181 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     6241 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     6301 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     6361 gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     6421 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     6481 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     6541 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     6601 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     6661 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     6721 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     6781 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     6841 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     6901 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     6961 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     7021 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     7081 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     7141 tagacccagc tttcttgtac aaagtgggtc caattactct tcaacatccc tacatgctct
     7201 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     7261 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     7321 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     7381 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     7441 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     7501 gtttatgacc gcaattttta ataacttcgt atagcataca ttatacgaag ttattttcag
     7561 ggagccggat ctgattacgg atccggaggt ggcgggatgg cttcttctct tcgtcagatc
     7621 ctcgactctc agaagatgga gtggcgttct aacgctggag gatctggcgg aggaggcagt
     7681 ggtagtggca gtatgcctaa agatccagcc aaacctccgg ccaaggcaca agttgtggga
     7741 tggccaccgg tgagatcata ccggaagaac gtgatggttt cctgccaaaa atcaagcggt
     7801 ggcccggagg cggcggcgtt cgtgaagggt ggtggcggtt ctggctcagg atctgattat
     7861 aaagacgatg acgataagcg tgactacaag gacgacgacg acaagcgtga ttacaaggat
     7921 gacgatgaca agagactagt tcgacctgca gactggctgt gtataaggga gcctgacatt
     7981 tatattcccc agaacatcag gttaatggcg tttttgatgt cattttcgcg gtggctgaga
     8041 tcagccactt cttccccgat aacggagacc ggcacactgg ccatatcggt ggtcatcatg
     8101 cgccagcttt catccccgat atgcaccacc gggtaaagtt cacgggggac tttatctgac
     8161 agcagacgtg cactggccag ggggatcacc atccgtcgcc cgggcgtgtc aataatatca
     8221 ctctgtacat ccacaaacag acgataacgg ctctctcttt tataggtgta aaccttaaac
     8281 tgcatttcac cagcccctgt tctcgtcggc aaaagagccg ttcatttcaa taaaccgggc
     8341 gacctcagcc atcccttcct gattttccgc tttccagcgt tcggcacgca gacgacgggc
     8401 ttcattctgc atggttgtgc ttaccgaacc ggagatattg acatcatata tgccttgagc
     8461 aactgatagc tgtcgctgtc aactgtcact gtaatacgct gcttcatagc atacctcttt
     8521 ttgacatact tcgggtaatc gataacatgg tcatagctgt ttcctgtgtg aaattgttat
     8581 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     8641 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
     8701 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
     8761 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     8821 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     8881 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     8941 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     9001 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     9061 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     9121 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     9181 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     9241 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     9301 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     9361 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     9421 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     9481 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     9541 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     9601 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     9661 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     9721 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     9781 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     9841 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     9901 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     9961 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
    10021 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
    10081 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
    10141 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
    10201 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
    10261 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
    10321 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
    10381 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
    10441 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
    10501 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
    10561 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
    10621 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
    10681 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
    10741 aaaaataggc gtatcacgag gccctttcgt c
//
