LOCUS       sequence-236083-        5021 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  pNG2 backbone with an optimized chloramphenicol resitance marker for
            Rhodococcus opacus PD630.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5021)
  AUTHORS   DeLorenzo DM, Rottinghaus AG, Henson WR, Moon TS
  TITLE     Molecular Toolkit for Gene Expression Control and Genome 
            Modification in Rhodococcus opacus PD630.
  JOURNAL   ACS Synth Biol. 2018 Feb 16;7(2):727-738. doi: 
            10.1021/acssynbio.7b00416. Epub 2018 Feb 5.
  PUBMED    29366319
REFERENCE   2  (bases 1 to 5021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acssynbio.7b00416"; journalName: "ACS Synth Biol"; date: 
            "2018-02-16- 16"; volume: "7"; issue: "2"; pages: "727-738"
COMMENT     Sequence Label: pDD112
FEATURES             Location/Qualifiers
     source          1..5021
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      143..170
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      1106..1177
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     promoter        join(1470..1475,1476..1492,1493..1498)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     CDS             1668..2201
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLWSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     oriT            2289..2398
                     /note="incP origin of transfer"
ORIGIN
        1 agctccaagt cagatatttc gctgaggggc caccccacaa ctgcacactc ccccgctctc
       61 ccgtcgagcc ctggtggtgg aacaccagcg acagccgagc acccccaacc acctgtacca
      121 accactagtt gattagctaa gcagaaggcc atcctgacgg atggcctttt tgcgtttaat
      181 actgtttaaa ctctagaaat attggatcgt cggcaccgtc acggccgtgg gaggcggcac
      241 gatccgcgac gtgatgatcg gccgcatccc cacggtgctg cgcagtgagc tctacgccat
      301 cccggcgttg atctgtgcgt tcgctgtgcg ggctctaaca cgtcctagta tggtaggatg
      361 agcaacattt cgacgccgag agattcgccg cccgaaatga gcacgatccg catgcttaat
      421 taagaaggag atatacatat ggagaaaaag atcacggggt acaccaccgt ggacatcagc
      481 cagtggcacc gtaaggaaca tttcgaagcg ttccagtccg tcgcgcaatg tacgtacaat
      541 cagaccgtcc aactcgatat tacggcgttt ctgaagaccg tgaagaagaa taagcacaaa
      601 ttttaccccg cctttattca tatcctggcg cggctgatga acgcccatcc agaatttcgc
      661 atggccatga aagatggcga gttggtcatc tgggatagcg tccatccatg ttatacggtg
      721 ttccacgagc agaccgaaac cttttcgagc ttgtggagcg agtatcacga tgatttccgg
      781 cagttcttgc acatttatag ccaagatgtg gcctgctatg gtgagaatct ggcctacttt
      841 ccgaaaggtt tcattgagaa catgtttttt gtgtccgcca atccgtgggt ctcgttcacg
      901 agcttcgacc tcaacgtcgc gaatatggat aacttttttg cgccggtctt taccatgggg
      961 aaatattata cgcagggcga taaggtgctg atgccactcg cgatccaagt gcatcatgcc
     1021 gtctgcgatg gcttccacgt cggtcggatg ttgaacgaac tccaacaata ttgcgatgag
     1081 tggcaaggtg gtgcgtaagc tatgccaaat aaaacgaaag gctcagtcga aagactgggc
     1141 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctga ggactgtctt ctttatcatg
     1201 caactcgtag gacaggtgcc acttcatccg gggtcagcac caccggcaag cgccgcgacg
     1261 gccgaggtct tccgatctcc tgaagccagg gcagatccgt gcacagcacc ttgccgtaga
     1321 agaacagcaa ggccgccaat gcctgacgat gcgtggagac cgaaaccttg cgctcgttcg
     1381 ccagccagga cagaaatgcc tcgacttcgc tgctgcccaa ggttgccggg tgacgcacac
     1441 cgtggaaacg gatgaaggca cgaacccagt tgacataagc ctgttcggtt cgtaaactgt
     1501 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
     1561 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttgta cagtctatgc
     1621 ctcgggcatc caagcagcaa gcgcgttacg ccgtgggtcg atgtttgatg ttatggagca
     1681 gcaacgatgt tacgcagcag ggcagtcgcc ctaaaacaaa gttaggtggc tcaagtatgg
     1741 gcatcattcg cacatgtagg ctcggccctg accaagtcaa atccatgcgg gctgctcttg
     1801 atcttttcgg tcgtgagttc ggagacgtag ccacctactc ccaacatcag ccggactccg
     1861 attacctcgg gaacttgctc cgtagtaaga cattcatcgc gcttgctgcc ttcgaccaag
     1921 aagcggttgt tggtgctctc gcggcttacg ttctgcccaa gtttgagcag ccgcgtagtg
     1981 agatctatat ctatgatctc gcagtctccg gcgagcaccg gaggcagggc attgccaccg
     2041 cgctcatcaa tctcctcaag catgaggcca acgcgcttgg tgcttatgtg atctacgtgc
     2101 aagcagatta cggtgacgat cccgcagtgg ctctctatac aaagttgggc atacgggaag
     2161 aagtgatgca ctttgatatc gacccaagta ccgccaccta acaattcgtt caagccgaga
     2221 tcggcttccc ggccgcggct tggtttcatc agccatccgc ttgccctcat ctgttacgcc
     2281 ggcggtagcc ggccagcctc gcagagcagg attcccgttg agcaccgcca ggtgcgaata
     2341 agggacagtg aagaaggaac acccgctcgc gggtgggcct acttcaccta tcctgcccgg
     2401 ctgacgccgt tggatacacc aaggaaagtc tacacgaacc ctttggcaaa atcctgtata
     2461 tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata atgaccccga agcagggtta
     2521 tgcagcggaa aagcgctgct tccctgctgt tttgtgggcc gcaaagcgct ccggcttaac
     2581 tcaggcgtta gtcccatcac ccgctttatg cgcgcagcca cttatttcag cgctccgccc
     2641 tgttccaaag gctttctagc cttttgggcc tccagcgctt gtgcagcaag cgccaccagc
     2701 tatccttctt gcagcgctgc gccgctttcg tggtccagcg gtttttcttg ggctgcgccc
     2761 ttcttcaagg ccgggcgctc ccttttggcc ttcgggtcta actctgcggt caagcggacc
     2821 cgcattctgc gggccgctta ccttggccgt tagacatcat ggtaaatctg cgcagacagc
     2881 cctgtgcagc tgaaacgcgg ttacgtatag cttgccatat gtctagccat acgtaaccgc
     2941 aggtaaaagg catatttttc gcgtgtcatg gctagtaaat aacaccggtg tcatttagag
     3001 tcagggaaag acaatgaaaa acgaagaaag ccaccgggcg gcaacccgat gactttcgct
     3061 tatcacccag cacacacctg ggagaaatca cggtcatgag tttacagact catgcgcaga
     3121 atgcgcacac taaaacacct acccgcgtcg agcgcgaccg tggtggactg gacaacaccc
     3181 cagcatctgc cagtgaccgc gaccttttac gcgatcatct aggccgcgat gtactccacg
     3241 gttcagtcac acgagacttt aaaaaggcct atcgacgcaa cgctgacggc acgaactcgc
     3301 cgcgtatgta tcgcttcgag actgatgctt taggacggtg cgagtacgcc atgctcacca
     3361 ccaagcagta cgccgccgtc ctggtcgtag acgttgacca agtaggtacc gcaggcggtg
     3421 accccgcaga cttaaacccg tacgtccgcg acgtggtgcg ctcactgatt actcatagcg
     3481 tcgggccagc ctgggtgggt attaacccaa ctaacggcaa agcccagttc atatggctta
     3541 ttgaccctgt ctacgctgac cgtaacggta aatctgcgca gatgaagctt cttgcagcaa
     3601 ccacgcgtgt gctgggtgag cttttagacc atgacccgca cttttcccac cgctttagcc
     3661 gcaacccgtt ctacacaggc aaagccccta ccgcttatcg ttggtatagg cagcacaacc
     3721 gggtgatgcg ccttggagac ttgataaagc aggtaaggga tatggcagga cacgaccagt
     3781 tcaaccccac cccacgccag caattcagct ctggccgcga acttatcaac gcggtcaaga
     3841 cccgccgtga agaagcccaa gcattcaaag cactcgccca ggacgtagac gcggaaatcg
     3901 ccggtggtct cgaccagtat gacccggaac ttatcgacgg tgtgcgtgtg ctctggattg
     3961 tccaaggaac cgcagcacgc gacgaaacag cctttagaca tgcgcttaag actggccacc
     4021 gcttgcgcca gcaaggccaa cgcctgacag acgcagcaat catcgacgcc tatgagcacg
     4081 cctacaacgt cgcacacacc cacggcggtg caggccgcga caacgagatg ccacccatgc
     4141 gcgaccgcca aaccatggca aggcgcgtgc gcgggtatgt cgcccaatcc aagagcgaga
     4201 cctacagcgg ctctaacgca ccaggtaaag ccaccagcag cgagcggaaa gccttggcca
     4261 cgatgggacg cagaggcgga caaaaagccg cacaacgctg gaaaacagac cccgagggca
     4321 aatatgcgca agcacaaagg tcgaagcttg aaaagacgca ccgtaagaaa aaggctcaag
     4381 gacgatctac gaagtcccgt attagccaaa tggtgaacga tcagtatttc cagacaggga
     4441 cagttcccac gtgggctgaa ataggggcag aggtaggagt ctctcgcgcc acggttgcta
     4501 ggcatgtcgc ggagctaaag aagagcggtg actatccgga cgtttaaggg gtctcatacc
     4561 gtaagcaata tacggttccc ctgccgttag gcagttagat aaaacctcac ttgaagaaaa
     4621 ccttgagggg cagggcagct tatatgcttc aaagcatgac ttcctctgtt ctcctagacc
     4681 tcgcaaccct ccgccataac ctcaccgaat tgcttaagtt tcaccccttt gacggcttaa
     4741 ccgccgcata ggaaatgccc tccggactaa ttgtctggag ggcatttttg ctacctgctg
     4801 tgcactttgt acataaggca acaagatttt ggcaaaaggt ggaagggcga attgctgcgc
     4861 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccat tcgccattca
     4921 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     4981 cgaaaggggg atgtgctgca aggcgattaa gttgggtaac g
//
