LOCUS       sequence-236155-        2178 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly AB part for pLux76B (Adam Meyer's 
            improved pLux promoter from https://doi.org/10.1101/285866, selected
            for dynamic range and less cross-sensitivity)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2178)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: P59m
FEATURES             Location/Qualifiers
     source          1..2178
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..32
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(36..79)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    82..103
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    203..223
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(229..246)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(400..988)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(480..499)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1159..1950,1951..2019))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1782..1801
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2020..2124)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attaacctat aaaaataggc gtatcacgag gcagaatttc agataaaaaa aatccttagc
       61 tttcgctaag gatgatttct ggaattcgcg gccgcttcta gagactagtg ggtctcagga
      121 gaacgatcgt tggctgacct gtaggatctt acaagtttac gcaagaaaat ggtttgttac
      181 tttcgaataa atactagaga cctactagta gcggccgctg caggcttcct cgctcactga
      241 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat
      301 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      361 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccacaggc tccgcccccc
      421 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      481 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      541 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
      601 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
      661 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
      721 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
      781 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
      841 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
      901 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
      961 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga
     1021 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat
     1081 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga
     1141 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg
     1201 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga
     1261 gggcttacca tctggcccca gtgctgcaat gataccgcgc gacccacgct caccggctcc
     1321 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac
     1381 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc
     1441 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc
     1501 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc
     1561 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt
     1621 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc
     1681 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg
     1741 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag
     1801 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat
     1861 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc
     1921 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa
     1981 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta
     2041 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa
     2101 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga
     2161 aaccattatt atcatgac
//
