LOCUS       sequence-236176-        2192 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly BC part for BCD13 (Medium expression 
            bi-cistronic RBS, engineered for downstream context-independence; 
            see https://doi.org/10.1038/nmeth.2404)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2192)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: U3m
FEATURES             Location/Qualifiers
     source          1..2192
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     29..47
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(51..94)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    97..118
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    232..252
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(258..275)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(429..1017)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(509..528)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1188..1979,1980..2048))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1811..1830
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2049..2153)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cattattatc atgacattaa cctataaaaa taggcgtatc acgaggcaga atttcagata
       61 aaaaaaatcc ttagctttcg ctaaggatga tttctggaat tcgcggccgc ttctagagac
      121 tagtgggtct catactgggc ccaagttcac ttaaaaagga gatcaacaat gaaagcaatt
      181 ttcgtactga aacatcttaa tcatgcaatg gaggctttct aatgagagac ctactagtag
      241 cggccgctgc aggcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
      301 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
      361 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      421 ctggcgtttt tccacaggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      481 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
      541 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
      601 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
      661 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
      721 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
      781 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
      841 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
      901 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
      961 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1021 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1081 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1141 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1201 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1261 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1321 ataccgcgcg acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1381 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1441 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1501 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     1561 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     1621 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     1681 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     1741 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     1801 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     1861 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     1921 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     1981 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2041 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2101 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2161 ccccgaaaag tgccacctga cgtctaagaa ac
//
