LOCUS       sequence-236225-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression plasmid for PURE system components.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Shimizu Y, Inoue A, Tomari Y, Suzuki T, Yokogawa T, Nishikawa K, 
            Ueda T
  TITLE     Cell-free translation reconstituted with purified components.
  JOURNAL   Nat Biotechnol. 2001 Aug;19(8):751-5. doi: 10.1038/90802.
  PUBMED    11479568
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Biotechnol"; date: "2001-08"; volume: "19"; issue: "8"; pages: 
            "751-5"
COMMENT     Sequence Label: pET21a-LysRS-His
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     44..62
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(115..132)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tmncaaaacc cctcagaccc gtttagaggc cccaaggggt tatgctagtt attgctcagc
       61 ggtggcagca gccaactcag cttcctttcg ggctttgtta gcagccggat ctcagtggtg
      121 gtggtggtgg tgctcgagtt tctgtgggcg catcgccggg aagagaataa cgtcgcgaat
      181 agtatggctg ttagtaaaca gcataatcat tcggtcgata ccaataccca gaccagcggt
      241 tggcggcaga ccatattcca gcgcagtcac gtaatcttcg tcatagaaca tggcttcgtc
      301 gtcacctgca gctttagcat taacctgttc ctggaaacgt tcagcctgat cttctgcgtc
      361 gtttaattcg ctaaaaccat taccgatttc acgaccaccg atgaagaatt caaaacggtc
      421 ggtgatttcc gggttaacat cattacggcg tgccagcggg gacacttctg ccggatattc
      481 cgtaataaag gttggctgaa tcagatgtgc ttctgccact tcatcaaaga tctctgtgac
      541 aatacgtccc aacccccagc ttttctctac cgtaataccg atagattcag ctaatgcttt
      601 agcagcatca aaattatcca ggtcggccat atcggtttct ggacgatatt ttttgattgc
      661 ttcgcgcatg gtgagttttt caaacggttt gccgaaatca aacacatgct cgccataagt
      721 gactttagtg gtacccagaa cctcttgtgc cagggtgcgg aacagtgact ctgtcagttc
      781 aatcaaatcg tggtaatccg catacgccat gtagagttcc atcattgtga actcaggatt
      841 atggcgaaca gaaatacctt cattacggaa gttacggttg atttcgaata cccgttcaaa
      901 accgcctaca accagacgtt tcagatacag ctccggcgcg atacgcagat acatatctaa
      961 atccagagca ttatgatggg taataaacgg gcgagcagat gccccacctg gaattacctg
     1021 catcatcggg gtttctactt ccataaagcc gcgcgcgacc atgaattgac ggatagcggc
     1081 cagaattttt gaacggacaa caaacgtttg acgggattat cgttagcgat gaggtccaga
     1141 taacgttgac gataacggac ttcctgatcc tgcaaana
//
