LOCUS       sequence-236358-       11810 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express 3xNLS-NGN2 TALE-GCN4 engineered to bind a site in the human 
            NGN2 ene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11810)
  AUTHORS   Zhao C, Zhang Y, Zhao Y, Ying Y, Ai R, Zhang J, Wang Y
  TITLE     Multiple Chemical Inducible Tal Effectors for Genome Editing and 
            Transcription Activation.
  JOURNAL   ACS Chem Biol. 2018 Mar 16;13(3):609-617. doi: 
            10.1021/acschembio.7b00606. Epub 2018 Jan 8.
  PUBMED    29308880
REFERENCE   2  (bases 1 to 11810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acschembio.7b00606"; journalName: "ACS Chem Biol"; date: 
            "2018-03-16- 16"; volume: "13"; issue: "3"; pages: "609-617"
COMMENT     Sequence Label: EF1a-3NLS-NGN2TALE-GCN4
FEATURES             Location/Qualifiers
     source          1..11810
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        130..341
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      1205..1214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1226..1246
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1250..1270
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1274..1294
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4001..4057
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4073..4129
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4145..4201
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4217..4273
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4289..4345
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4361..4417
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4433..4489
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4505..4561
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4577..4633
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4649..4705
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     primer_bind     complement(4929..4949)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4934..5263
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5114..5249
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5273..5647
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     misc_feature    5733..6321
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5786..5806)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6204..6215)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6408..6641
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6713..6834
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6853..6988
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7008..7027)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7009..7027)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7037..7054)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7037..7053)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7046..7068)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7195..7650
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7282..7301)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7492..7513
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7676..7780
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7781..7849,7850..8641)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7999..8018)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8812..9400
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9301..9320
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9554..9571
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9688..9709
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9724..9729,9730..9747,9748..9754)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9762..9778
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9767..9789
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9786..9802
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9786..9802
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9821..9841
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9823..9841
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        9869..10095
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             10096..10276
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    10323..10448
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10941..11174
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             11359..11403
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    11670..11787
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 atagcccggg ctcgaccata tgataagctt tgcaaagatg gataaagttt taaacagaga
       61 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg
      121 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
      181 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
      241 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
      301 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt
      361 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct
      421 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga
      481 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct
      541 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt
      601 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc
      661 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg
      721 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac
      781 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc
      841 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag
      901 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct
      961 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg
     1021 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga
     1081 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc
     1141 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctcctttc
     1201 tagagccacc atgggtggcg cgcctccaaa aaagaagaga aaggtagatc caaaaaagaa
     1261 gagaaaggta gatccaaaaa agaagagaaa ggtaggatta attaatggaa gtggaagtgg
     1321 aagtggaggt accccggatg ttccagatta cgcttcgcgg acccggctcc cttccccacc
     1381 cgcacccagc ccagcgtttt cggccgactc gttctcagac ctgcttaggc agttcgaccc
     1441 ctcactgttt aacacatcgt tgttcgactc ccttcctccg tttggggcgc accatacgga
     1501 ggcggccacc ggggagtggg atgaggtgca gtcgggattg agagctgcgg atgcaccacc
     1561 cccaaccatg cgggtggccg tcaccgctgc ccgaccgccg agggcgaagc ccgcaccaag
     1621 gcggagggca gcgcaaccgt ccgacgcaag ccccgcagcg caagtagatt tgagaacttt
     1681 gggatattca cagcagcagc aggaaaagat caagcccaaa gtgaggtcga cagtcgcgca
     1741 gcatcacgaa gcgctggtgg gtcatgggtt tacacatgcc cacatcgtag ccttgtcgca
     1801 gcaccctgca gcccttggca cggtcgccgt caagtaccag gacatgattg cggcgttgcc
     1861 ggaagccaca catgaggcga tcgtcggtgt ggggaaacag tggagcggag cccgagcgct
     1921 tgaggccctg ttgacggtcg cgggagagct gagagggcct ccccttcagc tggacacggg
     1981 ccagttgctg aagatcgcga agcggggagg agtcacggcg gtcgaggcgg tgcacgcgtg
     2041 gcgcaatgcg ctcacgggag cacccctcaa cctgacccca gagcaggtcg tggcaattgc
     2101 gagccataac gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     2161 ccaagcgcac ggacttacgc cagagcaggt cgtggcaatt gcgagcaaca tcgggggaaa
     2221 gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc acggactaac
     2281 cccagagcag gtcgtggcaa ttgcgagcaa catcggggga aagcaggcac tcgaaaccgt
     2341 ccagaggttg ctgcctgtgc tgtgccaagc gcacgggttg accccagagc aggtcgtggc
     2401 aattgcgagc aacggagggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     2461 gctgtgccaa gcgcacggct taaccccaga gcaggtcgtg gcaattgcga gccataacgg
     2521 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     2581 cctgacccca gagcaggtcg tggcaattgc gagcaacatc gggggaaagc aggcactcga
     2641 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggactgacac cagagcaggt
     2701 cgtggcaatt gcgagcaacg gagggggaaa gcaggcactc gaaaccgtcc agaggttgct
     2761 gcctgtgctg tgccaagcgc acggactcac tccagagcag gtcgtggcaa ttgcgagcca
     2821 taacggggga aagcaggcac tcgaaaccgt ccagaggttg ctgcctgtgc tgtgccaagc
     2881 gcacggcctc accacagagc aggtcgtggc aattgcgagc aacatcgggg gaaagcaggc
     2941 actcgaaacc gtccagaggt tgctgcctgt gctgtgccaa gcgcacggat taaccccaga
     3001 gcaggtcgtg gcaattgcga gcaacggagg gggaaagcag gcactcgaaa ccgtccagag
     3061 gttgctgcct gtgctgtgcc aagcgcacgg acttacccca gagcaggtcg tggcaattgc
     3121 gagcaacatc gggggaaagc aggcactcga aaccgtccag aggttgctgc ctgtgctgtg
     3181 ccaagcgcac gggctcaccc cagagcaggt cgtggcaatt gcgagcaaca tcgggggaaa
     3241 gcaggcactc gaaaccgtcc agaggttgct gcctgtgctg tgccaagcgc acggtcttac
     3301 cccagagcag gtcgtggcaa ttgcgagcaa cggaggggga aagcaggcac tcgaaaccgt
     3361 ccagaggttg ctgcctgtgc tgtgccaagc gcacggcctc accccagagc aggtcgtggc
     3421 aattgcgagc aacatcgggg gaaagcaggc actcgaaacc gtccagaggt tgctgcctgt
     3481 gctgtgccaa gcgcatgggc tcaccccaga gcaggtcgtg gcaattgcga gccatgacgg
     3541 gggaaagcag gcactcgaaa ccgtccagag gttgctgcct gtgctgtgcc aagcgcacgg
     3601 tctaacccca gagcaggtcg tggcaattgc gagccataac gggggaaagc aggcactcga
     3661 aaccgtccag aggttgctgc ctgtgctgtg ccaagcgcac ggactcacgc ctgagcaggt
     3721 agtggctatt gcatccaata acgggggcag acccgcactg gagtcaatcg tggcccagct
     3781 ttcgaggccg gaccccgcgc tggccgcact cactaatgat catcttgtag cgctggcctg
     3841 cctcggcgga cgacccgcct tggatgcggt gaagaagggg ctcccgcacg cgcctgcatt
     3901 gattaagcgg accaacagaa ggattcccga gaggacatca catcgagtgg cagctagcgg
     3961 cggatccgcc atcatacacg cggccgcaac aagcggtccg gaagaacttt tgagcaagaa
     4021 ttatcatctt gagaacgaag tggctcgtct taagaaaggt tctggcagtg gagaagaact
     4081 gctttcaaag aattaccacc tggaaaatga ggtagctaga ctgaaaaagg ggagcggaag
     4141 tggggaggag ttgctgagca aaaattatca tttggagaac gaagtagcac gactaaagaa
     4201 agggtccgga tcgggtgagg agttactctc gaaaaattat catctcgaaa acgaagtggc
     4261 tcggctaaaa aagggcagtg gttctggaga agagctatta tctaaaaact accacctcga
     4321 aaatgaggtg gcacgcttaa aaaagggaag tggcagtggt gaagagctac tatccaagaa
     4381 ttatcatctt gagaacgagg tagcgcgttt gaagaagggt tccggctcag gagaggaact
     4441 gctctcgaag aactatcatc ttgaaaatga ggtcgctcga ttaaaaaagg gatcgggcag
     4501 tggtgaggaa ctactttcaa agaattacca cctcgaaaac gaagtagctc gattaaagaa
     4561 aggttcaggg tcgggtgaag aattactgag taaaaattat catctggaaa atgaggtagc
     4621 gagactaaaa aaggggagtg gttctggcga ggaattgcta tcgaaaaatt atcatcttga
     4681 gaacgaagtt gctaggctca aaaagggctc aggctcaggc tgagtttaaa catcgcctgc
     4741 aggggaagtg gaagtgatat cggaagtgga agcactagtg gaagtttcga agtcgacttg
     4801 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4861 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatgtc
     4921 gaccctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc cagcaggcag
     4981 aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt ccccaggctc
     5041 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca tagtcccgcc
     5101 cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg
     5161 ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca
     5221 gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta ccatggccaa
     5281 gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg
     5341 gaccgaccgg ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg
     5401 ggacgacgtg accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct
     5461 ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc
     5521 cacgaacttc cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg
     5581 gcgggagttc gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca
     5641 ggactgaccg atcgataaaa taaaagattt tatttagtct ccagaaaaag gggggaatga
     5701 aagaccccac ctgtaggttt ggcaagctcg acaatcaacc tctggattac aaaatttgtg
     5761 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     5821 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     5881 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     5941 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6001 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6061 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6121 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6181 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6241 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6301 ccctttgggc cgcctccccg cctggaattc gagctcagta cctttaagac caatgactta
     6361 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     6421 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     6481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     6541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     6601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt catgtcatct
     6661 tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga ggaacttgtt
     6721 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     6781 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     6841 ctggctctag ctatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     6901 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     6961 aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt
     7021 cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     7081 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     7141 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc
     7201 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     7261 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     7321 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     7381 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     7441 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     7501 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     7561 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     7621 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
     7681 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7741 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7801 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     7861 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     7921 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     7981 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8041 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8101 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8161 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8221 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8281 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8341 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8401 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8461 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8521 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8581 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8641 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8701 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8761 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     8821 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     8881 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     8941 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9001 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9061 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     9121 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccaa
     9181 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     9241 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     9301 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     9361 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     9421 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     9481 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     9541 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     9601 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     9661 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     9721 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     9781 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     9841 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     9901 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc
     9961 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
    10021 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
    10081 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
    10141 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
    10201 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
    10261 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
    10321 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
    10381 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
    10441 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
    10501 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
    10561 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
    10621 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
    10681 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
    10741 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
    10801 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
    10861 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
    10921 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
    10981 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
    11041 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
    11101 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
    11161 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
    11221 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
    11281 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
    11341 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
    11401 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
    11461 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    11521 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    11581 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    11641 gtgaacggat ctcgacggta tcggttaact tttaaaagaa aaggggggat tggggggtac
    11701 agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa
    11761 aaacaaatta caaaaattca aaattttatc gatcacgaga ctagcctcga
//
