LOCUS       sequence-23672-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Kapust RB, Toezser J, Copeland TD, Waugh DS
  TITLE     The P1' specificity of tobacco etch virus protease.
  JOURNAL   Biochem Biophys Res Commun. 2002 Jun 28. 294(5):949-55.
  PUBMED    12074568
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochem 
            Biophys Res Commun. 2002 Jun 28. 294(5):949-55."
COMMENT     Sequence Label: pKM631
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             26..46
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             788..805
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    complement(811..835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(847..864)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(847..863)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(856..878)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 tgacagnttg tcaaaagcag gctcggagaa cctgtacttc cagggtagcg agcaacaggt
       61 tcaggaactt gagaaaaagt ggtacgcact gcaggtagag cccggaaaag agaacgaagc
      121 taaggaaaac ttactgaaag ttctggaact ggaaggttta aaggacctcg ttgacgaggt
      181 aatagttccc gcagaggaaa aggtagtcat aagggctcag ggcaaggaaa agtacaggct
      241 ctctctcaag ggaaacgcga gggacatatc cgttttaggg aagaaaggtg ttaccacatt
      301 caggatagag aacggagaag ttaaggtcgt tgagagtgtt gaaggagaca cttgcgtaaa
      361 cgcccctccc atatcaaaac ccggacaaaa gatcacgtgt aaggaaaata aaacggaagc
      421 aaagatagtt ctggataaca aaatatttcc cggatacata ctcataaagg ctcacatgaa
      481 cgacaagctc ctcatggcta ttgagaaaac tccccacgtc ttcagacctg taatggtggg
      541 cggaaagccc gttcctctga aagaggaaga ggttcaaaac attctcaatc aaataaagag
      601 gggtgttaaa ccttccaagg ttgagtttga aaaaggagat caggtaaggg ttatagaagg
      661 accgtttatg aactttacgg gaactgttga ggaagttcat cccgagaaga ggaaattaac
      721 tgttatgata agtattttcg gaaggatgac tccggtagaa ctggactttg accaagtgga
      781 aaagattcat caccaccatc atcactaata acccagcttt cttgtacaaa gtggtgatag
      841 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa anccctggcg tttcccaact
      901 taatcgcctt gcagcacatc ccccctttcg ccagcttgcg taatagcgaa naggcccgca
      961 ccgatc
//
