LOCUS       sequence-236888-        3606 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  MoClo golden gate assembly CD part for Bxb1 integrase. See DOI: 
            10.1007/s00438-006-0129-5. Please see Supplemental Documents for 
            annotated Genbank file..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3606)
  TITLE     CIDAR MoClo Extension
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: C31m
FEATURES             Location/Qualifiers
     source          1..3606
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     28..46
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(50..93)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    96..117
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     CDS             130..1635
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MVRALVVIRLSRVTDATTSPERQLESCQQLCAQRGWDVVGVAEDL
                     DVSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKL
                     VVSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWG
                     YLPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQ
                     GREPQGREWSATALKRSMISGAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRA
                     ELVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGT
                     VAMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLIGSPAYRA
                     GSPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRS
                     MNVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     primer_bind     complement(1671..1688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1842..2430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1922..1941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2601..3392,3393..3461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3224..3243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3462..3566)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 attattatca tgacattaac ctataaaaat aggcgtatca cgaggcagaa tttcagataa
       61 aaaaaatcct tagctttcgc taaggatgat ttctggaatt cgcggccgct tctagagact
      121 aggtctcaaa tggtgagagc cctggtagtc atccgcctgt cccgcgtcac cgatgctacg
      181 acttcaccgg agcgtcagct ggagtcttgc cagcagctct gcgcccagcg cggctgggac
      241 gtcgtcgggg tagcggagga tctggacgtc tccggggcgg tcgatccgtt cgaccggaag
      301 cgcagaccga acctggcccg gtggctagcg ttcgaggagc aaccgtttga cgtgatcgtg
      361 gcgtaccggg tagatcggtt gacccgatcg atccggcatc ttcagcagct ggtccactgg
      421 gccgaggacc acaagaagct ggtcgtctcc gcgaccgaag cgcacttcga tacgacgacg
      481 ccgtttgcgg cggtcgtcat cgcgcttatg ggaacggtgg cgcagatgga attagaagcg
      541 atcaaagagc ggaaccgttc ggctgcgcat ttcaatatcc gcgccgggaa ataccgagga
      601 tccctgccgc cgtggggata cctgcctacg cgcgtggacg gggagtggcg gctggtgccg
      661 gaccctgtgc agcgagagcg catcctcgag gtgtatcacc gcgtcgtcga caaccacgag
      721 ccgctgcatc tggtggccca cgacctgaac cggcgtggtg tcctgtcgcc gaaggactac
      781 ttcgcgcagc tgcaaggccg cgagccgcag ggccgggagt ggtcggctac cgcgctgaag
      841 cgatcgatga tctccggggc gatgctcggg tacgcgactc tgaacggtaa gaccgtccga
      901 gacgacgacg gagccccgct ggtgcgggct gagccgatcc tgacccgtga gcagctggag
      961 gcgctgcgcg ccgagctcgt caagacctcc cgggcgaagc ccgcggtgtc taccccgtcg
     1021 ctgctgctgc gggtgttgtt ctgcgcggtg tgcggggagc ccgcgtacaa gttcgccggg
     1081 ggaggacgta agcacccgcg ctaccgctgc cgctcgatgg ggttcccgaa gcactgcggg
     1141 aacggcacgg tggcgatggc cgagtgggac gcgttctgcg aggagcaggt actggatctg
     1201 ctcggggacg cggagcgtct ggagaaagtc tgggtagcgg gctcggactc cgcggtcgaa
     1261 ctcgcggagg tgaacgcgga gctggtggac ctgacgtcgc tgatcggctc cccggcctac
     1321 cgggcgggct ctccgcagcg agaagcactg gatgcccgta ttgcggcgct ggccgcgcgg
     1381 caagaggagc tggagggcct ggaggctcgc ccgtctggct gggagtggcg cgaaaccggg
     1441 cagcggttcg gggactggtg gcgggagcag gacaccgcgg caaagaacac ctggcttcgg
     1501 tcgatgaacg ttcggctgac gttcgacgtc cgcggcgggc tgactcgcac gatcgacttc
     1561 ggggatcttc aggagtacga gcagcatctc aggctcggca gcgtggtcga acggctacac
     1621 accgggatgt cgtaaaggtt gagaccctag tagcggccgc tgcaggcttc ctcgctcact
     1681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccacag gctccgcccc
     1861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     1921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     1981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2701 gagggcttac catctggccc cagtgctgca atgataccgc gcgacccacg ctcaccggct
     2761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     2881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     2941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     3601 gaaacc
//
