LOCUS       sequence-23689-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ20 (miR-1003 minigene)
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             413..454
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             464..481
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(504..521)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    523..657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(547..566)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     601..620
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tctctantta agccgatagc gtgcggaaat cgaaaagacc gagcgtgaac gactgcaaca
       61 gcaggaacgc gaggatgaga tgcgcgaact ggccctcaag ctgcgcagaa ggtgggtatc
      121 tggatgtggt tggctctggc ggtcctctca catttacata ttcacaggcg ccgtgagctg
      181 cgtcacaaat atggaacgcc ctcgagtgga aagctctcgg acagcgatgc cgaatctgtg
      241 gcatcggaaa gccagctggc aaagtcgtcg acgcgtcgca gtcgcagcag atcggagagt
      301 cgtaggcggt cgccaccgcc ggaaacacag cacagcagac ggagcagcag ccgaacggaa
      361 aggaggagac gcacaaggac ggcggccgct cgagtctaga gggcccttcg aaggtaagcc
      421 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
      481 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aagatccaga catgataaga
      541 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
      601 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttcta
      661 gagtcggtgg gcctcggggg cgggtgcggg gtcggcgggg ccgccccggg tggcttcggt
      721 cggagccatg gggtcgtgcg ctcctttcgg tcgggcgctg cgggtcgtgg ggcgggcgtc
      781 aggcaccggg cttgcgggtc atgcaccagg tgcgcggtcc ttcgggcacc tcgacgtcgg
      841 cggtgacgtg ancgagcntc taaggagtgg gccgantcag aggg
//
