LOCUS       sequence-23693-e         911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Ruby JG, Jan CH, Bartel DP
  TITLE     Intronic microRNA precursors that bypass Drosha processing.
  JOURNAL   Nature. 2007 Jul 5. 448(7149):83-6.
  PUBMED    17589500
REFERENCE   2  (bases 1 to 911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2007 Jul 5. 448(7149):83-6."
COMMENT     Sequence Label: CJ26 (miR-1003 3'ssAG->GA)
FEATURES             Location/Qualifiers
     source          1..911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             410..451
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             461..478
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(501..518)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    520..654
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(544..563)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     598..617
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ctctgttaag ccgatagcgt gcggaaatcg aaaagaccga gcgtgaacga ctgcaacagc
       61 aggaacgcga ggatgagatg cgcgaactgg ccctcaagct gcgcagaagg tgggtatctg
      121 gatgtggttg gctctggcgg tcctctcaca tttacatatt cacgacgccg tgagctgcgt
      181 cacaaatatg gaacgccctc gagtggaaag ctctcggaca gcgatgccga atctgtggca
      241 tcggaaagcc agctggcaaa gtcgtcgacg cgtcgcagtc gcagcagatc ggagagtcgt
      301 aggcggtcgc caccgccgga aacacagcac agcagacgga gcagcagccg aacggaaagg
      361 aggagacgca caaggacggc ggccgctcga gtctagaggg cccttcgaag gtaagcctat
      421 ccctaaccct ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg
      481 agtttaaacc cgctgatcag cctcgactgt gccttctaag atccagacat gataagatac
      541 attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa
      601 atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca agttctagag
      661 tcggtgggcc tcgggggcgg gtgcggggtc ggcggggccg ccccgggtgg cttcggtcgg
      721 agccatgggg tcgtgcgctc ctttcggtcg ggcgctgcgg gtcgtggggc gggcgtcagg
      781 caccgggctt gcgggtcatg caccaggtgc gcggtccttc gggcacctcg acgtcggcgg
      841 tgacggtgaa nccgaccgtc taaanggagt gcggccgagt tcagagcgga ccgnggtcgc
      901 gctcatcgga g
//
